Selective Laser Efficiency of Green-Synthesized Silver Nanoparticles by Aloe arborescens and Its Wound Healing Activities in Normal Wounded and Diabetic Wounded Fibroblast Cells: In vitro Studies

Sathish Sundar Dhilip Kumar, Nicolette Nadene Houreld, Heidi Abrahamse Laser Research Centre, University of Johannesburg, Johannesburg, South AfricaCorrespondence: Sathish Sundar Dhilip KumarLaser Research Centre, Faculty of Health Sciences, University of Johannesburg, PO Box 17011, Doornfontein, Jo...

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Autores principales:	Dhilip Kumar SS, Houreld NN, Abrahamse H
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	green synthesis silver nanoparticles photobiomodulation laser wound healing cell migration Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/97d5c5f573db4c48ab36bd98276c8afb
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spelling	oai:doaj.org-article:97d5c5f573db4c48ab36bd98276c8afb2021-12-02T11:13:19ZSelective Laser Efficiency of Green-Synthesized Silver Nanoparticles by Aloe arborescens and Its Wound Healing Activities in Normal Wounded and Diabetic Wounded Fibroblast Cells: In vitro Studies1178-2013https://doaj.org/article/97d5c5f573db4c48ab36bd98276c8afb2020-09-01T00:00:00Zhttps://www.dovepress.com/selective-laser-efficiency-of-green-synthesized-silver-nanoparticles-b-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Sathish Sundar Dhilip Kumar, Nicolette Nadene Houreld, Heidi Abrahamse Laser Research Centre, University of Johannesburg, Johannesburg, South AfricaCorrespondence: Sathish Sundar Dhilip KumarLaser Research Centre, Faculty of Health Sciences, University of Johannesburg, PO Box 17011, Doornfontein, Johannesburg 2028, South AfricaFax +27 11 559 6884Email sathishd@uj.ac.zaIntroduction: Silver nanoparticles (AgNPs) have been extensively used in wound healing applications owing to their valuable physicochemical and biological properties. The main objective of this study was to evaluate the combined effects of green-synthesized silver nanoparticles (G-AgNPs) and photobiomodulation (PBM; laser irradiation at 830 nm with 5 J/cm2) in normal wounded and diabetic wounded fibroblast cells (WS1).Methods: The combined effect of G-AgNPs and PBM was studied by various in vitro wound healing studies including cell morphology, cell migration rate and percentage wound closure, cell viability, cell proliferation, and filamentous (F)-actin and nuclear morphology staining.Results: Cell viability results revealed good cellular compatibility of G-AgNPs to WS1 cells. The combined therapy of G-AgNPs and PBM demonstrated promising results to achieve progressive migration and wound closure in both normal wounded and diabetic wounded cell models. G-AgNPs alone and in combination with PBM had no negative effect on cell viability and proliferation, and there was an increase in cell migration.Conclusion: Overall, these findings demonstrate that the combined treatment of G-AgNPs and PBM does not display any adverse effects on wound healing processes in both normal wounded and diabetic wounded cell models.Keywords: green synthesis, silver nanoparticles, photobiomodulation, laser, wound healing, cell migrationDhilip Kumar SSHoureld NNAbrahamse HDove Medical Pressarticlegreen synthesissilver nanoparticlesphotobiomodulationlaserwound healingcell migrationMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 15, Pp 6855-6870 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	green synthesis silver nanoparticles photobiomodulation laser wound healing cell migration Medicine (General) R5-920
spellingShingle	green synthesis silver nanoparticles photobiomodulation laser wound healing cell migration Medicine (General) R5-920 Dhilip Kumar SS Houreld NN Abrahamse H Selective Laser Efficiency of Green-Synthesized Silver Nanoparticles by Aloe arborescens and Its Wound Healing Activities in Normal Wounded and Diabetic Wounded Fibroblast Cells: In vitro Studies
description	Sathish Sundar Dhilip Kumar, Nicolette Nadene Houreld, Heidi Abrahamse Laser Research Centre, University of Johannesburg, Johannesburg, South AfricaCorrespondence: Sathish Sundar Dhilip KumarLaser Research Centre, Faculty of Health Sciences, University of Johannesburg, PO Box 17011, Doornfontein, Johannesburg 2028, South AfricaFax +27 11 559 6884Email sathishd@uj.ac.zaIntroduction: Silver nanoparticles (AgNPs) have been extensively used in wound healing applications owing to their valuable physicochemical and biological properties. The main objective of this study was to evaluate the combined effects of green-synthesized silver nanoparticles (G-AgNPs) and photobiomodulation (PBM; laser irradiation at 830 nm with 5 J/cm2) in normal wounded and diabetic wounded fibroblast cells (WS1).Methods: The combined effect of G-AgNPs and PBM was studied by various in vitro wound healing studies including cell morphology, cell migration rate and percentage wound closure, cell viability, cell proliferation, and filamentous (F)-actin and nuclear morphology staining.Results: Cell viability results revealed good cellular compatibility of G-AgNPs to WS1 cells. The combined therapy of G-AgNPs and PBM demonstrated promising results to achieve progressive migration and wound closure in both normal wounded and diabetic wounded cell models. G-AgNPs alone and in combination with PBM had no negative effect on cell viability and proliferation, and there was an increase in cell migration.Conclusion: Overall, these findings demonstrate that the combined treatment of G-AgNPs and PBM does not display any adverse effects on wound healing processes in both normal wounded and diabetic wounded cell models.Keywords: green synthesis, silver nanoparticles, photobiomodulation, laser, wound healing, cell migration
format	article
author	Dhilip Kumar SS Houreld NN Abrahamse H
author_facet	Dhilip Kumar SS Houreld NN Abrahamse H
author_sort	Dhilip Kumar SS
title	Selective Laser Efficiency of Green-Synthesized Silver Nanoparticles by Aloe arborescens and Its Wound Healing Activities in Normal Wounded and Diabetic Wounded Fibroblast Cells: In vitro Studies
title_short	Selective Laser Efficiency of Green-Synthesized Silver Nanoparticles by Aloe arborescens and Its Wound Healing Activities in Normal Wounded and Diabetic Wounded Fibroblast Cells: In vitro Studies
title_full	Selective Laser Efficiency of Green-Synthesized Silver Nanoparticles by Aloe arborescens and Its Wound Healing Activities in Normal Wounded and Diabetic Wounded Fibroblast Cells: In vitro Studies
title_fullStr	Selective Laser Efficiency of Green-Synthesized Silver Nanoparticles by Aloe arborescens and Its Wound Healing Activities in Normal Wounded and Diabetic Wounded Fibroblast Cells: In vitro Studies
title_full_unstemmed	Selective Laser Efficiency of Green-Synthesized Silver Nanoparticles by Aloe arborescens and Its Wound Healing Activities in Normal Wounded and Diabetic Wounded Fibroblast Cells: In vitro Studies
title_sort	selective laser efficiency of green-synthesized silver nanoparticles by aloe arborescens and its wound healing activities in normal wounded and diabetic wounded fibroblast cells: in vitro studies
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/97d5c5f573db4c48ab36bd98276c8afb
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Selective Laser Efficiency of Green-Synthesized Silver Nanoparticles by Aloe arborescens and Its Wound Healing Activities in Normal Wounded and Diabetic Wounded Fibroblast Cells: In vitro Studies

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