Sterol Composition Modulates the Response of <i>Saccharomyces cerevisiae</i> to Iron Deficiency

Iron is a vital micronutrient that functions as an essential cofactor in multiple biological processes, including oxygen transport, cellular respiration, and metabolic pathways, such as sterol biosynthesis. However, its low bioavailability at physiological pH frequently leads to nutritional iron def...

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Autores principales:	Tania Jordá, Nicolas Rozès, Sergi Puig
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	baker’s yeast <i>Saccharomyces cerevisiae</i> iron deficiency sterols ergosterol Upc2 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/97b9bc1e01594110b19637d7f8baf09f
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spelling	oai:doaj.org-article:97b9bc1e01594110b19637d7f8baf09f2021-11-25T18:05:34ZSterol Composition Modulates the Response of <i>Saccharomyces cerevisiae</i> to Iron Deficiency10.3390/jof71109012309-608Xhttps://doaj.org/article/97b9bc1e01594110b19637d7f8baf09f2021-10-01T00:00:00Zhttps://www.mdpi.com/2309-608X/7/11/901https://doaj.org/toc/2309-608XIron is a vital micronutrient that functions as an essential cofactor in multiple biological processes, including oxygen transport, cellular respiration, and metabolic pathways, such as sterol biosynthesis. However, its low bioavailability at physiological pH frequently leads to nutritional iron deficiency. The yeast <i>Saccharomyces cerevisiae</i> is extensively used to study iron and lipid metabolisms, as well as in multiple biotechnological applications. Despite iron being indispensable for yeast ergosterol biosynthesis and growth, little is known about their interconnections. Here, we used lipid composition analyses to determine that changes in the pattern of sterols impair the response to iron deprivation of yeast cells. Yeast mutants defective in ergosterol biosynthesis display defects in the transcriptional activation of the iron-acquisition machinery and growth defects in iron-depleted conditions. The transcriptional activation function of the iron-sensing Aft1 factor is interrupted due to its mislocalization to the vacuole. These data uncover novel links between iron and sterol metabolisms that need to be considered when producing yeast-derived foods or when treating fungal infections with drugs that target the ergosterol biosynthesis pathway.Tania JordáNicolas RozèsSergi PuigMDPI AGarticlebaker’s yeast<i>Saccharomyces cerevisiae</i>iron deficiencysterolsergosterolUpc2Biology (General)QH301-705.5ENJournal of Fungi, Vol 7, Iss 901, p 901 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	baker’s yeast <i>Saccharomyces cerevisiae</i> iron deficiency sterols ergosterol Upc2 Biology (General) QH301-705.5
spellingShingle	baker’s yeast <i>Saccharomyces cerevisiae</i> iron deficiency sterols ergosterol Upc2 Biology (General) QH301-705.5 Tania Jordá Nicolas Rozès Sergi Puig Sterol Composition Modulates the Response of <i>Saccharomyces cerevisiae</i> to Iron Deficiency
description	Iron is a vital micronutrient that functions as an essential cofactor in multiple biological processes, including oxygen transport, cellular respiration, and metabolic pathways, such as sterol biosynthesis. However, its low bioavailability at physiological pH frequently leads to nutritional iron deficiency. The yeast <i>Saccharomyces cerevisiae</i> is extensively used to study iron and lipid metabolisms, as well as in multiple biotechnological applications. Despite iron being indispensable for yeast ergosterol biosynthesis and growth, little is known about their interconnections. Here, we used lipid composition analyses to determine that changes in the pattern of sterols impair the response to iron deprivation of yeast cells. Yeast mutants defective in ergosterol biosynthesis display defects in the transcriptional activation of the iron-acquisition machinery and growth defects in iron-depleted conditions. The transcriptional activation function of the iron-sensing Aft1 factor is interrupted due to its mislocalization to the vacuole. These data uncover novel links between iron and sterol metabolisms that need to be considered when producing yeast-derived foods or when treating fungal infections with drugs that target the ergosterol biosynthesis pathway.
format	article
author	Tania Jordá Nicolas Rozès Sergi Puig
author_facet	Tania Jordá Nicolas Rozès Sergi Puig
author_sort	Tania Jordá
title	Sterol Composition Modulates the Response of <i>Saccharomyces cerevisiae</i> to Iron Deficiency
title_short	Sterol Composition Modulates the Response of <i>Saccharomyces cerevisiae</i> to Iron Deficiency
title_full	Sterol Composition Modulates the Response of <i>Saccharomyces cerevisiae</i> to Iron Deficiency
title_fullStr	Sterol Composition Modulates the Response of <i>Saccharomyces cerevisiae</i> to Iron Deficiency
title_full_unstemmed	Sterol Composition Modulates the Response of <i>Saccharomyces cerevisiae</i> to Iron Deficiency
title_sort	sterol composition modulates the response of <i>saccharomyces cerevisiae</i> to iron deficiency
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/97b9bc1e01594110b19637d7f8baf09f
work_keys_str_mv	AT taniajorda sterolcompositionmodulatestheresponseofisaccharomycescerevisiaeitoirondeficiency AT nicolasrozes sterolcompositionmodulatestheresponseofisaccharomycescerevisiaeitoirondeficiency AT sergipuig sterolcompositionmodulatestheresponseofisaccharomycescerevisiaeitoirondeficiency
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Sterol Composition Modulates the Response of <i>Saccharomyces cerevisiae</i> to Iron Deficiency

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