Ultra-sensitive method based on time-resolved fluorescence immunoassay for detection of sulfamethazine in raw milk

A novel lateral flow assay (LFA) was developed by introducing Eu (III)-doped polystyrene nanoparticles (EuNPs) for rapid and ultra-sensitive detection of sulfamethazine (SM2) in raw milk. The limit of detection and linear range of the proposed method were 0.0045 and 0.05–10 ng/mL, respectively. The...

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Autores principales:	Song Hu, Danhong Li, Zhen Huang, Keyu Xing, Yuan Chen, Juan Peng, Weihua Lai
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2018
Materias:	lateral flow assay eu (iii)-doped polystyrene nanoparticle sulfamethazine raw milk Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/97cb3c1bcef34be4883115f675e05342
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spelling	oai:doaj.org-article:97cb3c1bcef34be4883115f675e053422021-11-26T11:19:47ZUltra-sensitive method based on time-resolved fluorescence immunoassay for detection of sulfamethazine in raw milk0954-01051465-344310.1080/09540105.2018.1520816https://doaj.org/article/97cb3c1bcef34be4883115f675e053422018-01-01T00:00:00Zhttp://dx.doi.org/10.1080/09540105.2018.1520816https://doaj.org/toc/0954-0105https://doaj.org/toc/1465-3443A novel lateral flow assay (LFA) was developed by introducing Eu (III)-doped polystyrene nanoparticles (EuNPs) for rapid and ultra-sensitive detection of sulfamethazine (SM2) in raw milk. The limit of detection and linear range of the proposed method were 0.0045 and 0.05–10 ng/mL, respectively. The recovery of LFA for the detection of SM2 in raw milk was 96.1–108.2%. The proposed LFA provides a rapid and convenient strategy for fast and ultra-sensitive screening of SM2 in raw milk. EuNP-LFA may be a remarkable method for the detection of other targets at low concentrations to ensure food safety.Song HuDanhong LiZhen HuangKeyu XingYuan ChenJuan PengWeihua LaiTaylor & Francis Grouparticlelateral flow assayeu (iii)-doped polystyrene nanoparticlesulfamethazineraw milkAgriculture (General)S1-972Immunologic diseases. AllergyRC581-607ENFood and Agricultural Immunology, Vol 29, Iss 1, Pp 1137-1149 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	lateral flow assay eu (iii)-doped polystyrene nanoparticle sulfamethazine raw milk Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
spellingShingle	lateral flow assay eu (iii)-doped polystyrene nanoparticle sulfamethazine raw milk Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 Song Hu Danhong Li Zhen Huang Keyu Xing Yuan Chen Juan Peng Weihua Lai Ultra-sensitive method based on time-resolved fluorescence immunoassay for detection of sulfamethazine in raw milk
description	A novel lateral flow assay (LFA) was developed by introducing Eu (III)-doped polystyrene nanoparticles (EuNPs) for rapid and ultra-sensitive detection of sulfamethazine (SM2) in raw milk. The limit of detection and linear range of the proposed method were 0.0045 and 0.05–10 ng/mL, respectively. The recovery of LFA for the detection of SM2 in raw milk was 96.1–108.2%. The proposed LFA provides a rapid and convenient strategy for fast and ultra-sensitive screening of SM2 in raw milk. EuNP-LFA may be a remarkable method for the detection of other targets at low concentrations to ensure food safety.
format	article
author	Song Hu Danhong Li Zhen Huang Keyu Xing Yuan Chen Juan Peng Weihua Lai
author_facet	Song Hu Danhong Li Zhen Huang Keyu Xing Yuan Chen Juan Peng Weihua Lai
author_sort	Song Hu
title	Ultra-sensitive method based on time-resolved fluorescence immunoassay for detection of sulfamethazine in raw milk
title_short	Ultra-sensitive method based on time-resolved fluorescence immunoassay for detection of sulfamethazine in raw milk
title_full	Ultra-sensitive method based on time-resolved fluorescence immunoassay for detection of sulfamethazine in raw milk
title_fullStr	Ultra-sensitive method based on time-resolved fluorescence immunoassay for detection of sulfamethazine in raw milk
title_full_unstemmed	Ultra-sensitive method based on time-resolved fluorescence immunoassay for detection of sulfamethazine in raw milk
title_sort	ultra-sensitive method based on time-resolved fluorescence immunoassay for detection of sulfamethazine in raw milk
publisher	Taylor & Francis Group
publishDate	2018
url	https://doaj.org/article/97cb3c1bcef34be4883115f675e05342
work_keys_str_mv	AT songhu ultrasensitivemethodbasedontimeresolvedfluorescenceimmunoassayfordetectionofsulfamethazineinrawmilk AT danhongli ultrasensitivemethodbasedontimeresolvedfluorescenceimmunoassayfordetectionofsulfamethazineinrawmilk AT zhenhuang ultrasensitivemethodbasedontimeresolvedfluorescenceimmunoassayfordetectionofsulfamethazineinrawmilk AT keyuxing ultrasensitivemethodbasedontimeresolvedfluorescenceimmunoassayfordetectionofsulfamethazineinrawmilk AT yuanchen ultrasensitivemethodbasedontimeresolvedfluorescenceimmunoassayfordetectionofsulfamethazineinrawmilk AT juanpeng ultrasensitivemethodbasedontimeresolvedfluorescenceimmunoassayfordetectionofsulfamethazineinrawmilk AT weihualai ultrasensitivemethodbasedontimeresolvedfluorescenceimmunoassayfordetectionofsulfamethazineinrawmilk
_version_	1718409548793905152

Ultra-sensitive method based on time-resolved fluorescence immunoassay for detection of sulfamethazine in raw milk

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