Two Laboratory Activities Using Conventional or Real-Time PCR to Simulate Pathogenic <italic toggle="yes">E. coli</italic> Detection

In these lab activities, students perform conventional PCR and real-time PCR to simulate pathogenic E. coli detection. The labs were designed to complement a previously published virtual PCR classroom activity in which students are asked to design a PCR-based diagnostic test for a pathogenic strain...

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Auteur principal:	Joanna R. Klein
Format:	article
Langue:	EN
Publié:	American Society for Microbiology 2014
Sujets:	Special aspects of education LC8-6691 Biology (General) QH301-705.5
Accès en ligne:	https://doaj.org/article/97be933d8fa64694bea759ad8927bc72
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spelling	oai:doaj.org-article:97be933d8fa64694bea759ad8927bc722021-11-15T15:03:37ZTwo Laboratory Activities Using Conventional or Real-Time PCR to Simulate Pathogenic <italic toggle="yes">E. coli</italic> Detection10.1128/jmbe.v15i1.6651935-78851935-7877https://doaj.org/article/97be933d8fa64694bea759ad8927bc722014-05-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/jmbe.v15i1.665https://doaj.org/toc/1935-7877https://doaj.org/toc/1935-7885In these lab activities, students perform conventional PCR and real-time PCR to simulate pathogenic E. coli detection. The labs were designed to complement a previously published virtual PCR classroom activity in which students are asked to design a PCR-based diagnostic test for a pathogenic strain of E. coli. In the virtual PCR activity, students use bioinformatics to discover that the Shiga toxin genes (stx1 and stx2) are unique to the Enterohemorrhagic E. coli (EHEC) strain O157:H7. Thus they come to the conclusion that doing PCR with primers designed for shiga toxin should be able to differentiate O157:H7 from other strains of E. coli. In the lab activity described here, students actually perform the PCR assay. Performing PCR enhanced student understanding of the technique beyond what was accomplished through the virtual PCR classroom activity and is recommended as an addition to the case study.Joanna R. KleinAmerican Society for MicrobiologyarticleSpecial aspects of educationLC8-6691Biology (General)QH301-705.5ENJournal of Microbiology & Biology Education, Vol 15, Iss 1, Pp 51-52 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Special aspects of education LC8-6691 Biology (General) QH301-705.5
spellingShingle	Special aspects of education LC8-6691 Biology (General) QH301-705.5 Joanna R. Klein Two Laboratory Activities Using Conventional or Real-Time PCR to Simulate Pathogenic <italic toggle="yes">E. coli</italic> Detection
description	In these lab activities, students perform conventional PCR and real-time PCR to simulate pathogenic E. coli detection. The labs were designed to complement a previously published virtual PCR classroom activity in which students are asked to design a PCR-based diagnostic test for a pathogenic strain of E. coli. In the virtual PCR activity, students use bioinformatics to discover that the Shiga toxin genes (stx1 and stx2) are unique to the Enterohemorrhagic E. coli (EHEC) strain O157:H7. Thus they come to the conclusion that doing PCR with primers designed for shiga toxin should be able to differentiate O157:H7 from other strains of E. coli. In the lab activity described here, students actually perform the PCR assay. Performing PCR enhanced student understanding of the technique beyond what was accomplished through the virtual PCR classroom activity and is recommended as an addition to the case study.
format	article
author	Joanna R. Klein
author_facet	Joanna R. Klein
author_sort	Joanna R. Klein
title	Two Laboratory Activities Using Conventional or Real-Time PCR to Simulate Pathogenic <italic toggle="yes">E. coli</italic> Detection
title_short	Two Laboratory Activities Using Conventional or Real-Time PCR to Simulate Pathogenic <italic toggle="yes">E. coli</italic> Detection
title_full	Two Laboratory Activities Using Conventional or Real-Time PCR to Simulate Pathogenic <italic toggle="yes">E. coli</italic> Detection
title_fullStr	Two Laboratory Activities Using Conventional or Real-Time PCR to Simulate Pathogenic <italic toggle="yes">E. coli</italic> Detection
title_full_unstemmed	Two Laboratory Activities Using Conventional or Real-Time PCR to Simulate Pathogenic <italic toggle="yes">E. coli</italic> Detection
title_sort	two laboratory activities using conventional or real-time pcr to simulate pathogenic <italic toggle="yes">e. coli</italic> detection
publisher	American Society for Microbiology
publishDate	2014
url	https://doaj.org/article/97be933d8fa64694bea759ad8927bc72
work_keys_str_mv	AT joannarklein twolaboratoryactivitiesusingconventionalorrealtimepcrtosimulatepathogenicitalictoggleyesecoliitalicdetection
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Two Laboratory Activities Using Conventional or Real-Time PCR to Simulate Pathogenic <italic toggle="yes">E. coli</italic> Detection

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