A Fluorescent Aptasensor Based on Assembled G-Quadruplex and Thioflavin T for the Detection of Biomarker VEGF165

VEGF165, a regulator of angiogenesis, has been widely used as a serum biomarker for a number of human diseases, including cancer, rheumatoid arthritis, bronchial asthma, and diabetic eye disease. The rapid, accurate, and convenient detection of VEGF165 is a crucial step in effective healthcare monit...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Xin Zheng, Shunxiang Gao, Jihong Wu, Xiaobo Hu
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	aptamer biomarker biosensor assembled G-quadruplex thioflavin T Biotechnology TP248.13-248.65
Acceso en línea:	https://doaj.org/article/97bd1314d2b443ccbd749abf4988dcd9
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:97bd1314d2b443ccbd749abf4988dcd9
record_format	dspace
spelling	oai:doaj.org-article:97bd1314d2b443ccbd749abf4988dcd92021-11-16T07:46:11ZA Fluorescent Aptasensor Based on Assembled G-Quadruplex and Thioflavin T for the Detection of Biomarker VEGF1652296-418510.3389/fbioe.2021.764123https://doaj.org/article/97bd1314d2b443ccbd749abf4988dcd92021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fbioe.2021.764123/fullhttps://doaj.org/toc/2296-4185VEGF165, a regulator of angiogenesis, has been widely used as a serum biomarker for a number of human diseases, including cancer, rheumatoid arthritis, bronchial asthma, and diabetic eye disease. The rapid, accurate, and convenient detection of VEGF165 is a crucial step in effective healthcare monitoring, disease diagnosis, and prognosis assessment. In this study, a fluorescent aptasensor based on an assembled G-quadruplex and the signal molecule ThT was developed for VEGF165 detection. First, G-rich DNA fragments were assembled at both ends of the anti-VEGF165 aptamer, and the B-DNA form was converted into a G-quadruplex structure aptamer (G4-Apt). Then, ThT was introduced, and the G-quadruplex significantly enhanced the fluorescence intensity of the bound ThT. When VEGF165 was present, the higher affinity of the aptamer to the target protein allowed the G4-Apt/VEGF165 complex to form and release ThT, which emitted only weak fluorescence in the free state. Therefore, the aptasensor exhibited a good linear detection window from 1.56 to 25 nM VEGF165, with a limit of detection of 0.138 nM. In addition, the aptasensor was applied to detect VEGF165 in clinical serum samples, showing good accuracy, reproducibility, and stability. These results indicate that our developed fluorescent aptasensor can potentially be a reliable, convenient, and cost-effective approach for the sensitive, specific, and rapid detection of the VEGF165 biomarker.Xin ZhengShunxiang GaoShunxiang GaoShunxiang GaoShunxiang GaoShunxiang GaoJihong WuJihong WuJihong WuJihong WuJihong WuXiaobo HuFrontiers Media S.A.articleaptamerbiomarkerbiosensorassembled G-quadruplexthioflavin TBiotechnologyTP248.13-248.65ENFrontiers in Bioengineering and Biotechnology, Vol 9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	aptamer biomarker biosensor assembled G-quadruplex thioflavin T Biotechnology TP248.13-248.65
spellingShingle	aptamer biomarker biosensor assembled G-quadruplex thioflavin T Biotechnology TP248.13-248.65 Xin Zheng Shunxiang Gao Shunxiang Gao Shunxiang Gao Shunxiang Gao Shunxiang Gao Jihong Wu Jihong Wu Jihong Wu Jihong Wu Jihong Wu Xiaobo Hu A Fluorescent Aptasensor Based on Assembled G-Quadruplex and Thioflavin T for the Detection of Biomarker VEGF165
description	VEGF165, a regulator of angiogenesis, has been widely used as a serum biomarker for a number of human diseases, including cancer, rheumatoid arthritis, bronchial asthma, and diabetic eye disease. The rapid, accurate, and convenient detection of VEGF165 is a crucial step in effective healthcare monitoring, disease diagnosis, and prognosis assessment. In this study, a fluorescent aptasensor based on an assembled G-quadruplex and the signal molecule ThT was developed for VEGF165 detection. First, G-rich DNA fragments were assembled at both ends of the anti-VEGF165 aptamer, and the B-DNA form was converted into a G-quadruplex structure aptamer (G4-Apt). Then, ThT was introduced, and the G-quadruplex significantly enhanced the fluorescence intensity of the bound ThT. When VEGF165 was present, the higher affinity of the aptamer to the target protein allowed the G4-Apt/VEGF165 complex to form and release ThT, which emitted only weak fluorescence in the free state. Therefore, the aptasensor exhibited a good linear detection window from 1.56 to 25 nM VEGF165, with a limit of detection of 0.138 nM. In addition, the aptasensor was applied to detect VEGF165 in clinical serum samples, showing good accuracy, reproducibility, and stability. These results indicate that our developed fluorescent aptasensor can potentially be a reliable, convenient, and cost-effective approach for the sensitive, specific, and rapid detection of the VEGF165 biomarker.
format	article
author	Xin Zheng Shunxiang Gao Shunxiang Gao Shunxiang Gao Shunxiang Gao Shunxiang Gao Jihong Wu Jihong Wu Jihong Wu Jihong Wu Jihong Wu Xiaobo Hu
author_facet	Xin Zheng Shunxiang Gao Shunxiang Gao Shunxiang Gao Shunxiang Gao Shunxiang Gao Jihong Wu Jihong Wu Jihong Wu Jihong Wu Jihong Wu Xiaobo Hu
author_sort	Xin Zheng
title	A Fluorescent Aptasensor Based on Assembled G-Quadruplex and Thioflavin T for the Detection of Biomarker VEGF165
title_short	A Fluorescent Aptasensor Based on Assembled G-Quadruplex and Thioflavin T for the Detection of Biomarker VEGF165
title_full	A Fluorescent Aptasensor Based on Assembled G-Quadruplex and Thioflavin T for the Detection of Biomarker VEGF165
title_fullStr	A Fluorescent Aptasensor Based on Assembled G-Quadruplex and Thioflavin T for the Detection of Biomarker VEGF165
title_full_unstemmed	A Fluorescent Aptasensor Based on Assembled G-Quadruplex and Thioflavin T for the Detection of Biomarker VEGF165
title_sort	fluorescent aptasensor based on assembled g-quadruplex and thioflavin t for the detection of biomarker vegf165
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/97bd1314d2b443ccbd749abf4988dcd9
work_keys_str_mv	AT xinzheng afluorescentaptasensorbasedonassembledgquadruplexandthioflavintforthedetectionofbiomarkervegf165 AT shunxianggao afluorescentaptasensorbasedonassembledgquadruplexandthioflavintforthedetectionofbiomarkervegf165 AT shunxianggao afluorescentaptasensorbasedonassembledgquadruplexandthioflavintforthedetectionofbiomarkervegf165 AT shunxianggao afluorescentaptasensorbasedonassembledgquadruplexandthioflavintforthedetectionofbiomarkervegf165 AT shunxianggao afluorescentaptasensorbasedonassembledgquadruplexandthioflavintforthedetectionofbiomarkervegf165 AT shunxianggao afluorescentaptasensorbasedonassembledgquadruplexandthioflavintforthedetectionofbiomarkervegf165 AT jihongwu afluorescentaptasensorbasedonassembledgquadruplexandthioflavintforthedetectionofbiomarkervegf165 AT jihongwu afluorescentaptasensorbasedonassembledgquadruplexandthioflavintforthedetectionofbiomarkervegf165 AT jihongwu afluorescentaptasensorbasedonassembledgquadruplexandthioflavintforthedetectionofbiomarkervegf165 AT jihongwu afluorescentaptasensorbasedonassembledgquadruplexandthioflavintforthedetectionofbiomarkervegf165 AT jihongwu afluorescentaptasensorbasedonassembledgquadruplexandthioflavintforthedetectionofbiomarkervegf165 AT xiaobohu afluorescentaptasensorbasedonassembledgquadruplexandthioflavintforthedetectionofbiomarkervegf165 AT xinzheng fluorescentaptasensorbasedonassembledgquadruplexandthioflavintforthedetectionofbiomarkervegf165 AT shunxianggao fluorescentaptasensorbasedonassembledgquadruplexandthioflavintforthedetectionofbiomarkervegf165 AT shunxianggao fluorescentaptasensorbasedonassembledgquadruplexandthioflavintforthedetectionofbiomarkervegf165 AT shunxianggao fluorescentaptasensorbasedonassembledgquadruplexandthioflavintforthedetectionofbiomarkervegf165 AT shunxianggao fluorescentaptasensorbasedonassembledgquadruplexandthioflavintforthedetectionofbiomarkervegf165 AT shunxianggao fluorescentaptasensorbasedonassembledgquadruplexandthioflavintforthedetectionofbiomarkervegf165 AT jihongwu fluorescentaptasensorbasedonassembledgquadruplexandthioflavintforthedetectionofbiomarkervegf165 AT jihongwu fluorescentaptasensorbasedonassembledgquadruplexandthioflavintforthedetectionofbiomarkervegf165 AT jihongwu fluorescentaptasensorbasedonassembledgquadruplexandthioflavintforthedetectionofbiomarkervegf165 AT jihongwu fluorescentaptasensorbasedonassembledgquadruplexandthioflavintforthedetectionofbiomarkervegf165 AT jihongwu fluorescentaptasensorbasedonassembledgquadruplexandthioflavintforthedetectionofbiomarkervegf165 AT xiaobohu fluorescentaptasensorbasedonassembledgquadruplexandthioflavintforthedetectionofbiomarkervegf165
_version_	1718426619516813312

A Fluorescent Aptasensor Based on Assembled G-Quadruplex and Thioflavin T for the Detection of Biomarker VEGF165

Ejemplares similares