The effect of depression on fracture healing and osteoblast differentiation in rats

The effect of depression on fracture healing and osteoblast differentiation in rats

Chunzi Nie,1 Zhan Wang,2 Xufeng Liu1 1Department of Military Medical Psychology, Fourth Military Medical University, Xi’an 710032, People’s Republic of China; 2Department of Orthopaedics, Gansu Provincial Hospital, Lanzhou, Gansu 730050, People’s Republic of China Bac...

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Autores principales: Nie C, Wang Z, Liu X
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2018
Materias:
depression
fracture healing
osteoblast differentiation
autophagy
bone marrow mesenchymal stem cells
Neurosciences. Biological psychiatry. Neuropsychiatry
RC321-571
Neurology. Diseases of the nervous system
RC346-429
Acceso en línea:https://doaj.org/article/98080af75ba44b358dbb8a8ea5655840
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spelling oai:doaj.org-article:98080af75ba44b358dbb8a8ea56558402021-12-02T05:33:42ZThe effect of depression on fracture healing and osteoblast differentiation in rats1178-2021https://doaj.org/article/98080af75ba44b358dbb8a8ea56558402018-06-01T00:00:00Zhttps://www.dovepress.com/the-effect-of-depression-on-fracture-healing-and-osteoblast-differenti-peer-reviewed-article-NDThttps://doaj.org/toc/1178-2021Chunzi Nie,1 Zhan Wang,2 Xufeng Liu1 1Department of Military Medical Psychology, Fourth Military Medical University, Xi’an 710032, People’s Republic of China; 2Department of Orthopaedics, Gansu Provincial Hospital, Lanzhou, Gansu 730050, People’s Republic of China Background: Depressive disorder has been proven to be associated with disturbed bone metabolism. However, the effect of depression on fracture healing still lacks evidence. Materials and methods: A rat depressive model was first established by exposing the animals to chronic unpredictable stress, which was assessed using the sucrose preference test, forced swimming test, and open field test. Subsequently, the bone repairing ability was detected by micro-computed tomography and histological analysis of the femoral condyle defect rats with or without depression. To further investigate the potential mechanisms of depression on fracture healing, the osteogenic differentiation and autophagic level were compared between the bone marrow mesenchymal stem cells (BMSCs) derived from depressive and normal rats. Results: Our results showed that rats with depressive disorder significantly slowed the healing process at 4 and 8 weeks postinjury. Furthermore, the osteogenic potential and autophagy remarkably decreased in BMSCs from the depressive rats, suggesting an inherent relationship between autophagy and osteogenic differentiation. Finally, rapamycin, an autophagic agonist, significantly improved osteogenic differentiation of depressive BMSCs through autophagy activation. Conclusion: The present study indicated that depression had a negative effect on fracture healing with low osteoblast differentiation of BMSCs. Also, autophagy activation in BMSCs offers a novel therapeutic target for depressive patients with poor fracture healing. Keywords: depression, fracture healing, osteoblast differentiation, autophagy, bone marrow mesenchymal stem cellsNie CWang ZLiu XDove Medical Pressarticledepressionfracture healingosteoblast differentiationautophagybone marrow mesenchymal stem cellsNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571Neurology. Diseases of the nervous systemRC346-429ENNeuropsychiatric Disease and Treatment, Vol Volume 14, Pp 1705-1713 (2018)
institution DOAJ
collection DOAJ
language EN
topic depression
fracture healing
osteoblast differentiation
autophagy
bone marrow mesenchymal stem cells
Neurosciences. Biological psychiatry. Neuropsychiatry
RC321-571
Neurology. Diseases of the nervous system
RC346-429
spellingShingle depression
fracture healing
osteoblast differentiation
autophagy
bone marrow mesenchymal stem cells
Neurosciences. Biological psychiatry. Neuropsychiatry
RC321-571
Neurology. Diseases of the nervous system
RC346-429
Nie C
Wang Z
Liu X
The effect of depression on fracture healing and osteoblast differentiation in rats
description Chunzi Nie,1 Zhan Wang,2 Xufeng Liu1 1Department of Military Medical Psychology, Fourth Military Medical University, Xi’an 710032, People’s Republic of China; 2Department of Orthopaedics, Gansu Provincial Hospital, Lanzhou, Gansu 730050, People’s Republic of China Background: Depressive disorder has been proven to be associated with disturbed bone metabolism. However, the effect of depression on fracture healing still lacks evidence. Materials and methods: A rat depressive model was first established by exposing the animals to chronic unpredictable stress, which was assessed using the sucrose preference test, forced swimming test, and open field test. Subsequently, the bone repairing ability was detected by micro-computed tomography and histological analysis of the femoral condyle defect rats with or without depression. To further investigate the potential mechanisms of depression on fracture healing, the osteogenic differentiation and autophagic level were compared between the bone marrow mesenchymal stem cells (BMSCs) derived from depressive and normal rats. Results: Our results showed that rats with depressive disorder significantly slowed the healing process at 4 and 8 weeks postinjury. Furthermore, the osteogenic potential and autophagy remarkably decreased in BMSCs from the depressive rats, suggesting an inherent relationship between autophagy and osteogenic differentiation. Finally, rapamycin, an autophagic agonist, significantly improved osteogenic differentiation of depressive BMSCs through autophagy activation. Conclusion: The present study indicated that depression had a negative effect on fracture healing with low osteoblast differentiation of BMSCs. Also, autophagy activation in BMSCs offers a novel therapeutic target for depressive patients with poor fracture healing. Keywords: depression, fracture healing, osteoblast differentiation, autophagy, bone marrow mesenchymal stem cells
format article
author Nie C
Wang Z
Liu X
author_facet Nie C
Wang Z
Liu X
author_sort Nie C
title The effect of depression on fracture healing and osteoblast differentiation in rats
title_short The effect of depression on fracture healing and osteoblast differentiation in rats
title_full The effect of depression on fracture healing and osteoblast differentiation in rats
title_fullStr The effect of depression on fracture healing and osteoblast differentiation in rats
title_full_unstemmed The effect of depression on fracture healing and osteoblast differentiation in rats
title_sort effect of depression on fracture healing and osteoblast differentiation in rats
publisher Dove Medical Press
publishDate 2018
url https://doaj.org/article/98080af75ba44b358dbb8a8ea5655840
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