QTL for seed shattering and threshability in intermediate wheatgrass align closely with well‐studied orthologs from wheat, barley, and rice

Abstract Perennial grain crops have the potential to improve agricultural sustainability but few existing species produce sufficient grain yield to be economically viable. The outcrossing, allohexaploid, and perennial forage species intermediate wheatgrass (IWG) [Thinopyrum intermedium (Host) Barkwo...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Kayla R. Altendorf, Lee R. DeHaan, Steve R. Larson, James A. Anderson
Formato:	article
Lenguaje:	EN
Publicado:	Wiley 2021
Materias:	Plant culture SB1-1110 Genetics QH426-470
Acceso en línea:	https://doaj.org/article/97fa8944545746688a46e75e01bf66ab
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:97fa8944545746688a46e75e01bf66ab
record_format	dspace
spelling	oai:doaj.org-article:97fa8944545746688a46e75e01bf66ab2021-12-05T07:50:12ZQTL for seed shattering and threshability in intermediate wheatgrass align closely with well‐studied orthologs from wheat, barley, and rice1940-337210.1002/tpg2.20145https://doaj.org/article/97fa8944545746688a46e75e01bf66ab2021-11-01T00:00:00Zhttps://doi.org/10.1002/tpg2.20145https://doaj.org/toc/1940-3372Abstract Perennial grain crops have the potential to improve agricultural sustainability but few existing species produce sufficient grain yield to be economically viable. The outcrossing, allohexaploid, and perennial forage species intermediate wheatgrass (IWG) [Thinopyrum intermedium (Host) Barkworth & D. R. Dewey] has shown promise in undergoing direct domestication as a perennial grain crop using phenotypic and genomic selection. However, decades of selection will be required to achieve yields on par with annual small‐grain crops. Marker‐aided selection could accelerate progress if important genomic regions associated with domestication were identified. Here we use the IWG nested association mapping (NAM) population, with 1,168 F1 progeny across 10 families to dissect the genetic control of brittle rachis, floret shattering, and threshability. We used a genome‐wide association study (GWAS) with 8,003 single nucleotide polymorphism (SNP) markers and linkage mapping—both within‐family and combined across families—with a robust phenotypic dataset collected from four unique year‐by‐location combinations. A total of 29 quantitative trait loci (QTL) using GWAS and 20 using the combined linkage analysis were detected, and most large‐effect QTL were in common across the two analysis methods. We reveal that the genetic control of these traits in IWG is complex, with significant QTL across multiple chromosomes, sometimes within and across homoeologous groups and effects that vary depending on the family. In some cases, these QTL align within 216 bp to 31 Mbp of BLAST hits for known domestication genes in related species and may serve as precise targets of selection and directions for further study to advance the domestication of IWG.Kayla R. AltendorfLee R. DeHaanSteve R. LarsonJames A. AndersonWileyarticlePlant cultureSB1-1110GeneticsQH426-470ENThe Plant Genome, Vol 14, Iss 3, Pp n/a-n/a (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Plant culture SB1-1110 Genetics QH426-470
spellingShingle	Plant culture SB1-1110 Genetics QH426-470 Kayla R. Altendorf Lee R. DeHaan Steve R. Larson James A. Anderson QTL for seed shattering and threshability in intermediate wheatgrass align closely with well‐studied orthologs from wheat, barley, and rice
description	Abstract Perennial grain crops have the potential to improve agricultural sustainability but few existing species produce sufficient grain yield to be economically viable. The outcrossing, allohexaploid, and perennial forage species intermediate wheatgrass (IWG) [Thinopyrum intermedium (Host) Barkworth & D. R. Dewey] has shown promise in undergoing direct domestication as a perennial grain crop using phenotypic and genomic selection. However, decades of selection will be required to achieve yields on par with annual small‐grain crops. Marker‐aided selection could accelerate progress if important genomic regions associated with domestication were identified. Here we use the IWG nested association mapping (NAM) population, with 1,168 F1 progeny across 10 families to dissect the genetic control of brittle rachis, floret shattering, and threshability. We used a genome‐wide association study (GWAS) with 8,003 single nucleotide polymorphism (SNP) markers and linkage mapping—both within‐family and combined across families—with a robust phenotypic dataset collected from four unique year‐by‐location combinations. A total of 29 quantitative trait loci (QTL) using GWAS and 20 using the combined linkage analysis were detected, and most large‐effect QTL were in common across the two analysis methods. We reveal that the genetic control of these traits in IWG is complex, with significant QTL across multiple chromosomes, sometimes within and across homoeologous groups and effects that vary depending on the family. In some cases, these QTL align within 216 bp to 31 Mbp of BLAST hits for known domestication genes in related species and may serve as precise targets of selection and directions for further study to advance the domestication of IWG.
format	article
author	Kayla R. Altendorf Lee R. DeHaan Steve R. Larson James A. Anderson
author_facet	Kayla R. Altendorf Lee R. DeHaan Steve R. Larson James A. Anderson
author_sort	Kayla R. Altendorf
title	QTL for seed shattering and threshability in intermediate wheatgrass align closely with well‐studied orthologs from wheat, barley, and rice
title_short	QTL for seed shattering and threshability in intermediate wheatgrass align closely with well‐studied orthologs from wheat, barley, and rice
title_full	QTL for seed shattering and threshability in intermediate wheatgrass align closely with well‐studied orthologs from wheat, barley, and rice
title_fullStr	QTL for seed shattering and threshability in intermediate wheatgrass align closely with well‐studied orthologs from wheat, barley, and rice
title_full_unstemmed	QTL for seed shattering and threshability in intermediate wheatgrass align closely with well‐studied orthologs from wheat, barley, and rice
title_sort	qtl for seed shattering and threshability in intermediate wheatgrass align closely with well‐studied orthologs from wheat, barley, and rice
publisher	Wiley
publishDate	2021
url	https://doaj.org/article/97fa8944545746688a46e75e01bf66ab
work_keys_str_mv	AT kaylaraltendorf qtlforseedshatteringandthreshabilityinintermediatewheatgrassaligncloselywithwellstudiedorthologsfromwheatbarleyandrice AT leerdehaan qtlforseedshatteringandthreshabilityinintermediatewheatgrassaligncloselywithwellstudiedorthologsfromwheatbarleyandrice AT steverlarson qtlforseedshatteringandthreshabilityinintermediatewheatgrassaligncloselywithwellstudiedorthologsfromwheatbarleyandrice AT jamesaanderson qtlforseedshatteringandthreshabilityinintermediatewheatgrassaligncloselywithwellstudiedorthologsfromwheatbarleyandrice
_version_	1718372570527432704

QTL for seed shattering and threshability in intermediate wheatgrass align closely with well‐studied orthologs from wheat, barley, and rice

Ejemplares similares