Iocasia fonsfrigidae NS-1 gen. nov., sp. nov., a Novel Deep-Sea Bacterium Possessing Diverse Carbohydrate Metabolic Pathways

Resolving metabolisms of deep-sea microorganisms is crucial for understanding ocean energy cycling. Here, a strictly anaerobic, Gram-negative strain NS-1 was isolated from the deep-sea cold seep in the South China Sea. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain NS-1...

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Autores principales:	Jing Zhang, Yuechao Zhang, Rui Liu, Ruining Cai, Fanghua Liu, Chaomin Sun
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	fermentation hydrogen carbohydrates cold seep microcompartment Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/97b993e21ab94bbc907c043ace0cd924
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spelling	oai:doaj.org-article:97b993e21ab94bbc907c043ace0cd9242021-11-30T18:30:14ZIocasia fonsfrigidae NS-1 gen. nov., sp. nov., a Novel Deep-Sea Bacterium Possessing Diverse Carbohydrate Metabolic Pathways1664-302X10.3389/fmicb.2021.725159https://doaj.org/article/97b993e21ab94bbc907c043ace0cd9242021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.725159/fullhttps://doaj.org/toc/1664-302XResolving metabolisms of deep-sea microorganisms is crucial for understanding ocean energy cycling. Here, a strictly anaerobic, Gram-negative strain NS-1 was isolated from the deep-sea cold seep in the South China Sea. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain NS-1 was most closely related to the type strain Halocella cellulosilytica DSM 7362T (with 92.52% similarity). A combination of phylogenetic, genomic, and physiological traits with strain NS-1, was proposed to be representative of a novel genus in the family Halanaerobiaceae, for which Iocasia fonsfrigidae NS-1 was named. It is noteworthy that I. fonsfrigidae NS-1 could metabolize multiple carbohydrates including xylan, alginate, starch, and lignin, and thereby produce diverse fermentation products such as hydrogen, lactate, butyrate, and ethanol. The expressions of the key genes responsible for carbohydrate degradation as well as the production of the above small molecular substrates when strain NS-1 cultured under different conditions, were further analyzed by transcriptomic methods. We thus predicted that part of the ecological role of Iocasia sp. is likely in the fermentation of products from the degradation of diverse carbohydrates to produce hydrogen as well as other small molecules, which are in turn utilized by other members of cold seep microbes.Jing ZhangJing ZhangJing ZhangJing ZhangJing ZhangYuechao ZhangRui LiuRui LiuRui LiuRuining CaiRuining CaiRuining CaiRuining CaiFanghua LiuChaomin SunChaomin SunChaomin SunFrontiers Media S.A.articlefermentationhydrogencarbohydratescold seepmicrocompartmentMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	fermentation hydrogen carbohydrates cold seep microcompartment Microbiology QR1-502
spellingShingle	fermentation hydrogen carbohydrates cold seep microcompartment Microbiology QR1-502 Jing Zhang Jing Zhang Jing Zhang Jing Zhang Jing Zhang Yuechao Zhang Rui Liu Rui Liu Rui Liu Ruining Cai Ruining Cai Ruining Cai Ruining Cai Fanghua Liu Chaomin Sun Chaomin Sun Chaomin Sun Iocasia fonsfrigidae NS-1 gen. nov., sp. nov., a Novel Deep-Sea Bacterium Possessing Diverse Carbohydrate Metabolic Pathways
description	Resolving metabolisms of deep-sea microorganisms is crucial for understanding ocean energy cycling. Here, a strictly anaerobic, Gram-negative strain NS-1 was isolated from the deep-sea cold seep in the South China Sea. Phylogenetic analysis based on 16S rRNA gene sequence indicated that strain NS-1 was most closely related to the type strain Halocella cellulosilytica DSM 7362T (with 92.52% similarity). A combination of phylogenetic, genomic, and physiological traits with strain NS-1, was proposed to be representative of a novel genus in the family Halanaerobiaceae, for which Iocasia fonsfrigidae NS-1 was named. It is noteworthy that I. fonsfrigidae NS-1 could metabolize multiple carbohydrates including xylan, alginate, starch, and lignin, and thereby produce diverse fermentation products such as hydrogen, lactate, butyrate, and ethanol. The expressions of the key genes responsible for carbohydrate degradation as well as the production of the above small molecular substrates when strain NS-1 cultured under different conditions, were further analyzed by transcriptomic methods. We thus predicted that part of the ecological role of Iocasia sp. is likely in the fermentation of products from the degradation of diverse carbohydrates to produce hydrogen as well as other small molecules, which are in turn utilized by other members of cold seep microbes.
format	article
author	Jing Zhang Jing Zhang Jing Zhang Jing Zhang Jing Zhang Yuechao Zhang Rui Liu Rui Liu Rui Liu Ruining Cai Ruining Cai Ruining Cai Ruining Cai Fanghua Liu Chaomin Sun Chaomin Sun Chaomin Sun
author_facet	Jing Zhang Jing Zhang Jing Zhang Jing Zhang Jing Zhang Yuechao Zhang Rui Liu Rui Liu Rui Liu Ruining Cai Ruining Cai Ruining Cai Ruining Cai Fanghua Liu Chaomin Sun Chaomin Sun Chaomin Sun
author_sort	Jing Zhang
title	Iocasia fonsfrigidae NS-1 gen. nov., sp. nov., a Novel Deep-Sea Bacterium Possessing Diverse Carbohydrate Metabolic Pathways
title_short	Iocasia fonsfrigidae NS-1 gen. nov., sp. nov., a Novel Deep-Sea Bacterium Possessing Diverse Carbohydrate Metabolic Pathways
title_full	Iocasia fonsfrigidae NS-1 gen. nov., sp. nov., a Novel Deep-Sea Bacterium Possessing Diverse Carbohydrate Metabolic Pathways
title_fullStr	Iocasia fonsfrigidae NS-1 gen. nov., sp. nov., a Novel Deep-Sea Bacterium Possessing Diverse Carbohydrate Metabolic Pathways
title_full_unstemmed	Iocasia fonsfrigidae NS-1 gen. nov., sp. nov., a Novel Deep-Sea Bacterium Possessing Diverse Carbohydrate Metabolic Pathways
title_sort	iocasia fonsfrigidae ns-1 gen. nov., sp. nov., a novel deep-sea bacterium possessing diverse carbohydrate metabolic pathways
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/97b993e21ab94bbc907c043ace0cd924
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Iocasia fonsfrigidae NS-1 gen. nov., sp. nov., a Novel Deep-Sea Bacterium Possessing Diverse Carbohydrate Metabolic Pathways

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