The parasexual cycle in Candida albicans provides an alternative pathway to meiosis for the formation of recombinant strains.

Candida albicans has an elaborate, yet efficient, mating system that promotes conjugation between diploid a and alpha strains. The product of mating is a tetraploid a/alpha cell that must undergo a reductional division to return to the diploid state. Despite the presence of several "meiosis-spe...

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Autores principales:	Anja Forche, Kevin Alby, Dana Schaefer, Alexander D Johnson, Judith Berman, Richard J Bennett
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2008
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/97c39029db9c40e3b4599c338a522ac2
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spelling	oai:doaj.org-article:97c39029db9c40e3b4599c338a522ac22021-11-25T05:33:22ZThe parasexual cycle in Candida albicans provides an alternative pathway to meiosis for the formation of recombinant strains.1544-91731545-788510.1371/journal.pbio.0060110https://doaj.org/article/97c39029db9c40e3b4599c338a522ac22008-05-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/18462019/?tool=EBIhttps://doaj.org/toc/1544-9173https://doaj.org/toc/1545-7885Candida albicans has an elaborate, yet efficient, mating system that promotes conjugation between diploid a and alpha strains. The product of mating is a tetraploid a/alpha cell that must undergo a reductional division to return to the diploid state. Despite the presence of several "meiosis-specific" genes in the C. albicans genome, a meiotic program has not been observed. Instead, tetraploid products of mating can be induced to undergo efficient, random chromosome loss, often producing strains that are diploid, or close to diploid, in ploidy. Using SNP and comparative genome hybridization arrays we have now analyzed the genotypes of products from the C. albicans parasexual cycle. We show that the parasexual cycle generates progeny strains with shuffled combinations of the eight C. albicans chromosomes. In addition, several isolates had undergone extensive genetic recombination between homologous chromosomes, including multiple gene conversion events. Progeny strains exhibited altered colony morphologies on laboratory media, demonstrating that the parasexual cycle generates phenotypic variants of C. albicans. In several fungi, including Saccharomyces cerevisiae and Schizosaccharomyces pombe, the conserved Spo11 protein is integral to meiotic recombination, where it is required for the formation of DNA double-strand breaks. We show that deletion of SPO11 prevented genetic recombination between homologous chromosomes during the C. albicans parasexual cycle. These findings suggest that at least one meiosis-specific gene has been re-programmed to mediate genetic recombination during the alternative parasexual life cycle of C. albicans. We discuss, in light of the long association of C. albicans with warm-blooded animals, the potential advantages of a parasexual cycle over a conventional sexual cycle.Anja ForcheKevin AlbyDana SchaeferAlexander D JohnsonJudith BermanRichard J BennettPublic Library of Science (PLoS)articleBiology (General)QH301-705.5ENPLoS Biology, Vol 6, Iss 5, p e110 (2008)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Anja Forche Kevin Alby Dana Schaefer Alexander D Johnson Judith Berman Richard J Bennett The parasexual cycle in Candida albicans provides an alternative pathway to meiosis for the formation of recombinant strains.
description	Candida albicans has an elaborate, yet efficient, mating system that promotes conjugation between diploid a and alpha strains. The product of mating is a tetraploid a/alpha cell that must undergo a reductional division to return to the diploid state. Despite the presence of several "meiosis-specific" genes in the C. albicans genome, a meiotic program has not been observed. Instead, tetraploid products of mating can be induced to undergo efficient, random chromosome loss, often producing strains that are diploid, or close to diploid, in ploidy. Using SNP and comparative genome hybridization arrays we have now analyzed the genotypes of products from the C. albicans parasexual cycle. We show that the parasexual cycle generates progeny strains with shuffled combinations of the eight C. albicans chromosomes. In addition, several isolates had undergone extensive genetic recombination between homologous chromosomes, including multiple gene conversion events. Progeny strains exhibited altered colony morphologies on laboratory media, demonstrating that the parasexual cycle generates phenotypic variants of C. albicans. In several fungi, including Saccharomyces cerevisiae and Schizosaccharomyces pombe, the conserved Spo11 protein is integral to meiotic recombination, where it is required for the formation of DNA double-strand breaks. We show that deletion of SPO11 prevented genetic recombination between homologous chromosomes during the C. albicans parasexual cycle. These findings suggest that at least one meiosis-specific gene has been re-programmed to mediate genetic recombination during the alternative parasexual life cycle of C. albicans. We discuss, in light of the long association of C. albicans with warm-blooded animals, the potential advantages of a parasexual cycle over a conventional sexual cycle.
format	article
author	Anja Forche Kevin Alby Dana Schaefer Alexander D Johnson Judith Berman Richard J Bennett
author_facet	Anja Forche Kevin Alby Dana Schaefer Alexander D Johnson Judith Berman Richard J Bennett
author_sort	Anja Forche
title	The parasexual cycle in Candida albicans provides an alternative pathway to meiosis for the formation of recombinant strains.
title_short	The parasexual cycle in Candida albicans provides an alternative pathway to meiosis for the formation of recombinant strains.
title_full	The parasexual cycle in Candida albicans provides an alternative pathway to meiosis for the formation of recombinant strains.
title_fullStr	The parasexual cycle in Candida albicans provides an alternative pathway to meiosis for the formation of recombinant strains.
title_full_unstemmed	The parasexual cycle in Candida albicans provides an alternative pathway to meiosis for the formation of recombinant strains.
title_sort	parasexual cycle in candida albicans provides an alternative pathway to meiosis for the formation of recombinant strains.
publisher	Public Library of Science (PLoS)
publishDate	2008
url	https://doaj.org/article/97c39029db9c40e3b4599c338a522ac2
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The parasexual cycle in Candida albicans provides an alternative pathway to meiosis for the formation of recombinant strains.

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