Proteomic analysis of proton beam irradiated human melanoma cells.

Proton beam irradiation is a form of advanced radiotherapy providing superior distributions of a low LET radiation dose relative to that of photon therapy for the treatment of cancer. Even though this clinical treatment has been developing for several decades, the proton radiobiology critical to the...

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Autores principales:	Sylwia Kedracka-Krok, Urszula Jankowska, Martyna Elas, Urszula Sowa, Jan Swakon, Agnieszka Cierniak, Pawel Olko, Bozena Romanowska-Dixon, Krystyna Urbanska
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/98198cbf43944da69b46d5f2db694a61
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spelling	oai:doaj.org-article:98198cbf43944da69b46d5f2db694a612021-11-18T08:39:04ZProteomic analysis of proton beam irradiated human melanoma cells.1932-620310.1371/journal.pone.0084621https://doaj.org/article/98198cbf43944da69b46d5f2db694a612014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24392146/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Proton beam irradiation is a form of advanced radiotherapy providing superior distributions of a low LET radiation dose relative to that of photon therapy for the treatment of cancer. Even though this clinical treatment has been developing for several decades, the proton radiobiology critical to the optimization of proton radiotherapy is far from being understood. Proteomic changes were analyzed in human melanoma cells treated with a sublethal dose (3 Gy) of proton beam irradiation. The results were compared with untreated cells. Two-dimensional electrophoresis was performed with mass spectrometry to identify the proteins. At the dose of 3 Gy a minimal slowdown in proliferation rate was seen, as well as some DNA damage. After allowing time for damage repair, the proteomic analysis was performed. In total 17 protein levels were found to significantly (more than 1.5 times) change: 4 downregulated and 13 upregulated. Functionally, they represent four categories: (i) DNA repair and RNA regulation (VCP, MVP, STRAP, FAB-2, Lamine A/C, GAPDH), (ii) cell survival and stress response (STRAP, MCM7, Annexin 7, MVP, Caprin-1, PDCD6, VCP, HSP70), (iii) cell metabolism (TIM, GAPDH, VCP), and (iv) cytoskeleton and motility (Moesin, Actinin 4, FAB-2, Vimentin, Annexin 7, Lamine A/C, Lamine B). A substantial decrease (2.3 x) was seen in the level of vimentin, a marker of epithelial to mesenchymal transition and the metastatic properties of melanoma.Sylwia Kedracka-KrokUrszula JankowskaMartyna ElasUrszula SowaJan SwakonAgnieszka CierniakPawel OlkoBozena Romanowska-DixonKrystyna UrbanskaPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e84621 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sylwia Kedracka-Krok Urszula Jankowska Martyna Elas Urszula Sowa Jan Swakon Agnieszka Cierniak Pawel Olko Bozena Romanowska-Dixon Krystyna Urbanska Proteomic analysis of proton beam irradiated human melanoma cells.
description	Proton beam irradiation is a form of advanced radiotherapy providing superior distributions of a low LET radiation dose relative to that of photon therapy for the treatment of cancer. Even though this clinical treatment has been developing for several decades, the proton radiobiology critical to the optimization of proton radiotherapy is far from being understood. Proteomic changes were analyzed in human melanoma cells treated with a sublethal dose (3 Gy) of proton beam irradiation. The results were compared with untreated cells. Two-dimensional electrophoresis was performed with mass spectrometry to identify the proteins. At the dose of 3 Gy a minimal slowdown in proliferation rate was seen, as well as some DNA damage. After allowing time for damage repair, the proteomic analysis was performed. In total 17 protein levels were found to significantly (more than 1.5 times) change: 4 downregulated and 13 upregulated. Functionally, they represent four categories: (i) DNA repair and RNA regulation (VCP, MVP, STRAP, FAB-2, Lamine A/C, GAPDH), (ii) cell survival and stress response (STRAP, MCM7, Annexin 7, MVP, Caprin-1, PDCD6, VCP, HSP70), (iii) cell metabolism (TIM, GAPDH, VCP), and (iv) cytoskeleton and motility (Moesin, Actinin 4, FAB-2, Vimentin, Annexin 7, Lamine A/C, Lamine B). A substantial decrease (2.3 x) was seen in the level of vimentin, a marker of epithelial to mesenchymal transition and the metastatic properties of melanoma.
format	article
author	Sylwia Kedracka-Krok Urszula Jankowska Martyna Elas Urszula Sowa Jan Swakon Agnieszka Cierniak Pawel Olko Bozena Romanowska-Dixon Krystyna Urbanska
author_facet	Sylwia Kedracka-Krok Urszula Jankowska Martyna Elas Urszula Sowa Jan Swakon Agnieszka Cierniak Pawel Olko Bozena Romanowska-Dixon Krystyna Urbanska
author_sort	Sylwia Kedracka-Krok
title	Proteomic analysis of proton beam irradiated human melanoma cells.
title_short	Proteomic analysis of proton beam irradiated human melanoma cells.
title_full	Proteomic analysis of proton beam irradiated human melanoma cells.
title_fullStr	Proteomic analysis of proton beam irradiated human melanoma cells.
title_full_unstemmed	Proteomic analysis of proton beam irradiated human melanoma cells.
title_sort	proteomic analysis of proton beam irradiated human melanoma cells.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/98198cbf43944da69b46d5f2db694a61
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Proteomic analysis of proton beam irradiated human melanoma cells.

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