Role of AmpR in the High Expression of the Plasmid-Encoded AmpC β-Lactamase CFE-1

ABSTRACT CFE-1 is a unique plasmid-encoded AmpC β-lactamase with the regulator gene ampR. It imparts high resistance to most cephalosporins with constitutive high-level β-lactamase activity. Here, the β-lactamase activities and expression levels of ampC with or without ampR were investigated. Result...

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Autores principales:	Ryuichi Nakano, Akiyo Nakano, Hisakazu Yano, Ryoichi Okamoto
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2017
Materias:	AmpR plasmid-encoded AmpC β-lactamase antibiotic resistance regulation Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/97d56aac9fb84060bedb3085d6093c89
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spelling	oai:doaj.org-article:97d56aac9fb84060bedb3085d6093c892021-11-15T15:22:05ZRole of AmpR in the High Expression of the Plasmid-Encoded AmpC β-Lactamase CFE-110.1128/mSphere.00192-172379-5042https://doaj.org/article/97d56aac9fb84060bedb3085d6093c892017-08-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00192-17https://doaj.org/toc/2379-5042ABSTRACT CFE-1 is a unique plasmid-encoded AmpC β-lactamase with the regulator gene ampR. It imparts high resistance to most cephalosporins with constitutive high-level β-lactamase activity. Here, the β-lactamase activities and expression levels of ampC with or without ampR were investigated. Results suggested that the resistance of CFE-1 to cephalosporins is caused by a substitution in AmpR, in which the Asp at position 135 is modified to Ala to allow the constitutive high-level expression (derepression) of ampC.Ryuichi NakanoAkiyo NakanoHisakazu YanoRyoichi OkamotoAmerican Society for MicrobiologyarticleAmpRplasmid-encoded AmpC β-lactamaseantibiotic resistanceregulationMicrobiologyQR1-502ENmSphere, Vol 2, Iss 4 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	AmpR plasmid-encoded AmpC β-lactamase antibiotic resistance regulation Microbiology QR1-502
spellingShingle	AmpR plasmid-encoded AmpC β-lactamase antibiotic resistance regulation Microbiology QR1-502 Ryuichi Nakano Akiyo Nakano Hisakazu Yano Ryoichi Okamoto Role of AmpR in the High Expression of the Plasmid-Encoded AmpC β-Lactamase CFE-1
description	ABSTRACT CFE-1 is a unique plasmid-encoded AmpC β-lactamase with the regulator gene ampR. It imparts high resistance to most cephalosporins with constitutive high-level β-lactamase activity. Here, the β-lactamase activities and expression levels of ampC with or without ampR were investigated. Results suggested that the resistance of CFE-1 to cephalosporins is caused by a substitution in AmpR, in which the Asp at position 135 is modified to Ala to allow the constitutive high-level expression (derepression) of ampC.
format	article
author	Ryuichi Nakano Akiyo Nakano Hisakazu Yano Ryoichi Okamoto
author_facet	Ryuichi Nakano Akiyo Nakano Hisakazu Yano Ryoichi Okamoto
author_sort	Ryuichi Nakano
title	Role of AmpR in the High Expression of the Plasmid-Encoded AmpC β-Lactamase CFE-1
title_short	Role of AmpR in the High Expression of the Plasmid-Encoded AmpC β-Lactamase CFE-1
title_full	Role of AmpR in the High Expression of the Plasmid-Encoded AmpC β-Lactamase CFE-1
title_fullStr	Role of AmpR in the High Expression of the Plasmid-Encoded AmpC β-Lactamase CFE-1
title_full_unstemmed	Role of AmpR in the High Expression of the Plasmid-Encoded AmpC β-Lactamase CFE-1
title_sort	role of ampr in the high expression of the plasmid-encoded ampc β-lactamase cfe-1
publisher	American Society for Microbiology
publishDate	2017
url	https://doaj.org/article/97d56aac9fb84060bedb3085d6093c89
work_keys_str_mv	AT ryuichinakano roleofamprinthehighexpressionoftheplasmidencodedampcblactamasecfe1 AT akiyonakano roleofamprinthehighexpressionoftheplasmidencodedampcblactamasecfe1 AT hisakazuyano roleofamprinthehighexpressionoftheplasmidencodedampcblactamasecfe1 AT ryoichiokamoto roleofamprinthehighexpressionoftheplasmidencodedampcblactamasecfe1
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Role of AmpR in the High Expression of the Plasmid-Encoded AmpC β-Lactamase CFE-1

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