Detection of meningococcus, pneumococcus, Haemophilus influenzae, and group A streptococcus DNA in pediatric adenoid bioptats

Meningococcal, pneumococcal, streptococcal A and Haemophilus influenzae infections are manifested in different clinical forms, ranging from bacterial carriage to generalized life-threatening conditions. However, a connection between bacterial carriage and disease development has not been fully explo...

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Autores principales:	S. Yu. Kombarova, A. M. Bichucher, Y. L. Soldatsky, R. Yu. Yunusova, T. A. Skirda, I. G. Martynenko, L. I. Golovina, S. R. Edgem, T. V. Severin, V. G. Melnikov
Formato:	article
Lenguaje:	RU
Publicado:	Sankt-Peterburg : NIIÈM imeni Pastera 2020
Materias:	neisseria meningitidis streptococcus pneumoniae streptococcus pyogenes haemophilus influenzae bacterial carriage adenoid bioptats pcr Infectious and parasitic diseases RC109-216
Acceso en línea:	https://doaj.org/article/97ddf4255f0a429b8efaffbdacd77725
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spelling	oai:doaj.org-article:97ddf4255f0a429b8efaffbdacd777252021-11-22T07:09:53ZDetection of meningococcus, pneumococcus, Haemophilus influenzae, and group A streptococcus DNA in pediatric adenoid bioptats2220-76192313-739810.15789/2220-7619-DOM-1163https://doaj.org/article/97ddf4255f0a429b8efaffbdacd777252020-04-01T00:00:00Zhttps://www.iimmun.ru/iimm/article/view/1163https://doaj.org/toc/2220-7619https://doaj.org/toc/2313-7398Meningococcal, pneumococcal, streptococcal A and Haemophilus influenzae infections are manifested in different clinical forms, ranging from bacterial carriage to generalized life-threatening conditions. However, a connection between bacterial carriage and disease development has not been fully explored. A PCR assay was performed with adenoid biopsy samples collected from 112 children after planned adenotomy to detect Neisseria meningitidis, Streptococcus pneumoniae, Streptococcus pyogenes, H. influenzae carriage. A DNA specific to at least one of the four studied microbial species was found in 104 samples (92.86%) so that: meningococcal DNA was detected in one sample (0.9%), pneumococcal — in 98 (87.5%), H. influenzae — in 19 (16.96%), and streptococcal A — in 42 (37.5%) samples. However, none of these species was found in 8 children (7.14%). A sole S. pneumoniae was detected in 54 samples (48.2%), whereas S. pyogenes — in 5 samples (4.5%). Moreover, two bacterial species were simultaneously as follows: N. meningitidis and S. pneumoniae — in 1 sample (0.9%), S. pneumoniae and H. influenzae — in 7 samples (6.3%); H. influenzae and S. pyogenes — in 1 sample (0.9%); S. pneumoniae and S. pyogenes — in 25 samples (22.3%). A triple combination consisting of S. pneumoniae, H. influenzae and S. pyogenes bacteria were detected together in 11 patients (9.8%). Meningococcal serogrouping revealed no connection with any of the 6 most common global serogroups responsible for epidemic incidence rise (A, B, C, W-135, X, Y). A clear tendency for prevalence of S. pyogenes DNA in adenoid pediatric biopsies in children diagnosed with “Adenoids and tonsils hypertrophy” vs. “Adenoids hypertrophy” was observed. It is noteworthy, a high relative prevalence of pneumococcal carriage (87.5%), found by us was of special importance. Pediatric carriers serving as a reservoir for virulent pneumococcal species pose a threat both for themselves and surrounding people. Thus, PCR-based data of adenoid biopsies may be a promising approach for future studies, as a potential to identify live viable but nonculturable bacteria in clinical specimens will contribute to a more accurate assessment of carriage rate of meningococci, pneumococci, H. influenzae and group A streptococci.S. Yu. KombarovaA. M. BichucherY. L. SoldatskyR. Yu. YunusovaT. A. SkirdaI. G. MartynenkoL. I. GolovinaS. R. EdgemT. V. SeverinV. G. MelnikovSankt-Peterburg : NIIÈM imeni Pasteraarticleneisseria meningitidisstreptococcus pneumoniaestreptococcus pyogeneshaemophilus influenzaebacterial carriageadenoid bioptatspcrInfectious and parasitic diseasesRC109-216RUInfekciâ i Immunitet, Vol 10, Iss 1, Pp 111-120 (2020)
institution	DOAJ
collection	DOAJ
language	RU
topic	neisseria meningitidis streptococcus pneumoniae streptococcus pyogenes haemophilus influenzae bacterial carriage adenoid bioptats pcr Infectious and parasitic diseases RC109-216
spellingShingle	neisseria meningitidis streptococcus pneumoniae streptococcus pyogenes haemophilus influenzae bacterial carriage adenoid bioptats pcr Infectious and parasitic diseases RC109-216 S. Yu. Kombarova A. M. Bichucher Y. L. Soldatsky R. Yu. Yunusova T. A. Skirda I. G. Martynenko L. I. Golovina S. R. Edgem T. V. Severin V. G. Melnikov Detection of meningococcus, pneumococcus, Haemophilus influenzae, and group A streptococcus DNA in pediatric adenoid bioptats
description	Meningococcal, pneumococcal, streptococcal A and Haemophilus influenzae infections are manifested in different clinical forms, ranging from bacterial carriage to generalized life-threatening conditions. However, a connection between bacterial carriage and disease development has not been fully explored. A PCR assay was performed with adenoid biopsy samples collected from 112 children after planned adenotomy to detect Neisseria meningitidis, Streptococcus pneumoniae, Streptococcus pyogenes, H. influenzae carriage. A DNA specific to at least one of the four studied microbial species was found in 104 samples (92.86%) so that: meningococcal DNA was detected in one sample (0.9%), pneumococcal — in 98 (87.5%), H. influenzae — in 19 (16.96%), and streptococcal A — in 42 (37.5%) samples. However, none of these species was found in 8 children (7.14%). A sole S. pneumoniae was detected in 54 samples (48.2%), whereas S. pyogenes — in 5 samples (4.5%). Moreover, two bacterial species were simultaneously as follows: N. meningitidis and S. pneumoniae — in 1 sample (0.9%), S. pneumoniae and H. influenzae — in 7 samples (6.3%); H. influenzae and S. pyogenes — in 1 sample (0.9%); S. pneumoniae and S. pyogenes — in 25 samples (22.3%). A triple combination consisting of S. pneumoniae, H. influenzae and S. pyogenes bacteria were detected together in 11 patients (9.8%). Meningococcal serogrouping revealed no connection with any of the 6 most common global serogroups responsible for epidemic incidence rise (A, B, C, W-135, X, Y). A clear tendency for prevalence of S. pyogenes DNA in adenoid pediatric biopsies in children diagnosed with “Adenoids and tonsils hypertrophy” vs. “Adenoids hypertrophy” was observed. It is noteworthy, a high relative prevalence of pneumococcal carriage (87.5%), found by us was of special importance. Pediatric carriers serving as a reservoir for virulent pneumococcal species pose a threat both for themselves and surrounding people. Thus, PCR-based data of adenoid biopsies may be a promising approach for future studies, as a potential to identify live viable but nonculturable bacteria in clinical specimens will contribute to a more accurate assessment of carriage rate of meningococci, pneumococci, H. influenzae and group A streptococci.
format	article
author	S. Yu. Kombarova A. M. Bichucher Y. L. Soldatsky R. Yu. Yunusova T. A. Skirda I. G. Martynenko L. I. Golovina S. R. Edgem T. V. Severin V. G. Melnikov
author_facet	S. Yu. Kombarova A. M. Bichucher Y. L. Soldatsky R. Yu. Yunusova T. A. Skirda I. G. Martynenko L. I. Golovina S. R. Edgem T. V. Severin V. G. Melnikov
author_sort	S. Yu. Kombarova
title	Detection of meningococcus, pneumococcus, Haemophilus influenzae, and group A streptococcus DNA in pediatric adenoid bioptats
title_short	Detection of meningococcus, pneumococcus, Haemophilus influenzae, and group A streptococcus DNA in pediatric adenoid bioptats
title_full	Detection of meningococcus, pneumococcus, Haemophilus influenzae, and group A streptococcus DNA in pediatric adenoid bioptats
title_fullStr	Detection of meningococcus, pneumococcus, Haemophilus influenzae, and group A streptococcus DNA in pediatric adenoid bioptats
title_full_unstemmed	Detection of meningococcus, pneumococcus, Haemophilus influenzae, and group A streptococcus DNA in pediatric adenoid bioptats
title_sort	detection of meningococcus, pneumococcus, haemophilus influenzae, and group a streptococcus dna in pediatric adenoid bioptats
publisher	Sankt-Peterburg : NIIÈM imeni Pastera
publishDate	2020
url	https://doaj.org/article/97ddf4255f0a429b8efaffbdacd77725
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Detection of meningococcus, pneumococcus, Haemophilus influenzae, and group A streptococcus DNA in pediatric adenoid bioptats

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