Droplet Digital PCR for <i>BCR–ABL1</i> Monitoring in Diagnostic Routine: Ready to Start?
<i>BCR–ABL1</i> mRNA levels represent the key molecular marker for the evaluation of minimal residual disease (MRD) in chronic myeloid leukemia (CML) patients and real-time quantitative PCR (RT-qPCR) is currently the standard method to monitor it. In the era of tyrosine kinase inhibitors...
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MDPI AG
2021
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oai:doaj.org-article:9821f3a48f02453b8ebd95e92daef6e32021-11-11T15:33:36ZDroplet Digital PCR for <i>BCR–ABL1</i> Monitoring in Diagnostic Routine: Ready to Start?10.3390/cancers132154702072-6694https://doaj.org/article/9821f3a48f02453b8ebd95e92daef6e32021-10-01T00:00:00Zhttps://www.mdpi.com/2072-6694/13/21/5470https://doaj.org/toc/2072-6694<i>BCR–ABL1</i> mRNA levels represent the key molecular marker for the evaluation of minimal residual disease (MRD) in chronic myeloid leukemia (CML) patients and real-time quantitative PCR (RT-qPCR) is currently the standard method to monitor it. In the era of tyrosine kinase inhibitors (TKIs) discontinuation, droplet digital PCR (ddPCR) has emerged to provide a more precise detection of MRD. To hypothesize the use of ddPCR in clinical practice, we designed a multicentric study to evaluate the potential value of ddPCR in the diagnostic routine. Thirty-seven RNA samples from CML patients and five from healthy donors were analyzed using both ddPCR QXDx<sup>TM</sup><i>BCR-ABL</i> %IS Kit and LabNet-approved RT-qPCR methodologies in three different Italian laboratories. Our results show that ddPCR has a good agreement with RT-qPCR, but it is more precise to quantify <i>BCR–ABL1</i> transcript levels. Furthermore, we did not find differences between duplicate or quadruplicate analysis in terms of <i>BCR–ABL1</i>% IS values. Droplet digital PCR could be confidently introduced into the diagnostic routine as a complement to the RT-qPCR.Maria Teresa BochicchioJessica PetitiPaola BerchiallaBarbara IzzoEmilia GiuglianoEmanuela OttavianiSanta ErrichielloGiovanna Rege-CambrinClaudia VenturiLuigiana LucianoFilomena DaraioDaniele CalistriGianantonio RostiGiuseppe SaglioGiovanni MartinelliFabrizio PaneDaniela CilloniEnrico M. GottardiCarmen FavaMDPI AGarticlechronic myeloid leukemiaddPCR<i>BCR–ABL1</i>deep molecular responsetreatment-free remissionNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENCancers, Vol 13, Iss 5470, p 5470 (2021) |
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DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
chronic myeloid leukemia ddPCR <i>BCR–ABL1</i> deep molecular response treatment-free remission Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 |
| spellingShingle |
chronic myeloid leukemia ddPCR <i>BCR–ABL1</i> deep molecular response treatment-free remission Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Maria Teresa Bochicchio Jessica Petiti Paola Berchialla Barbara Izzo Emilia Giugliano Emanuela Ottaviani Santa Errichiello Giovanna Rege-Cambrin Claudia Venturi Luigiana Luciano Filomena Daraio Daniele Calistri Gianantonio Rosti Giuseppe Saglio Giovanni Martinelli Fabrizio Pane Daniela Cilloni Enrico M. Gottardi Carmen Fava Droplet Digital PCR for <i>BCR–ABL1</i> Monitoring in Diagnostic Routine: Ready to Start? |
| description |
<i>BCR–ABL1</i> mRNA levels represent the key molecular marker for the evaluation of minimal residual disease (MRD) in chronic myeloid leukemia (CML) patients and real-time quantitative PCR (RT-qPCR) is currently the standard method to monitor it. In the era of tyrosine kinase inhibitors (TKIs) discontinuation, droplet digital PCR (ddPCR) has emerged to provide a more precise detection of MRD. To hypothesize the use of ddPCR in clinical practice, we designed a multicentric study to evaluate the potential value of ddPCR in the diagnostic routine. Thirty-seven RNA samples from CML patients and five from healthy donors were analyzed using both ddPCR QXDx<sup>TM</sup><i>BCR-ABL</i> %IS Kit and LabNet-approved RT-qPCR methodologies in three different Italian laboratories. Our results show that ddPCR has a good agreement with RT-qPCR, but it is more precise to quantify <i>BCR–ABL1</i> transcript levels. Furthermore, we did not find differences between duplicate or quadruplicate analysis in terms of <i>BCR–ABL1</i>% IS values. Droplet digital PCR could be confidently introduced into the diagnostic routine as a complement to the RT-qPCR. |
| format |
article |
| author |
Maria Teresa Bochicchio Jessica Petiti Paola Berchialla Barbara Izzo Emilia Giugliano Emanuela Ottaviani Santa Errichiello Giovanna Rege-Cambrin Claudia Venturi Luigiana Luciano Filomena Daraio Daniele Calistri Gianantonio Rosti Giuseppe Saglio Giovanni Martinelli Fabrizio Pane Daniela Cilloni Enrico M. Gottardi Carmen Fava |
| author_facet |
Maria Teresa Bochicchio Jessica Petiti Paola Berchialla Barbara Izzo Emilia Giugliano Emanuela Ottaviani Santa Errichiello Giovanna Rege-Cambrin Claudia Venturi Luigiana Luciano Filomena Daraio Daniele Calistri Gianantonio Rosti Giuseppe Saglio Giovanni Martinelli Fabrizio Pane Daniela Cilloni Enrico M. Gottardi Carmen Fava |
| author_sort |
Maria Teresa Bochicchio |
| title |
Droplet Digital PCR for <i>BCR–ABL1</i> Monitoring in Diagnostic Routine: Ready to Start? |
| title_short |
Droplet Digital PCR for <i>BCR–ABL1</i> Monitoring in Diagnostic Routine: Ready to Start? |
| title_full |
Droplet Digital PCR for <i>BCR–ABL1</i> Monitoring in Diagnostic Routine: Ready to Start? |
| title_fullStr |
Droplet Digital PCR for <i>BCR–ABL1</i> Monitoring in Diagnostic Routine: Ready to Start? |
| title_full_unstemmed |
Droplet Digital PCR for <i>BCR–ABL1</i> Monitoring in Diagnostic Routine: Ready to Start? |
| title_sort |
droplet digital pcr for <i>bcr–abl1</i> monitoring in diagnostic routine: ready to start? |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/9821f3a48f02453b8ebd95e92daef6e3 |
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