HIV tropism switch in archived DNA of HIV-HCV subjects successfully treated with direct-acting antivirals for HCV infection

Abstract We described short-term HIV tropism changes occurring in peripheral blood mononuclear cells and the correlations with HIV DNA value in HIV-HCV co-infected patients cured for HCV disease and with undetectable HIV viremia or residual viremia (RV). Plasma HIV RNA, cellular HIV DNA and tropism...

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Autores principales:	Monica Basso, Daniela Zago, Renzo Scaggiante, Silvia Cavinato, Irene Pozzetto, Camilla Stagni, Beatrice Parisatto, Anna Maria Cattelan, Giuliana Battagin, Loredana Sarmati, Saverio Giuseppe Parisi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9834c452790448a594eb5270b459afda
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spelling	oai:doaj.org-article:9834c452790448a594eb5270b459afda2021-12-02T16:55:46ZHIV tropism switch in archived DNA of HIV-HCV subjects successfully treated with direct-acting antivirals for HCV infection10.1038/s41598-021-88811-62045-2322https://doaj.org/article/9834c452790448a594eb5270b459afda2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88811-6https://doaj.org/toc/2045-2322Abstract We described short-term HIV tropism changes occurring in peripheral blood mononuclear cells and the correlations with HIV DNA value in HIV-HCV co-infected patients cured for HCV disease and with undetectable HIV viremia or residual viremia (RV). Plasma HIV RNA, cellular HIV DNA and tropism were evaluated pre-HCV treatment (baseline, BL) and at 12(T1) and 24(T2) weeks after HCV treatment start. V3 sequences were interpreted using Geno2pheno and classified as R5 only if all three sequences had an FPR ≥ 10% and as X4 when at least one replicate sequence had an FPR < 10%. Forty-nine patients (21 with X4 and 28 with R5 virus) were enrolled. Five X4 patients and 9 R5 subjects experienced at least one tropism change,11 with RV:1/5 patients with X4 infection at BL switched at T1 versus 8/9 in the R5 group (p = 0.022977) and the difference was confirmed in subjects with RV (p = 0.02);6/9 R5 patients switching at T1 confirmed the tropism change at T2. No significant differences in HIV DNA values between patients with RV starting with a R5 or X4 tropism and experienced tropism switch or not were found. Short-term tropism switch involved almost a third of patients, in all but three cases with HIV RV. Being R5 at BL is associated to a higher instability, expressed as number of tropism changes and confirmed switch at T2.Monica BassoDaniela ZagoRenzo ScaggianteSilvia CavinatoIrene PozzettoCamilla StagniBeatrice ParisattoAnna Maria CattelanGiuliana BattaginLoredana SarmatiSaverio Giuseppe ParisiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Monica Basso Daniela Zago Renzo Scaggiante Silvia Cavinato Irene Pozzetto Camilla Stagni Beatrice Parisatto Anna Maria Cattelan Giuliana Battagin Loredana Sarmati Saverio Giuseppe Parisi HIV tropism switch in archived DNA of HIV-HCV subjects successfully treated with direct-acting antivirals for HCV infection
description	Abstract We described short-term HIV tropism changes occurring in peripheral blood mononuclear cells and the correlations with HIV DNA value in HIV-HCV co-infected patients cured for HCV disease and with undetectable HIV viremia or residual viremia (RV). Plasma HIV RNA, cellular HIV DNA and tropism were evaluated pre-HCV treatment (baseline, BL) and at 12(T1) and 24(T2) weeks after HCV treatment start. V3 sequences were interpreted using Geno2pheno and classified as R5 only if all three sequences had an FPR ≥ 10% and as X4 when at least one replicate sequence had an FPR < 10%. Forty-nine patients (21 with X4 and 28 with R5 virus) were enrolled. Five X4 patients and 9 R5 subjects experienced at least one tropism change,11 with RV:1/5 patients with X4 infection at BL switched at T1 versus 8/9 in the R5 group (p = 0.022977) and the difference was confirmed in subjects with RV (p = 0.02);6/9 R5 patients switching at T1 confirmed the tropism change at T2. No significant differences in HIV DNA values between patients with RV starting with a R5 or X4 tropism and experienced tropism switch or not were found. Short-term tropism switch involved almost a third of patients, in all but three cases with HIV RV. Being R5 at BL is associated to a higher instability, expressed as number of tropism changes and confirmed switch at T2.
format	article
author	Monica Basso Daniela Zago Renzo Scaggiante Silvia Cavinato Irene Pozzetto Camilla Stagni Beatrice Parisatto Anna Maria Cattelan Giuliana Battagin Loredana Sarmati Saverio Giuseppe Parisi
author_facet	Monica Basso Daniela Zago Renzo Scaggiante Silvia Cavinato Irene Pozzetto Camilla Stagni Beatrice Parisatto Anna Maria Cattelan Giuliana Battagin Loredana Sarmati Saverio Giuseppe Parisi
author_sort	Monica Basso
title	HIV tropism switch in archived DNA of HIV-HCV subjects successfully treated with direct-acting antivirals for HCV infection
title_short	HIV tropism switch in archived DNA of HIV-HCV subjects successfully treated with direct-acting antivirals for HCV infection
title_full	HIV tropism switch in archived DNA of HIV-HCV subjects successfully treated with direct-acting antivirals for HCV infection
title_fullStr	HIV tropism switch in archived DNA of HIV-HCV subjects successfully treated with direct-acting antivirals for HCV infection
title_full_unstemmed	HIV tropism switch in archived DNA of HIV-HCV subjects successfully treated with direct-acting antivirals for HCV infection
title_sort	hiv tropism switch in archived dna of hiv-hcv subjects successfully treated with direct-acting antivirals for hcv infection
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9834c452790448a594eb5270b459afda
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HIV tropism switch in archived DNA of HIV-HCV subjects successfully treated with direct-acting antivirals for HCV infection

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