Identification of miRNAs and their target genes in genic male sterility lines in Brassica napus by small RNA sequencing

Abstract Background Brassica napus is the third leading source of edible oil in the world. Genic male sterility (GMS) lines provide crucial material for harnessing heterosis for rapeseed. GMS lines have been used successfully for rapeseed hybrid production in China. MicroRNAs (miRNAs) play crucial r...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Jianxia Jiang, Pengfei Xu, Yajie Li, Yanli Li, Xirong Zhou, Meiyan Jiang, Junying Zhang, Jifeng Zhu, Weirong Wang, Liyong Yang
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Brassica napus miRNAs Genic male sterility Pollen development miR159 Silique development Botany QK1-989
Acceso en línea:	https://doaj.org/article/97d9bfe992044f2eb42623497d58586a
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:97d9bfe992044f2eb42623497d58586a
record_format	dspace
spelling	oai:doaj.org-article:97d9bfe992044f2eb42623497d58586a2021-11-14T12:09:18ZIdentification of miRNAs and their target genes in genic male sterility lines in Brassica napus by small RNA sequencing10.1186/s12870-021-03306-w1471-2229https://doaj.org/article/97d9bfe992044f2eb42623497d58586a2021-11-01T00:00:00Zhttps://doi.org/10.1186/s12870-021-03306-whttps://doaj.org/toc/1471-2229Abstract Background Brassica napus is the third leading source of edible oil in the world. Genic male sterility (GMS) lines provide crucial material for harnessing heterosis for rapeseed. GMS lines have been used successfully for rapeseed hybrid production in China. MicroRNAs (miRNAs) play crucial regulatory roles in various plant growth, development, and stress response processes. However, reports on miRNAs that regulate the pollen development of GMS lines in B. napus are few. Results In this study, 12 small RNA and transcriptome libraries were constructed and sequenced for the flower buds from the fertile and sterile lines of two recessive GMS (RGMS) lines, namely, “6251AB” and “6284AB”. At the same time, 12 small RNA and transcriptome libraries were also constructed and sequenced for the flower buds from the fertile and sterile lines of two dominant GMS (DGMS) lines, namely, “4001AB” and “4006AB”. Based on the results, 46 known miRNAs, 27 novel miRNAs on the other arm of known pre-miRNAs, and 44 new conserved miRNAs were identified. Thirty-five pairs of novel miRNA-3p/miRNA-5p were found. Among all the identified miRNAs, fifteen differentially expressed miRNAs with over 1.5-fold change between flower buds of sterile and fertile lines were identified, including six differentially expressed miRNAs between “4001A” and “4001B”, two differentially expressed miRNAs between “4006A” and “4006B”, four differentially expressed miRNAs between “6251A” and “6251B”, and ten differentially expressed miRNAs between “6284A” and “6284B”. The correlation analysis of small RNA and transcriptome sequencing was conducted. And 257 candidate target genes were predicted for the 15 differentially expressed miRNAs. The results of 5′ modified RACE indicated that BnaA09g48720D, BnaA09g11120D, and BnaCnng51960D were cleaved by bna-miR398a-3p, bna-miR158-3p and bna-miR159a, respectively. Among the differentially expressed miRNAs, miR159 was chosen to analyze its function. Overexpression of bna-miR159 in Arabidopsis resulted in decreased seed setting rate, and shortened siliques, illustrating that miR159 may regulate the fertility and silique development in rapeseed. Conclusions Our findings provide an overview of miRNAs that are potentially involved in GMS and pollen development. New information on miRNAs and their related target genes are provided to exploit the GMS mechanism and reveal the miRNA networks in B. napus.Jianxia JiangPengfei XuYajie LiYanli LiXirong ZhouMeiyan JiangJunying ZhangJifeng ZhuWeirong WangLiyong YangBMCarticleBrassica napusmiRNAsGenic male sterilityPollen developmentmiR159Silique developmentBotanyQK1-989ENBMC Plant Biology, Vol 21, Iss 1, Pp 1-18 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Brassica napus miRNAs Genic male sterility Pollen development miR159 Silique development Botany QK1-989
spellingShingle	Brassica napus miRNAs Genic male sterility Pollen development miR159 Silique development Botany QK1-989 Jianxia Jiang Pengfei Xu Yajie Li Yanli Li Xirong Zhou Meiyan Jiang Junying Zhang Jifeng Zhu Weirong Wang Liyong Yang Identification of miRNAs and their target genes in genic male sterility lines in Brassica napus by small RNA sequencing
description	Abstract Background Brassica napus is the third leading source of edible oil in the world. Genic male sterility (GMS) lines provide crucial material for harnessing heterosis for rapeseed. GMS lines have been used successfully for rapeseed hybrid production in China. MicroRNAs (miRNAs) play crucial regulatory roles in various plant growth, development, and stress response processes. However, reports on miRNAs that regulate the pollen development of GMS lines in B. napus are few. Results In this study, 12 small RNA and transcriptome libraries were constructed and sequenced for the flower buds from the fertile and sterile lines of two recessive GMS (RGMS) lines, namely, “6251AB” and “6284AB”. At the same time, 12 small RNA and transcriptome libraries were also constructed and sequenced for the flower buds from the fertile and sterile lines of two dominant GMS (DGMS) lines, namely, “4001AB” and “4006AB”. Based on the results, 46 known miRNAs, 27 novel miRNAs on the other arm of known pre-miRNAs, and 44 new conserved miRNAs were identified. Thirty-five pairs of novel miRNA-3p/miRNA-5p were found. Among all the identified miRNAs, fifteen differentially expressed miRNAs with over 1.5-fold change between flower buds of sterile and fertile lines were identified, including six differentially expressed miRNAs between “4001A” and “4001B”, two differentially expressed miRNAs between “4006A” and “4006B”, four differentially expressed miRNAs between “6251A” and “6251B”, and ten differentially expressed miRNAs between “6284A” and “6284B”. The correlation analysis of small RNA and transcriptome sequencing was conducted. And 257 candidate target genes were predicted for the 15 differentially expressed miRNAs. The results of 5′ modified RACE indicated that BnaA09g48720D, BnaA09g11120D, and BnaCnng51960D were cleaved by bna-miR398a-3p, bna-miR158-3p and bna-miR159a, respectively. Among the differentially expressed miRNAs, miR159 was chosen to analyze its function. Overexpression of bna-miR159 in Arabidopsis resulted in decreased seed setting rate, and shortened siliques, illustrating that miR159 may regulate the fertility and silique development in rapeseed. Conclusions Our findings provide an overview of miRNAs that are potentially involved in GMS and pollen development. New information on miRNAs and their related target genes are provided to exploit the GMS mechanism and reveal the miRNA networks in B. napus.
format	article
author	Jianxia Jiang Pengfei Xu Yajie Li Yanli Li Xirong Zhou Meiyan Jiang Junying Zhang Jifeng Zhu Weirong Wang Liyong Yang
author_facet	Jianxia Jiang Pengfei Xu Yajie Li Yanli Li Xirong Zhou Meiyan Jiang Junying Zhang Jifeng Zhu Weirong Wang Liyong Yang
author_sort	Jianxia Jiang
title	Identification of miRNAs and their target genes in genic male sterility lines in Brassica napus by small RNA sequencing
title_short	Identification of miRNAs and their target genes in genic male sterility lines in Brassica napus by small RNA sequencing
title_full	Identification of miRNAs and their target genes in genic male sterility lines in Brassica napus by small RNA sequencing
title_fullStr	Identification of miRNAs and their target genes in genic male sterility lines in Brassica napus by small RNA sequencing
title_full_unstemmed	Identification of miRNAs and their target genes in genic male sterility lines in Brassica napus by small RNA sequencing
title_sort	identification of mirnas and their target genes in genic male sterility lines in brassica napus by small rna sequencing
publisher	BMC
publishDate	2021
url	https://doaj.org/article/97d9bfe992044f2eb42623497d58586a
work_keys_str_mv	AT jianxiajiang identificationofmirnasandtheirtargetgenesingenicmalesterilitylinesinbrassicanapusbysmallrnasequencing AT pengfeixu identificationofmirnasandtheirtargetgenesingenicmalesterilitylinesinbrassicanapusbysmallrnasequencing AT yajieli identificationofmirnasandtheirtargetgenesingenicmalesterilitylinesinbrassicanapusbysmallrnasequencing AT yanlili identificationofmirnasandtheirtargetgenesingenicmalesterilitylinesinbrassicanapusbysmallrnasequencing AT xirongzhou identificationofmirnasandtheirtargetgenesingenicmalesterilitylinesinbrassicanapusbysmallrnasequencing AT meiyanjiang identificationofmirnasandtheirtargetgenesingenicmalesterilitylinesinbrassicanapusbysmallrnasequencing AT junyingzhang identificationofmirnasandtheirtargetgenesingenicmalesterilitylinesinbrassicanapusbysmallrnasequencing AT jifengzhu identificationofmirnasandtheirtargetgenesingenicmalesterilitylinesinbrassicanapusbysmallrnasequencing AT weirongwang identificationofmirnasandtheirtargetgenesingenicmalesterilitylinesinbrassicanapusbysmallrnasequencing AT liyongyang identificationofmirnasandtheirtargetgenesingenicmalesterilitylinesinbrassicanapusbysmallrnasequencing
_version_	1718429418804740096

Identification of miRNAs and their target genes in genic male sterility lines in Brassica napus by small RNA sequencing

Ejemplares similares