Extra virgin olive oil improved body weight and insulin sensitivity in high fat diet-induced obese LDLr−/−.Leiden mice without attenuation of steatohepatitis

Abstract Dietary fatty acids play a role in the pathogenesis of obesity-associated non-alcoholic fatty liver disease (NAFLD), which is associated with insulin resistance (IR). Fatty acid composition is critical for IR and subsequent NAFLD development. Extra-virgin olive oil (EVOO) is the main source...

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Autores principales:	Leticia Álvarez-Amor, Amparo Luque Sierra, Antonio Cárdenas, Lucía López-Bermudo, Javier López-Beas, Eloísa Andújar, Mónica Pérez-Alegre, Rocío Gallego-Durán, Lourdes M. Varela, Alejandro Martin-Montalvo, Genoveva Berná, Anabel Rojas, Mª José Robles-Frías, Abdelkrim Hmadcha, Manuel Romero-Gómez, Robert Kleemann, Franz Martín
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/98018cb0ec61418692f64df0f9c6509c
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spelling	oai:doaj.org-article:98018cb0ec61418692f64df0f9c6509c2021-12-02T18:02:48ZExtra virgin olive oil improved body weight and insulin sensitivity in high fat diet-induced obese LDLr−/−.Leiden mice without attenuation of steatohepatitis10.1038/s41598-021-87761-32045-2322https://doaj.org/article/98018cb0ec61418692f64df0f9c6509c2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87761-3https://doaj.org/toc/2045-2322Abstract Dietary fatty acids play a role in the pathogenesis of obesity-associated non-alcoholic fatty liver disease (NAFLD), which is associated with insulin resistance (IR). Fatty acid composition is critical for IR and subsequent NAFLD development. Extra-virgin olive oil (EVOO) is the main source of monounsaturated fatty acids (MUFA) in Mediterranean diets. This study examined whether EVOO-containing high fat diets may prevent diet-induced NAFLD using Ldlr−/−. Leiden mice. In female Ldlr−/−.Leiden mice, the effects of the following high fat diets (HFDs) were examined: a lard-based HFD (HFD-L); an EVOO-based HFD (HFD-EVOO); a phenolic compounds-rich EVOO HFD (HFD-OL). We studied changes in body weight (BW), lipid profile, transaminases, glucose homeostasis, liver pathology and transcriptome. Both EVOO diets reduced body weight (BW) and improved insulin sensitivity. The EVOOs did not improve transaminase values and increased LDL-cholesterol and liver collagen content. EVOOs and HFD-L groups had comparable liver steatosis. The profibrotic effects were substantiated by an up-regulation of gene transcripts related to glutathione metabolism, chemokine signaling and NF-kappa-B activation and down-regulation of genes relevant for fatty acid metabolism. Collectivelly, EVOO intake improved weight gain and insulin sensitivity but not liver inflammation and fibrosis, which was supported by changes in hepatic genes expression.Leticia Álvarez-AmorAmparo Luque SierraAntonio CárdenasLucía López-BermudoJavier López-BeasEloísa AndújarMónica Pérez-AlegreRocío Gallego-DuránLourdes M. VarelaAlejandro Martin-MontalvoGenoveva BernáAnabel RojasMª José Robles-FríasAbdelkrim HmadchaManuel Romero-GómezRobert KleemannFranz MartínNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Leticia Álvarez-Amor Amparo Luque Sierra Antonio Cárdenas Lucía López-Bermudo Javier López-Beas Eloísa Andújar Mónica Pérez-Alegre Rocío Gallego-Durán Lourdes M. Varela Alejandro Martin-Montalvo Genoveva Berná Anabel Rojas Mª José Robles-Frías Abdelkrim Hmadcha Manuel Romero-Gómez Robert Kleemann Franz Martín Extra virgin olive oil improved body weight and insulin sensitivity in high fat diet-induced obese LDLr−/−.Leiden mice without attenuation of steatohepatitis
description	Abstract Dietary fatty acids play a role in the pathogenesis of obesity-associated non-alcoholic fatty liver disease (NAFLD), which is associated with insulin resistance (IR). Fatty acid composition is critical for IR and subsequent NAFLD development. Extra-virgin olive oil (EVOO) is the main source of monounsaturated fatty acids (MUFA) in Mediterranean diets. This study examined whether EVOO-containing high fat diets may prevent diet-induced NAFLD using Ldlr−/−. Leiden mice. In female Ldlr−/−.Leiden mice, the effects of the following high fat diets (HFDs) were examined: a lard-based HFD (HFD-L); an EVOO-based HFD (HFD-EVOO); a phenolic compounds-rich EVOO HFD (HFD-OL). We studied changes in body weight (BW), lipid profile, transaminases, glucose homeostasis, liver pathology and transcriptome. Both EVOO diets reduced body weight (BW) and improved insulin sensitivity. The EVOOs did not improve transaminase values and increased LDL-cholesterol and liver collagen content. EVOOs and HFD-L groups had comparable liver steatosis. The profibrotic effects were substantiated by an up-regulation of gene transcripts related to glutathione metabolism, chemokine signaling and NF-kappa-B activation and down-regulation of genes relevant for fatty acid metabolism. Collectivelly, EVOO intake improved weight gain and insulin sensitivity but not liver inflammation and fibrosis, which was supported by changes in hepatic genes expression.
format	article
author	Leticia Álvarez-Amor Amparo Luque Sierra Antonio Cárdenas Lucía López-Bermudo Javier López-Beas Eloísa Andújar Mónica Pérez-Alegre Rocío Gallego-Durán Lourdes M. Varela Alejandro Martin-Montalvo Genoveva Berná Anabel Rojas Mª José Robles-Frías Abdelkrim Hmadcha Manuel Romero-Gómez Robert Kleemann Franz Martín
author_facet	Leticia Álvarez-Amor Amparo Luque Sierra Antonio Cárdenas Lucía López-Bermudo Javier López-Beas Eloísa Andújar Mónica Pérez-Alegre Rocío Gallego-Durán Lourdes M. Varela Alejandro Martin-Montalvo Genoveva Berná Anabel Rojas Mª José Robles-Frías Abdelkrim Hmadcha Manuel Romero-Gómez Robert Kleemann Franz Martín
author_sort	Leticia Álvarez-Amor
title	Extra virgin olive oil improved body weight and insulin sensitivity in high fat diet-induced obese LDLr−/−.Leiden mice without attenuation of steatohepatitis
title_short	Extra virgin olive oil improved body weight and insulin sensitivity in high fat diet-induced obese LDLr−/−.Leiden mice without attenuation of steatohepatitis
title_full	Extra virgin olive oil improved body weight and insulin sensitivity in high fat diet-induced obese LDLr−/−.Leiden mice without attenuation of steatohepatitis
title_fullStr	Extra virgin olive oil improved body weight and insulin sensitivity in high fat diet-induced obese LDLr−/−.Leiden mice without attenuation of steatohepatitis
title_full_unstemmed	Extra virgin olive oil improved body weight and insulin sensitivity in high fat diet-induced obese LDLr−/−.Leiden mice without attenuation of steatohepatitis
title_sort	extra virgin olive oil improved body weight and insulin sensitivity in high fat diet-induced obese ldlr−/−.leiden mice without attenuation of steatohepatitis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/98018cb0ec61418692f64df0f9c6509c
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Extra virgin olive oil improved body weight and insulin sensitivity in high fat diet-induced obese LDLr−/−.Leiden mice without attenuation of steatohepatitis

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