Molecular epidemiology of methicillin resistant Staphylococcus species in healthcare workers of a blood bank in the Brazilian Amazon

Abstract Background Healthcare workers are susceptible to colonization by multiresistant bacteria, which can increase the risk of outbreaks. Methods Samples were collected from the nasopharynx, hands, and lab coats of healthcare workers. The phenotypic identification was carried out using a VITEK®2...

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Autores principales:	Cristina Motta Ferreira, Roberto Alexandre Alves Barbosa Filho, Guilherme Motta Antunes Ferreira, Marcus Vinicius Guimarães de Lacerda, Cintia Mara Costa de Oliveira, Vanderson de Souza Sampaio, Lucyane Mendes Silva, Andreza Gomes Pascoal, William Antunes Ferreira
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	S. epidermidis Multirresistance Oxacillin ST Clone Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/98368a6af62a446fb88c71c311ff49cb
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spelling	oai:doaj.org-article:98368a6af62a446fb88c71c311ff49cb2021-11-07T12:08:59ZMolecular epidemiology of methicillin resistant Staphylococcus species in healthcare workers of a blood bank in the Brazilian Amazon10.1186/s12866-021-02365-11471-2180https://doaj.org/article/98368a6af62a446fb88c71c311ff49cb2021-11-01T00:00:00Zhttps://doi.org/10.1186/s12866-021-02365-1https://doaj.org/toc/1471-2180Abstract Background Healthcare workers are susceptible to colonization by multiresistant bacteria, which can increase the risk of outbreaks. Methods Samples were collected from the nasopharynx, hands, and lab coats of healthcare workers. The phenotypic identification was carried out using a VITEK®2 rapid test system. PCR tests for the mecA gene and the sequencing of the amplicons were performed. Staphylococcus epidermidis and Staphylococcus aureus phylogenies were reconstructed using the Bayesian inference. Results A total of 225 healthcare workers participated in this study. Of these, 21.3% were male and 78.7% female. S. epidermidis and S.aureus showed high levels of resistance to penicillin, ampicillin, erythromycin, tetracycline and cefoxitin. The prevalence of methicillin resistant S. aureus was 3.16% and methicillin resistant S. epidermidis was 100%. Multilocus sequence typing identified 23 new S. epidermidis sequence types, and one new allele and sequence type for S. aureus. The frequency of methicillin-resistant S. epidermidis in nursing and hemotherapy technicians as a percentage of the total number of healthcare workers was 5.8–3.1%, while the frequency of methicillin resistant S. aureus in hemotherapy technicians and biomedics, as a percentage of the total number of healthcare workers was 4.2–8.9%%. Conclusions The healthcare workers at the city’s blood bank, even when taking the necessary care with their hands, body and clothes, harbour methicillin-resistant S. aureus and S. epidermidis sequence types, which, as a potential source of multidrug resistant bacteria, can contribute to nosocomial infections among hematological patients.Cristina Motta FerreiraRoberto Alexandre Alves Barbosa FilhoGuilherme Motta Antunes FerreiraMarcus Vinicius Guimarães de LacerdaCintia Mara Costa de OliveiraVanderson de Souza SampaioLucyane Mendes SilvaAndreza Gomes PascoalWilliam Antunes FerreiraBMCarticleS. epidermidisMultirresistanceOxacillinSTCloneMicrobiologyQR1-502ENBMC Microbiology, Vol 21, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	S. epidermidis Multirresistance Oxacillin ST Clone Microbiology QR1-502
spellingShingle	S. epidermidis Multirresistance Oxacillin ST Clone Microbiology QR1-502 Cristina Motta Ferreira Roberto Alexandre Alves Barbosa Filho Guilherme Motta Antunes Ferreira Marcus Vinicius Guimarães de Lacerda Cintia Mara Costa de Oliveira Vanderson de Souza Sampaio Lucyane Mendes Silva Andreza Gomes Pascoal William Antunes Ferreira Molecular epidemiology of methicillin resistant Staphylococcus species in healthcare workers of a blood bank in the Brazilian Amazon
description	Abstract Background Healthcare workers are susceptible to colonization by multiresistant bacteria, which can increase the risk of outbreaks. Methods Samples were collected from the nasopharynx, hands, and lab coats of healthcare workers. The phenotypic identification was carried out using a VITEK®2 rapid test system. PCR tests for the mecA gene and the sequencing of the amplicons were performed. Staphylococcus epidermidis and Staphylococcus aureus phylogenies were reconstructed using the Bayesian inference. Results A total of 225 healthcare workers participated in this study. Of these, 21.3% were male and 78.7% female. S. epidermidis and S.aureus showed high levels of resistance to penicillin, ampicillin, erythromycin, tetracycline and cefoxitin. The prevalence of methicillin resistant S. aureus was 3.16% and methicillin resistant S. epidermidis was 100%. Multilocus sequence typing identified 23 new S. epidermidis sequence types, and one new allele and sequence type for S. aureus. The frequency of methicillin-resistant S. epidermidis in nursing and hemotherapy technicians as a percentage of the total number of healthcare workers was 5.8–3.1%, while the frequency of methicillin resistant S. aureus in hemotherapy technicians and biomedics, as a percentage of the total number of healthcare workers was 4.2–8.9%%. Conclusions The healthcare workers at the city’s blood bank, even when taking the necessary care with their hands, body and clothes, harbour methicillin-resistant S. aureus and S. epidermidis sequence types, which, as a potential source of multidrug resistant bacteria, can contribute to nosocomial infections among hematological patients.
format	article
author	Cristina Motta Ferreira Roberto Alexandre Alves Barbosa Filho Guilherme Motta Antunes Ferreira Marcus Vinicius Guimarães de Lacerda Cintia Mara Costa de Oliveira Vanderson de Souza Sampaio Lucyane Mendes Silva Andreza Gomes Pascoal William Antunes Ferreira
author_facet	Cristina Motta Ferreira Roberto Alexandre Alves Barbosa Filho Guilherme Motta Antunes Ferreira Marcus Vinicius Guimarães de Lacerda Cintia Mara Costa de Oliveira Vanderson de Souza Sampaio Lucyane Mendes Silva Andreza Gomes Pascoal William Antunes Ferreira
author_sort	Cristina Motta Ferreira
title	Molecular epidemiology of methicillin resistant Staphylococcus species in healthcare workers of a blood bank in the Brazilian Amazon
title_short	Molecular epidemiology of methicillin resistant Staphylococcus species in healthcare workers of a blood bank in the Brazilian Amazon
title_full	Molecular epidemiology of methicillin resistant Staphylococcus species in healthcare workers of a blood bank in the Brazilian Amazon
title_fullStr	Molecular epidemiology of methicillin resistant Staphylococcus species in healthcare workers of a blood bank in the Brazilian Amazon
title_full_unstemmed	Molecular epidemiology of methicillin resistant Staphylococcus species in healthcare workers of a blood bank in the Brazilian Amazon
title_sort	molecular epidemiology of methicillin resistant staphylococcus species in healthcare workers of a blood bank in the brazilian amazon
publisher	BMC
publishDate	2021
url	https://doaj.org/article/98368a6af62a446fb88c71c311ff49cb
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Molecular epidemiology of methicillin resistant Staphylococcus species in healthcare workers of a blood bank in the Brazilian Amazon

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