Interactions and CCAAT-binding of Arabidopsis thaliana NF-Y subunits.

<h4>Background</h4>NF-Y is a transcription factor that recognizes with high specificity and affinity the widespread CCAAT box promoter element. It is formed by three subunits: NF-YA and the NF-YB/NF-YC- heterodimer containing histone fold domains (HFDs). We previously identified a large...

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Autores principales:	Valentina Calvenzani, Barbara Testoni, Giuliana Gusmaroli, Mariangela Lorenzo, Nerina Gnesutta, Katia Petroni, Roberto Mantovani, Chiara Tonelli
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9817d0e86a104eee8b8b6c6b515de365
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spelling	oai:doaj.org-article:9817d0e86a104eee8b8b6c6b515de3652021-11-18T07:08:28ZInteractions and CCAAT-binding of Arabidopsis thaliana NF-Y subunits.1932-620310.1371/journal.pone.0042902https://doaj.org/article/9817d0e86a104eee8b8b6c6b515de3652012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22912760/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>NF-Y is a transcription factor that recognizes with high specificity and affinity the widespread CCAAT box promoter element. It is formed by three subunits: NF-YA and the NF-YB/NF-YC- heterodimer containing histone fold domains (HFDs). We previously identified a large NF-Y gene family in Arabidopsis thaliana, composed of 29 members, and characterized their expression patterns in various plant tissues.<h4>Methods</h4>We used yeast Two-hybrids assays (Y2H), pull-down and Electrophoretic Mobility Shift Assay (EMSA) in vitro experiments with recombinant proteins to dissect AtNF-YB/AtNF-YC interactions and DNA-binding with different AtNF-YAs.<h4>Results</h4>Consistent with robust conservation within HFDs, we show that heterodimerization is possible among all histone-like subunits, including the divergent and related LEC1/AtNF-YB9 and L1L/AtNF-YB6 required for embryo development. DNA-binding to a consensus CCAAT box was investigated with specific AtNF-YB/AtNF-YC combinations and observed with some, but not all AtNF-YA subunits.<h4>Conclusions</h4>Our results highlight (i) the conserved heterodimerization capacity of AtNF-Y histone-like subunits, and (ii) the different affinities of AtNF-YAs for the CCAAT sequence. Because of the general expansion of NF-Y genes in plants, these results most likely apply to other species.Valentina CalvenzaniBarbara TestoniGiuliana GusmaroliMariangela LorenzoNerina GnesuttaKatia PetroniRoberto MantovaniChiara TonelliPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 8, p e42902 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Valentina Calvenzani Barbara Testoni Giuliana Gusmaroli Mariangela Lorenzo Nerina Gnesutta Katia Petroni Roberto Mantovani Chiara Tonelli Interactions and CCAAT-binding of Arabidopsis thaliana NF-Y subunits.
description	<h4>Background</h4>NF-Y is a transcription factor that recognizes with high specificity and affinity the widespread CCAAT box promoter element. It is formed by three subunits: NF-YA and the NF-YB/NF-YC- heterodimer containing histone fold domains (HFDs). We previously identified a large NF-Y gene family in Arabidopsis thaliana, composed of 29 members, and characterized their expression patterns in various plant tissues.<h4>Methods</h4>We used yeast Two-hybrids assays (Y2H), pull-down and Electrophoretic Mobility Shift Assay (EMSA) in vitro experiments with recombinant proteins to dissect AtNF-YB/AtNF-YC interactions and DNA-binding with different AtNF-YAs.<h4>Results</h4>Consistent with robust conservation within HFDs, we show that heterodimerization is possible among all histone-like subunits, including the divergent and related LEC1/AtNF-YB9 and L1L/AtNF-YB6 required for embryo development. DNA-binding to a consensus CCAAT box was investigated with specific AtNF-YB/AtNF-YC combinations and observed with some, but not all AtNF-YA subunits.<h4>Conclusions</h4>Our results highlight (i) the conserved heterodimerization capacity of AtNF-Y histone-like subunits, and (ii) the different affinities of AtNF-YAs for the CCAAT sequence. Because of the general expansion of NF-Y genes in plants, these results most likely apply to other species.
format	article
author	Valentina Calvenzani Barbara Testoni Giuliana Gusmaroli Mariangela Lorenzo Nerina Gnesutta Katia Petroni Roberto Mantovani Chiara Tonelli
author_facet	Valentina Calvenzani Barbara Testoni Giuliana Gusmaroli Mariangela Lorenzo Nerina Gnesutta Katia Petroni Roberto Mantovani Chiara Tonelli
author_sort	Valentina Calvenzani
title	Interactions and CCAAT-binding of Arabidopsis thaliana NF-Y subunits.
title_short	Interactions and CCAAT-binding of Arabidopsis thaliana NF-Y subunits.
title_full	Interactions and CCAAT-binding of Arabidopsis thaliana NF-Y subunits.
title_fullStr	Interactions and CCAAT-binding of Arabidopsis thaliana NF-Y subunits.
title_full_unstemmed	Interactions and CCAAT-binding of Arabidopsis thaliana NF-Y subunits.
title_sort	interactions and ccaat-binding of arabidopsis thaliana nf-y subunits.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/9817d0e86a104eee8b8b6c6b515de365
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Interactions and CCAAT-binding of Arabidopsis thaliana NF-Y subunits.

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