Swelling studies of camel and bovine corneal stroma

Turki Almubrad, Mohammad Faisal Jamal Khan, Saeed AkhtarCornea Research Chair, Department of Optometry, College of Applied Medical Sciences, King Saud University, Saudi ArabiaAbstract: In the present study we investigated the swelling characteristics of fresh camel and bovine cornea in sodium salt s...

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Autores principales:	Turki Almubrad, Mohammad Faisal Jamal Khan, Saeed Akhtar
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2010
Materias:	Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/980c5bac012449f5bd62182f6e89c0d8
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spelling	oai:doaj.org-article:980c5bac012449f5bd62182f6e89c0d82021-12-02T01:52:07ZSwelling studies of camel and bovine corneal stroma1177-54671177-5483https://doaj.org/article/980c5bac012449f5bd62182f6e89c0d82010-09-01T00:00:00Zhttp://www.dovepress.com/swelling-studies-of-camel-and-bovine-corneal-stroma-a5295https://doaj.org/toc/1177-5467https://doaj.org/toc/1177-5483Turki Almubrad, Mohammad Faisal Jamal Khan, Saeed AkhtarCornea Research Chair, Department of Optometry, College of Applied Medical Sciences, King Saud University, Saudi ArabiaAbstract: In the present study we investigated the swelling characteristics of fresh camel and bovine cornea in sodium salt solutions. Swelling studies were carried out at 20 minutes, 14 hours, and 46 hours on five fresh camel and 5 five fresh bovine corneas. During the 20-minute hydration of fresh corneal stroma was investigated using sodium chloride (NaCl), sodium bicarbonate (NaHCO3), sodium acetate (CH3COONa), sodium thiocyanate (NaSCN), and sodium floride (NaF) at 2-minute time intervals. During a 46-hour time period, the hydration study was carried out using NaCl (150, 300 mM) and NaF (150 mM) at random intervals. The 14-hour study was carried out to assess the rehydration of corneal stroma after 6 hours of drying. During the 20-minute swelling studies in the first 2 minutes the rate of hydration in both camel and bovine corneas was high but gradually reduced in the 2&ndash;20-minute period. The rates and levels of hydration of camel and bovine cornea were not significantly different from each other in all the strengths of solutions. During the 46-hour swelling studies, the initial rate of hydration (0&ndash;2 hours) of camel and bovine stroma, in all solutions was significantly higher (Z = 0.056) compared to hydration during later hours (2&ndash;46 hours). Camel stromal hydration (high) in 150 mM NaCl was significantly higher compared to bovine stromal hydration in the same solution during the 10&ndash;24, and 24&ndash;46-hour time periods. Rehydration in camel stroma was significantly lower than bovine in 150 mM NaF. The 20-minute study showed that there was no selective affinity for particular ions in camel or bovine corneal stroma. Initial swelling in both corneal and bovine stroma is faster and more prominant compared to later swelling. The swelling in camel cornea is more prominant compared to bovine corneal stroma. This could be due to higher negatively charged keratin sulfate&ndash;proteoglycans in the stroma. Lower rehydration in camel cornea suggests stronger leaching of proteoglycans from stroma in NaF.Keywords: camel, swelling, sodium thiocyanate, sodium bicarbonate, proteoglycans Turki AlmubradMohammad Faisal Jamal KhanSaeed AkhtarDove Medical PressarticleOphthalmologyRE1-994ENClinical Ophthalmology, Vol 2010, Iss default, Pp 1053-1060 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ophthalmology RE1-994
spellingShingle	Ophthalmology RE1-994 Turki Almubrad Mohammad Faisal Jamal Khan Saeed Akhtar Swelling studies of camel and bovine corneal stroma
description	Turki Almubrad, Mohammad Faisal Jamal Khan, Saeed AkhtarCornea Research Chair, Department of Optometry, College of Applied Medical Sciences, King Saud University, Saudi ArabiaAbstract: In the present study we investigated the swelling characteristics of fresh camel and bovine cornea in sodium salt solutions. Swelling studies were carried out at 20 minutes, 14 hours, and 46 hours on five fresh camel and 5 five fresh bovine corneas. During the 20-minute hydration of fresh corneal stroma was investigated using sodium chloride (NaCl), sodium bicarbonate (NaHCO3), sodium acetate (CH3COONa), sodium thiocyanate (NaSCN), and sodium floride (NaF) at 2-minute time intervals. During a 46-hour time period, the hydration study was carried out using NaCl (150, 300 mM) and NaF (150 mM) at random intervals. The 14-hour study was carried out to assess the rehydration of corneal stroma after 6 hours of drying. During the 20-minute swelling studies in the first 2 minutes the rate of hydration in both camel and bovine corneas was high but gradually reduced in the 2&ndash;20-minute period. The rates and levels of hydration of camel and bovine cornea were not significantly different from each other in all the strengths of solutions. During the 46-hour swelling studies, the initial rate of hydration (0&ndash;2 hours) of camel and bovine stroma, in all solutions was significantly higher (Z = 0.056) compared to hydration during later hours (2&ndash;46 hours). Camel stromal hydration (high) in 150 mM NaCl was significantly higher compared to bovine stromal hydration in the same solution during the 10&ndash;24, and 24&ndash;46-hour time periods. Rehydration in camel stroma was significantly lower than bovine in 150 mM NaF. The 20-minute study showed that there was no selective affinity for particular ions in camel or bovine corneal stroma. Initial swelling in both corneal and bovine stroma is faster and more prominant compared to later swelling. The swelling in camel cornea is more prominant compared to bovine corneal stroma. This could be due to higher negatively charged keratin sulfate&ndash;proteoglycans in the stroma. Lower rehydration in camel cornea suggests stronger leaching of proteoglycans from stroma in NaF.Keywords: camel, swelling, sodium thiocyanate, sodium bicarbonate, proteoglycans
format	article
author	Turki Almubrad Mohammad Faisal Jamal Khan Saeed Akhtar
author_facet	Turki Almubrad Mohammad Faisal Jamal Khan Saeed Akhtar
author_sort	Turki Almubrad
title	Swelling studies of camel and bovine corneal stroma
title_short	Swelling studies of camel and bovine corneal stroma
title_full	Swelling studies of camel and bovine corneal stroma
title_fullStr	Swelling studies of camel and bovine corneal stroma
title_full_unstemmed	Swelling studies of camel and bovine corneal stroma
title_sort	swelling studies of camel and bovine corneal stroma
publisher	Dove Medical Press
publishDate	2010
url	https://doaj.org/article/980c5bac012449f5bd62182f6e89c0d8
work_keys_str_mv	AT turkialmubrad swellingstudiesofcamelandbovinecornealstroma AT mohammadfaisaljamalkhan swellingstudiesofcamelandbovinecornealstroma AT saeedakhtar swellingstudiesofcamelandbovinecornealstroma
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Swelling studies of camel and bovine corneal stroma

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