The Long Non-Coding RNA SAMMSON Is a Regulator of Chemosensitivity and Metabolic Orientation in MCF-7 Doxorubicin-Resistant Breast Cancer Cells

Despite improvements in therapeutic strategies for treating breast cancers, tumor relapse and chemoresistance remain major issues in patient outcomes. Indeed, cancer cells display a metabolic plasticity allowing a quick adaptation to the tumoral microenvironment and to cellular stresses induced by c...

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Autores principales:	Charlotte Orre, Xavier Dieu, Jordan Guillon, Naïg Gueguen, Seyedeh Tayebeh Ahmadpour, Jean-François Dumas, Salim Khiati, Pascal Reynier, Guy Lenaers, Olivier Coqueret, Arnaud Chevrollier, Delphine Mirebeau-Prunier, Valérie Desquiret-Dumas
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	breast cancer mitochondria metabolism complex I long non-coding RNA SAMMSON Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/98353807760346749bdef56025f7443a
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spelling	oai:doaj.org-article:98353807760346749bdef56025f7443a2021-11-25T16:47:32ZThe Long Non-Coding RNA SAMMSON Is a Regulator of Chemosensitivity and Metabolic Orientation in MCF-7 Doxorubicin-Resistant Breast Cancer Cells10.3390/biology101111562079-7737https://doaj.org/article/98353807760346749bdef56025f7443a2021-11-01T00:00:00Zhttps://www.mdpi.com/2079-7737/10/11/1156https://doaj.org/toc/2079-7737Despite improvements in therapeutic strategies for treating breast cancers, tumor relapse and chemoresistance remain major issues in patient outcomes. Indeed, cancer cells display a metabolic plasticity allowing a quick adaptation to the tumoral microenvironment and to cellular stresses induced by chemotherapy. Recently, long non-coding RNA molecules (lncRNAs) have emerged as important regulators of cellular metabolic orientation. In the present study, we addressed the role of the long non-coding RNA molecule (lncRNA) SAMMSON on the metabolic reprogramming and chemoresistance of MCF-7 breast cancer cells resistant to doxorubicin (MCF-7dox). Our results showed an overexpression of SAMMSON in MCF-7dox compared to doxorubicin-sensitive cells (MCF-7). Silencing of SAMMSON expression by siRNA in MCF-7dox cells resulted in a metabolic rewiring with improvement of oxidative metabolism, decreased mitochondrial ROS production, increased mitochondrial replication, transcription and translation and an attenuation of chemoresistance. These results highlight the role of SAMMSON in the metabolic adaptations leading to the development of chemoresistance in breast cancer cells. Thus, targeting SAMMSON expression levels represents a promising therapeutic route to circumvent doxorubicin resistance in breast cancers.Charlotte OrreXavier DieuJordan GuillonNaïg GueguenSeyedeh Tayebeh AhmadpourJean-François DumasSalim KhiatiPascal ReynierGuy LenaersOlivier CoqueretArnaud ChevrollierDelphine Mirebeau-PrunierValérie Desquiret-DumasMDPI AGarticlebreast cancermitochondriametabolismcomplex Ilong non-coding RNASAMMSONBiology (General)QH301-705.5ENBiology, Vol 10, Iss 1156, p 1156 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	breast cancer mitochondria metabolism complex I long non-coding RNA SAMMSON Biology (General) QH301-705.5
spellingShingle	breast cancer mitochondria metabolism complex I long non-coding RNA SAMMSON Biology (General) QH301-705.5 Charlotte Orre Xavier Dieu Jordan Guillon Naïg Gueguen Seyedeh Tayebeh Ahmadpour Jean-François Dumas Salim Khiati Pascal Reynier Guy Lenaers Olivier Coqueret Arnaud Chevrollier Delphine Mirebeau-Prunier Valérie Desquiret-Dumas The Long Non-Coding RNA SAMMSON Is a Regulator of Chemosensitivity and Metabolic Orientation in MCF-7 Doxorubicin-Resistant Breast Cancer Cells
description	Despite improvements in therapeutic strategies for treating breast cancers, tumor relapse and chemoresistance remain major issues in patient outcomes. Indeed, cancer cells display a metabolic plasticity allowing a quick adaptation to the tumoral microenvironment and to cellular stresses induced by chemotherapy. Recently, long non-coding RNA molecules (lncRNAs) have emerged as important regulators of cellular metabolic orientation. In the present study, we addressed the role of the long non-coding RNA molecule (lncRNA) SAMMSON on the metabolic reprogramming and chemoresistance of MCF-7 breast cancer cells resistant to doxorubicin (MCF-7dox). Our results showed an overexpression of SAMMSON in MCF-7dox compared to doxorubicin-sensitive cells (MCF-7). Silencing of SAMMSON expression by siRNA in MCF-7dox cells resulted in a metabolic rewiring with improvement of oxidative metabolism, decreased mitochondrial ROS production, increased mitochondrial replication, transcription and translation and an attenuation of chemoresistance. These results highlight the role of SAMMSON in the metabolic adaptations leading to the development of chemoresistance in breast cancer cells. Thus, targeting SAMMSON expression levels represents a promising therapeutic route to circumvent doxorubicin resistance in breast cancers.
format	article
author	Charlotte Orre Xavier Dieu Jordan Guillon Naïg Gueguen Seyedeh Tayebeh Ahmadpour Jean-François Dumas Salim Khiati Pascal Reynier Guy Lenaers Olivier Coqueret Arnaud Chevrollier Delphine Mirebeau-Prunier Valérie Desquiret-Dumas
author_facet	Charlotte Orre Xavier Dieu Jordan Guillon Naïg Gueguen Seyedeh Tayebeh Ahmadpour Jean-François Dumas Salim Khiati Pascal Reynier Guy Lenaers Olivier Coqueret Arnaud Chevrollier Delphine Mirebeau-Prunier Valérie Desquiret-Dumas
author_sort	Charlotte Orre
title	The Long Non-Coding RNA SAMMSON Is a Regulator of Chemosensitivity and Metabolic Orientation in MCF-7 Doxorubicin-Resistant Breast Cancer Cells
title_short	The Long Non-Coding RNA SAMMSON Is a Regulator of Chemosensitivity and Metabolic Orientation in MCF-7 Doxorubicin-Resistant Breast Cancer Cells
title_full	The Long Non-Coding RNA SAMMSON Is a Regulator of Chemosensitivity and Metabolic Orientation in MCF-7 Doxorubicin-Resistant Breast Cancer Cells
title_fullStr	The Long Non-Coding RNA SAMMSON Is a Regulator of Chemosensitivity and Metabolic Orientation in MCF-7 Doxorubicin-Resistant Breast Cancer Cells
title_full_unstemmed	The Long Non-Coding RNA SAMMSON Is a Regulator of Chemosensitivity and Metabolic Orientation in MCF-7 Doxorubicin-Resistant Breast Cancer Cells
title_sort	long non-coding rna sammson is a regulator of chemosensitivity and metabolic orientation in mcf-7 doxorubicin-resistant breast cancer cells
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/98353807760346749bdef56025f7443a
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The Long Non-Coding RNA SAMMSON Is a Regulator of Chemosensitivity and Metabolic Orientation in MCF-7 Doxorubicin-Resistant Breast Cancer Cells

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