Genome-wide fitness analyses of the foodborne pathogen Campylobacter jejuni in in vitro and in vivo models

Abstract Campylobacter is the most common cause of foodborne bacterial illness worldwide. Faecal contamination of meat, especially chicken, during processing represents a key route of transmission to humans. There is a lack of insight into the mechanisms driving C. jejuni growth and survival within...

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Autores principales:	Stefan P. de Vries, Srishti Gupta, Abiyad Baig, Elli Wright, Amy Wedley, Annette Nygaard Jensen, Lizeth LaCharme Lora, Suzanne Humphrey, Henrik Skovgård, Kareen Macleod, Elsa Pont, Dominika P. Wolanska, Joanna L’Heureux, Fredrick M. Mobegi, David G. E. Smith, Paul Everest, Aldert Zomer, Nicola Williams, Paul Wigley, Thomas Humphrey, Duncan J. Maskell, Andrew J. Grant
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/97bdaa5d3de04be88581fee3a9a3bd48
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spelling	oai:doaj.org-article:97bdaa5d3de04be88581fee3a9a3bd482021-12-02T11:40:22ZGenome-wide fitness analyses of the foodborne pathogen Campylobacter jejuni in in vitro and in vivo models10.1038/s41598-017-01133-42045-2322https://doaj.org/article/97bdaa5d3de04be88581fee3a9a3bd482017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01133-4https://doaj.org/toc/2045-2322Abstract Campylobacter is the most common cause of foodborne bacterial illness worldwide. Faecal contamination of meat, especially chicken, during processing represents a key route of transmission to humans. There is a lack of insight into the mechanisms driving C. jejuni growth and survival within hosts and the environment. Here, we report a detailed analysis of C. jejuni fitness across models reflecting stages in its life cycle. Transposon (Tn) gene-inactivation libraries were generated in three C. jejuni strains and the impact on fitness during chicken colonisation, survival in houseflies and under nutrient-rich and –poor conditions at 4 °C and infection of human gut epithelial cells was assessed by Tn-insertion site sequencing (Tn-seq). A total of 331 homologous gene clusters were essential for fitness during in vitro growth in three C. jejuni strains, revealing that a large part of its genome is dedicated to growth. We report novel C. jejuni factors essential throughout its life cycle. Importantly, we identified genes that fulfil important roles across multiple conditions. Our comprehensive screens showed which flagella elements are essential for growth and which are vital to the interaction with host organisms. Future efforts should focus on how to exploit this knowledge to effectively control infections caused by C. jejuni.Stefan P. de VriesSrishti GuptaAbiyad BaigElli WrightAmy WedleyAnnette Nygaard JensenLizeth LaCharme LoraSuzanne HumphreyHenrik SkovgårdKareen MacleodElsa PontDominika P. WolanskaJoanna L’HeureuxFredrick M. MobegiDavid G. E. SmithPaul EverestAldert ZomerNicola WilliamsPaul WigleyThomas HumphreyDuncan J. MaskellAndrew J. GrantNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-17 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Stefan P. de Vries Srishti Gupta Abiyad Baig Elli Wright Amy Wedley Annette Nygaard Jensen Lizeth LaCharme Lora Suzanne Humphrey Henrik Skovgård Kareen Macleod Elsa Pont Dominika P. Wolanska Joanna L’Heureux Fredrick M. Mobegi David G. E. Smith Paul Everest Aldert Zomer Nicola Williams Paul Wigley Thomas Humphrey Duncan J. Maskell Andrew J. Grant Genome-wide fitness analyses of the foodborne pathogen Campylobacter jejuni in in vitro and in vivo models
description	Abstract Campylobacter is the most common cause of foodborne bacterial illness worldwide. Faecal contamination of meat, especially chicken, during processing represents a key route of transmission to humans. There is a lack of insight into the mechanisms driving C. jejuni growth and survival within hosts and the environment. Here, we report a detailed analysis of C. jejuni fitness across models reflecting stages in its life cycle. Transposon (Tn) gene-inactivation libraries were generated in three C. jejuni strains and the impact on fitness during chicken colonisation, survival in houseflies and under nutrient-rich and –poor conditions at 4 °C and infection of human gut epithelial cells was assessed by Tn-insertion site sequencing (Tn-seq). A total of 331 homologous gene clusters were essential for fitness during in vitro growth in three C. jejuni strains, revealing that a large part of its genome is dedicated to growth. We report novel C. jejuni factors essential throughout its life cycle. Importantly, we identified genes that fulfil important roles across multiple conditions. Our comprehensive screens showed which flagella elements are essential for growth and which are vital to the interaction with host organisms. Future efforts should focus on how to exploit this knowledge to effectively control infections caused by C. jejuni.
format	article
author	Stefan P. de Vries Srishti Gupta Abiyad Baig Elli Wright Amy Wedley Annette Nygaard Jensen Lizeth LaCharme Lora Suzanne Humphrey Henrik Skovgård Kareen Macleod Elsa Pont Dominika P. Wolanska Joanna L’Heureux Fredrick M. Mobegi David G. E. Smith Paul Everest Aldert Zomer Nicola Williams Paul Wigley Thomas Humphrey Duncan J. Maskell Andrew J. Grant
author_facet	Stefan P. de Vries Srishti Gupta Abiyad Baig Elli Wright Amy Wedley Annette Nygaard Jensen Lizeth LaCharme Lora Suzanne Humphrey Henrik Skovgård Kareen Macleod Elsa Pont Dominika P. Wolanska Joanna L’Heureux Fredrick M. Mobegi David G. E. Smith Paul Everest Aldert Zomer Nicola Williams Paul Wigley Thomas Humphrey Duncan J. Maskell Andrew J. Grant
author_sort	Stefan P. de Vries
title	Genome-wide fitness analyses of the foodborne pathogen Campylobacter jejuni in in vitro and in vivo models
title_short	Genome-wide fitness analyses of the foodborne pathogen Campylobacter jejuni in in vitro and in vivo models
title_full	Genome-wide fitness analyses of the foodborne pathogen Campylobacter jejuni in in vitro and in vivo models
title_fullStr	Genome-wide fitness analyses of the foodborne pathogen Campylobacter jejuni in in vitro and in vivo models
title_full_unstemmed	Genome-wide fitness analyses of the foodborne pathogen Campylobacter jejuni in in vitro and in vivo models
title_sort	genome-wide fitness analyses of the foodborne pathogen campylobacter jejuni in in vitro and in vivo models
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/97bdaa5d3de04be88581fee3a9a3bd48
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Genome-wide fitness analyses of the foodborne pathogen Campylobacter jejuni in in vitro and in vivo models

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