An In Vitro Model for the Ocular Surface and Tear Film System

Abstract Dry eye is a complicated ocular surface disease whose exact pathogenesis is not yet fully understood. For the therapeutic evaluation and pathogenesis study of dry eye, we established an in vitro three-dimensional (3D) coculture model for the ocular surface. It is composed of rabbit conjunct...

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Autores principales:	Qiaozhi Lu, Hongbo Yin, Michael P. Grant, Jennifer H. Elisseeff
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/97b9f8ff1b2d4853930753318411141f
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spelling	oai:doaj.org-article:97b9f8ff1b2d4853930753318411141f2021-12-02T15:05:00ZAn In Vitro Model for the Ocular Surface and Tear Film System10.1038/s41598-017-06369-82045-2322https://doaj.org/article/97b9f8ff1b2d4853930753318411141f2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06369-8https://doaj.org/toc/2045-2322Abstract Dry eye is a complicated ocular surface disease whose exact pathogenesis is not yet fully understood. For the therapeutic evaluation and pathogenesis study of dry eye, we established an in vitro three-dimensional (3D) coculture model for the ocular surface. It is composed of rabbit conjunctival epithelium and lacrimal gland cell spheroids, and recapitulates the aqueous and mucin layers of the tear film. We first investigated the culture conditions for both cell types to optimize their secretory functions, by employing goblet cell enrichment, air-lifting culture, and 3D spheroid formation techniques. The coculture of the two cell components leads to elevated secretion and higher expression of tear secretory markers. We also compared several coculture systems, and found that direct cell contact between the two cell types significantly increased tear secretion. Inflammation was induced to mimic dry eye disease in the coculture model system. Our results showed that the coculture system provides a more physiologically relevant therapeutic response compared to monocultures. Our work provides a complex 3D model as a recapitulation of the ocular surface and tear film system, which can be further developed as a model for dry eye disease and therapeutic evaluation.Qiaozhi LuHongbo YinMichael P. GrantJennifer H. ElisseeffNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-11 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Qiaozhi Lu Hongbo Yin Michael P. Grant Jennifer H. Elisseeff An In Vitro Model for the Ocular Surface and Tear Film System
description	Abstract Dry eye is a complicated ocular surface disease whose exact pathogenesis is not yet fully understood. For the therapeutic evaluation and pathogenesis study of dry eye, we established an in vitro three-dimensional (3D) coculture model for the ocular surface. It is composed of rabbit conjunctival epithelium and lacrimal gland cell spheroids, and recapitulates the aqueous and mucin layers of the tear film. We first investigated the culture conditions for both cell types to optimize their secretory functions, by employing goblet cell enrichment, air-lifting culture, and 3D spheroid formation techniques. The coculture of the two cell components leads to elevated secretion and higher expression of tear secretory markers. We also compared several coculture systems, and found that direct cell contact between the two cell types significantly increased tear secretion. Inflammation was induced to mimic dry eye disease in the coculture model system. Our results showed that the coculture system provides a more physiologically relevant therapeutic response compared to monocultures. Our work provides a complex 3D model as a recapitulation of the ocular surface and tear film system, which can be further developed as a model for dry eye disease and therapeutic evaluation.
format	article
author	Qiaozhi Lu Hongbo Yin Michael P. Grant Jennifer H. Elisseeff
author_facet	Qiaozhi Lu Hongbo Yin Michael P. Grant Jennifer H. Elisseeff
author_sort	Qiaozhi Lu
title	An In Vitro Model for the Ocular Surface and Tear Film System
title_short	An In Vitro Model for the Ocular Surface and Tear Film System
title_full	An In Vitro Model for the Ocular Surface and Tear Film System
title_fullStr	An In Vitro Model for the Ocular Surface and Tear Film System
title_full_unstemmed	An In Vitro Model for the Ocular Surface and Tear Film System
title_sort	in vitro model for the ocular surface and tear film system
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/97b9f8ff1b2d4853930753318411141f
work_keys_str_mv	AT qiaozhilu aninvitromodelfortheocularsurfaceandtearfilmsystem AT hongboyin aninvitromodelfortheocularsurfaceandtearfilmsystem AT michaelpgrant aninvitromodelfortheocularsurfaceandtearfilmsystem AT jenniferhelisseeff aninvitromodelfortheocularsurfaceandtearfilmsystem AT qiaozhilu invitromodelfortheocularsurfaceandtearfilmsystem AT hongboyin invitromodelfortheocularsurfaceandtearfilmsystem AT michaelpgrant invitromodelfortheocularsurfaceandtearfilmsystem AT jenniferhelisseeff invitromodelfortheocularsurfaceandtearfilmsystem
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An In Vitro Model for the Ocular Surface and Tear Film System

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