Short Communication: Insulin-like growth factor 1 (IGF-1) gene polymorphisms in Madura karapan and beef cattle breed

Abstract. Perwitasari D, Ummah RI, Nurjannah RA, Farajallah A. 2019. Short Communication: Insulin-like growth factor 1 (IGF-1) gene polymorphisms in Madura karapan and beef cattle breed. Biodiversitas 20: 3070-3074. Madura cattle breed is one of the four existing indigenous cattle breeds (Aceh, Pesi...

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Autores principales:	Dyah Perwitasari-Farajallah, ROSE IDA UMMAH, REVA ADZANI NURJANNAH, ACHMAD FARAJALLAH
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	igf-1, intron 4, madura cattle breed, point of mutation Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/97c7c54a31174c7ca1ef45de07eb3f99
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spelling	oai:doaj.org-article:97c7c54a31174c7ca1ef45de07eb3f992021-11-21T21:54:57ZShort Communication: Insulin-like growth factor 1 (IGF-1) gene polymorphisms in Madura karapan and beef cattle breed1412-033X2085-472210.13057/biodiv/d201041https://doaj.org/article/97c7c54a31174c7ca1ef45de07eb3f992019-09-01T00:00:00Zhttps://smujo.id/biodiv/article/view/4260https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Perwitasari D, Ummah RI, Nurjannah RA, Farajallah A. 2019. Short Communication: Insulin-like growth factor 1 (IGF-1) gene polymorphisms in Madura karapan and beef cattle breed. Biodiversitas 20: 3070-3074. Madura cattle breed is one of the four existing indigenous cattle breeds (Aceh, Pesisir, Madura, and Bali) in Indonesia. This Indonesian breed derived from crossbreeding between Bos indicus (zebu) and Bos javanicus (banteng). Cattle raising in Madura focused on three purposes i.e. bull race competition (karapan), beauty contest (sonok) and meat production. Insulin-like Growth Factor 1 (somatomedin-IGF-1) is a protein that plays a key role in cell differentiation, embryogenesis, growth, and regulation of metabolism. In cattle, the IGF-1 gene was localized on chromosome 5. Due to their role in the regulation of cell proliferation and animal growth, the IGF-1 and its gene are considered as candidate markers for growth rate and meat production traits. This research aimed to determine nucleotide variations in the IGF-1 gene among Madura karapan and beef cattle. PCR amplification of IGF-1 gene was conducted using a pair of primers. Amplicon was sent to commercial DNA sequencing company (1st Base) to perform DNA sequencing analysis. The size of IGF-1 gene was approximately 762 bp consisted of intron 3, exon 4, and intron 4. Sequence data were analyzed using MEGA 7 to identify the presence of nucleotide variation. Bos indicus (NC032654) was used as a reference sequence. The results showed no polymorphism was observed in intron 3 and exon 4 of Madura karapan and beef cattle breed. We determined G491A point mutation in intron 4. In conclusion, IGF-1 could not be able to be used as a genetic marker to differentiate karapan and beef cattle and furthermore morphological characteristics still can be applied as a standard for selection.Dyah Perwitasari-FarajallahROSE IDA UMMAHREVA ADZANI NURJANNAHACHMAD FARAJALLAHMBI & UNS Soloarticleigf-1, intron 4, madura cattle breed, point of mutationBiology (General)QH301-705.5ENBiodiversitas, Vol 20, Iss 10 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	igf-1, intron 4, madura cattle breed, point of mutation Biology (General) QH301-705.5
spellingShingle	igf-1, intron 4, madura cattle breed, point of mutation Biology (General) QH301-705.5 Dyah Perwitasari-Farajallah ROSE IDA UMMAH REVA ADZANI NURJANNAH ACHMAD FARAJALLAH Short Communication: Insulin-like growth factor 1 (IGF-1) gene polymorphisms in Madura karapan and beef cattle breed
description	Abstract. Perwitasari D, Ummah RI, Nurjannah RA, Farajallah A. 2019. Short Communication: Insulin-like growth factor 1 (IGF-1) gene polymorphisms in Madura karapan and beef cattle breed. Biodiversitas 20: 3070-3074. Madura cattle breed is one of the four existing indigenous cattle breeds (Aceh, Pesisir, Madura, and Bali) in Indonesia. This Indonesian breed derived from crossbreeding between Bos indicus (zebu) and Bos javanicus (banteng). Cattle raising in Madura focused on three purposes i.e. bull race competition (karapan), beauty contest (sonok) and meat production. Insulin-like Growth Factor 1 (somatomedin-IGF-1) is a protein that plays a key role in cell differentiation, embryogenesis, growth, and regulation of metabolism. In cattle, the IGF-1 gene was localized on chromosome 5. Due to their role in the regulation of cell proliferation and animal growth, the IGF-1 and its gene are considered as candidate markers for growth rate and meat production traits. This research aimed to determine nucleotide variations in the IGF-1 gene among Madura karapan and beef cattle. PCR amplification of IGF-1 gene was conducted using a pair of primers. Amplicon was sent to commercial DNA sequencing company (1st Base) to perform DNA sequencing analysis. The size of IGF-1 gene was approximately 762 bp consisted of intron 3, exon 4, and intron 4. Sequence data were analyzed using MEGA 7 to identify the presence of nucleotide variation. Bos indicus (NC032654) was used as a reference sequence. The results showed no polymorphism was observed in intron 3 and exon 4 of Madura karapan and beef cattle breed. We determined G491A point mutation in intron 4. In conclusion, IGF-1 could not be able to be used as a genetic marker to differentiate karapan and beef cattle and furthermore morphological characteristics still can be applied as a standard for selection.
format	article
author	Dyah Perwitasari-Farajallah ROSE IDA UMMAH REVA ADZANI NURJANNAH ACHMAD FARAJALLAH
author_facet	Dyah Perwitasari-Farajallah ROSE IDA UMMAH REVA ADZANI NURJANNAH ACHMAD FARAJALLAH
author_sort	Dyah Perwitasari-Farajallah
title	Short Communication: Insulin-like growth factor 1 (IGF-1) gene polymorphisms in Madura karapan and beef cattle breed
title_short	Short Communication: Insulin-like growth factor 1 (IGF-1) gene polymorphisms in Madura karapan and beef cattle breed
title_full	Short Communication: Insulin-like growth factor 1 (IGF-1) gene polymorphisms in Madura karapan and beef cattle breed
title_fullStr	Short Communication: Insulin-like growth factor 1 (IGF-1) gene polymorphisms in Madura karapan and beef cattle breed
title_full_unstemmed	Short Communication: Insulin-like growth factor 1 (IGF-1) gene polymorphisms in Madura karapan and beef cattle breed
title_sort	short communication: insulin-like growth factor 1 (igf-1) gene polymorphisms in madura karapan and beef cattle breed
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/97c7c54a31174c7ca1ef45de07eb3f99
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Short Communication: Insulin-like growth factor 1 (IGF-1) gene polymorphisms in Madura karapan and beef cattle breed

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