$Quantifying the hydroxyapatite orientation near the ossification front in a piglet femoral condyle using X-ray diffraction tensor tomography$
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Quantifying the hydroxyapatite orientation near the ossification front in a piglet femoral condyle using X-ray diffraction tensor tomography

Abstract While a detailed knowledge of the hierarchical structure and morphology of the extracellular matrix is considered crucial for understanding the physiological and mechanical properties of bone and cartilage, the orientation of collagen fibres and carbonated hydroxyapatite (HA) crystallites r...

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Autores principales:	Fredrik K. Mürer, Basab Chattopadhyay, Aldritt Scaria Madathiparambil, Kim Robert Tekseth, Marco Di Michiel, Marianne Liebi, Magnus B. Lilledahl, Kristin Olstad, Dag W. Breiby
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/97d1c124c340415085c4c0cc23654dbd
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spelling	oai:doaj.org-article:97d1c124c340415085c4c0cc23654dbd2021-12-02T13:24:25ZQuantifying the hydroxyapatite orientation near the ossification front in a piglet femoral condyle using X-ray diffraction tensor tomography10.1038/s41598-020-80615-42045-2322https://doaj.org/article/97d1c124c340415085c4c0cc23654dbd2021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-80615-4https://doaj.org/toc/2045-2322Abstract While a detailed knowledge of the hierarchical structure and morphology of the extracellular matrix is considered crucial for understanding the physiological and mechanical properties of bone and cartilage, the orientation of collagen fibres and carbonated hydroxyapatite (HA) crystallites remains a debated topic. Conventional microscopy techniques for orientational imaging require destructive sample sectioning, which both precludes further studies of the intact sample and potentially changes the microstructure. In this work, we use X-ray diffraction tensor tomography to image non-destructively in 3D the HA orientation in a medial femoral condyle of a piglet. By exploiting the anisotropic HA diffraction signal, 3D maps showing systematic local variations of the HA crystallite orientation in the growing subchondral bone and in the adjacent mineralized growth cartilage are obtained. Orientation maps of HA crystallites over a large field of view (~ 3 × 3 × 3 mm3) close to the ossification (bone-growth) front are compared with high-resolution X-ray propagation phase-contrast computed tomography images. The HA crystallites are found to predominantly orient with their crystallite c-axis directed towards the ossification front. Distinct patterns of HA preferred orientation are found in the vicinity of cartilage canals protruding from the subchondral bone. The demonstrated ability of retrieving 3D orientation maps of bone-cartilage structures is expected to give a better understanding of the physiological properties of bones, including their propensity for bone-cartilage diseases.Fredrik K. MürerBasab ChattopadhyayAldritt Scaria MadathiparambilKim Robert TeksethMarco Di MichielMarianne LiebiMagnus B. LilledahlKristin OlstadDag W. BreibyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fredrik K. Mürer Basab Chattopadhyay Aldritt Scaria Madathiparambil Kim Robert Tekseth Marco Di Michiel Marianne Liebi Magnus B. Lilledahl Kristin Olstad Dag W. Breiby Quantifying the hydroxyapatite orientation near the ossification front in a piglet femoral condyle using X-ray diffraction tensor tomography
description	Abstract While a detailed knowledge of the hierarchical structure and morphology of the extracellular matrix is considered crucial for understanding the physiological and mechanical properties of bone and cartilage, the orientation of collagen fibres and carbonated hydroxyapatite (HA) crystallites remains a debated topic. Conventional microscopy techniques for orientational imaging require destructive sample sectioning, which both precludes further studies of the intact sample and potentially changes the microstructure. In this work, we use X-ray diffraction tensor tomography to image non-destructively in 3D the HA orientation in a medial femoral condyle of a piglet. By exploiting the anisotropic HA diffraction signal, 3D maps showing systematic local variations of the HA crystallite orientation in the growing subchondral bone and in the adjacent mineralized growth cartilage are obtained. Orientation maps of HA crystallites over a large field of view (~ 3 × 3 × 3 mm3) close to the ossification (bone-growth) front are compared with high-resolution X-ray propagation phase-contrast computed tomography images. The HA crystallites are found to predominantly orient with their crystallite c-axis directed towards the ossification front. Distinct patterns of HA preferred orientation are found in the vicinity of cartilage canals protruding from the subchondral bone. The demonstrated ability of retrieving 3D orientation maps of bone-cartilage structures is expected to give a better understanding of the physiological properties of bones, including their propensity for bone-cartilage diseases.
format	article
author	Fredrik K. Mürer Basab Chattopadhyay Aldritt Scaria Madathiparambil Kim Robert Tekseth Marco Di Michiel Marianne Liebi Magnus B. Lilledahl Kristin Olstad Dag W. Breiby
author_facet	Fredrik K. Mürer Basab Chattopadhyay Aldritt Scaria Madathiparambil Kim Robert Tekseth Marco Di Michiel Marianne Liebi Magnus B. Lilledahl Kristin Olstad Dag W. Breiby
author_sort	Fredrik K. Mürer
title	Quantifying the hydroxyapatite orientation near the ossification front in a piglet femoral condyle using X-ray diffraction tensor tomography
title_short	Quantifying the hydroxyapatite orientation near the ossification front in a piglet femoral condyle using X-ray diffraction tensor tomography
title_full	Quantifying the hydroxyapatite orientation near the ossification front in a piglet femoral condyle using X-ray diffraction tensor tomography
title_fullStr	Quantifying the hydroxyapatite orientation near the ossification front in a piglet femoral condyle using X-ray diffraction tensor tomography
title_full_unstemmed	Quantifying the hydroxyapatite orientation near the ossification front in a piglet femoral condyle using X-ray diffraction tensor tomography
title_sort	quantifying the hydroxyapatite orientation near the ossification front in a piglet femoral condyle using x-ray diffraction tensor tomography
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/97d1c124c340415085c4c0cc23654dbd
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Quantifying the hydroxyapatite orientation near the ossification front in a piglet femoral condyle using X-ray diffraction tensor tomography

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