Host-Pathogen Interactions: What the EHEC Are We Learning from Host Genome-Wide Screens?

ABSTRACT Several genome-wide screens have been conducted to identify host cell factors involved in the pathogenesis of bacterial pathogens whose virulence is dependent on type III secretion systems (T3SSs), nanomachines responsible for the translocation of proteins into host cells. In the most recen...

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Autores principales:	Jason P. Lynch, Cammie F. Lesser
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	EHEC Shigella Vibrio parahaemolyticus CRISPR/Cas9 screen type III secretion system Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/9815bd43629b4cf0ab4abe01ca6b9a90
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spelling	oai:doaj.org-article:9815bd43629b4cf0ab4abe01ca6b9a902021-11-15T15:58:21ZHost-Pathogen Interactions: What the EHEC Are We Learning from Host Genome-Wide Screens?10.1128/mBio.01837-182150-7511https://doaj.org/article/9815bd43629b4cf0ab4abe01ca6b9a902018-11-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.01837-18https://doaj.org/toc/2150-7511ABSTRACT Several genome-wide screens have been conducted to identify host cell factors involved in the pathogenesis of bacterial pathogens whose virulence is dependent on type III secretion systems (T3SSs), nanomachines responsible for the translocation of proteins into host cells. In the most recent of these, Pacheco et al. (mBio 9:e01003-18, 2018, http://mbio.asm.org/content/9/3/e01003-18.full) screened a genome-wide CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats with Cas9) knockout library for host proteins involved in the pathogenesis of enterohemorrhagic Escherichia coli (EHEC). Their study revealed an unrecognized link between EHEC’s two major virulence determinants (its T3SS and Shiga toxins). We discuss these findings in light of data from three other genome-wide screens. Each of these studies uncovered multiple host cell determinants, which curiously share little to no overlap but primarily are involved in mediating early interactions between T3SSs and host cells. We therefore consider how each screen was performed, the advantages and disadvantages of each, and how follow-up studies might be designed to address these issues.Jason P. LynchCammie F. LesserAmerican Society for MicrobiologyarticleEHECShigellaVibrio parahaemolyticusCRISPR/Cas9 screentype III secretion systemMicrobiologyQR1-502ENmBio, Vol 9, Iss 5 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	EHEC Shigella Vibrio parahaemolyticus CRISPR/Cas9 screen type III secretion system Microbiology QR1-502
spellingShingle	EHEC Shigella Vibrio parahaemolyticus CRISPR/Cas9 screen type III secretion system Microbiology QR1-502 Jason P. Lynch Cammie F. Lesser Host-Pathogen Interactions: What the EHEC Are We Learning from Host Genome-Wide Screens?
description	ABSTRACT Several genome-wide screens have been conducted to identify host cell factors involved in the pathogenesis of bacterial pathogens whose virulence is dependent on type III secretion systems (T3SSs), nanomachines responsible for the translocation of proteins into host cells. In the most recent of these, Pacheco et al. (mBio 9:e01003-18, 2018, http://mbio.asm.org/content/9/3/e01003-18.full) screened a genome-wide CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats with Cas9) knockout library for host proteins involved in the pathogenesis of enterohemorrhagic Escherichia coli (EHEC). Their study revealed an unrecognized link between EHEC’s two major virulence determinants (its T3SS and Shiga toxins). We discuss these findings in light of data from three other genome-wide screens. Each of these studies uncovered multiple host cell determinants, which curiously share little to no overlap but primarily are involved in mediating early interactions between T3SSs and host cells. We therefore consider how each screen was performed, the advantages and disadvantages of each, and how follow-up studies might be designed to address these issues.
format	article
author	Jason P. Lynch Cammie F. Lesser
author_facet	Jason P. Lynch Cammie F. Lesser
author_sort	Jason P. Lynch
title	Host-Pathogen Interactions: What the EHEC Are We Learning from Host Genome-Wide Screens?
title_short	Host-Pathogen Interactions: What the EHEC Are We Learning from Host Genome-Wide Screens?
title_full	Host-Pathogen Interactions: What the EHEC Are We Learning from Host Genome-Wide Screens?
title_fullStr	Host-Pathogen Interactions: What the EHEC Are We Learning from Host Genome-Wide Screens?
title_full_unstemmed	Host-Pathogen Interactions: What the EHEC Are We Learning from Host Genome-Wide Screens?
title_sort	host-pathogen interactions: what the ehec are we learning from host genome-wide screens?
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/9815bd43629b4cf0ab4abe01ca6b9a90
work_keys_str_mv	AT jasonplynch hostpathogeninteractionswhattheehecarewelearningfromhostgenomewidescreens AT cammieflesser hostpathogeninteractionswhattheehecarewelearningfromhostgenomewidescreens
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Host-Pathogen Interactions: What the EHEC Are We Learning from Host Genome-Wide Screens?

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