Differences between the cell populations from the peritenon and the tendon core with regard to their potential implication in tendon repair.

The role of intrinsic and extrinsic healing in injured tendons is still debated. In this study, we characterized cell plasticity, proliferative capacity, and migration characteristics as proxy measures of healing potential in cells derived from the peritenon (extrinsic healing) and compared these to...

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Autores principales:	Jennifer A Cadby, Evelyne Buehler, Charles Godbout, P René van Weeren, Jess G Snedeker
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/983b8807e2b24182818af69b786f1bee
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spelling	oai:doaj.org-article:983b8807e2b24182818af69b786f1bee2021-11-18T08:27:06ZDifferences between the cell populations from the peritenon and the tendon core with regard to their potential implication in tendon repair.1932-620310.1371/journal.pone.0092474https://doaj.org/article/983b8807e2b24182818af69b786f1bee2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24651449/?tool=EBIhttps://doaj.org/toc/1932-6203The role of intrinsic and extrinsic healing in injured tendons is still debated. In this study, we characterized cell plasticity, proliferative capacity, and migration characteristics as proxy measures of healing potential in cells derived from the peritenon (extrinsic healing) and compared these to cells from the tendon core (intrinsic healing). Both cell populations were extracted from horse superficial digital flexor tendon and characterized for tenogenic and matrix remodeling markers as well as for rates of migration and replication. Furthermore, colony-forming unit assays, multipotency assays, and real-time quantitative polymerase chain reaction analyses of markers of osteogenic and adipogenic differentiation after culture in induction media were performed. Finally, cellular capacity for differentiation towards a myofibroblastic phenotype was assessed. Our results demonstrate that both tendon- and peritenon-derived cell populations are capable of adipogenic and osteogenic differentiation, with higher expression of progenitor cell markers in peritenon cells. Cells from the peritenon also migrated faster, replicate more quickly, and show higher differentiation potential toward a myofibroblastic phenotype when compared to cells from the tendon core. Based on these data, we suggest that cells from the peritenon have substantial potential to influence tendon-healing outcome, warranting further scrutiny of their role.Jennifer A CadbyEvelyne BuehlerCharles GodboutP René van WeerenJess G SnedekerPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 3, p e92474 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jennifer A Cadby Evelyne Buehler Charles Godbout P René van Weeren Jess G Snedeker Differences between the cell populations from the peritenon and the tendon core with regard to their potential implication in tendon repair.
description	The role of intrinsic and extrinsic healing in injured tendons is still debated. In this study, we characterized cell plasticity, proliferative capacity, and migration characteristics as proxy measures of healing potential in cells derived from the peritenon (extrinsic healing) and compared these to cells from the tendon core (intrinsic healing). Both cell populations were extracted from horse superficial digital flexor tendon and characterized for tenogenic and matrix remodeling markers as well as for rates of migration and replication. Furthermore, colony-forming unit assays, multipotency assays, and real-time quantitative polymerase chain reaction analyses of markers of osteogenic and adipogenic differentiation after culture in induction media were performed. Finally, cellular capacity for differentiation towards a myofibroblastic phenotype was assessed. Our results demonstrate that both tendon- and peritenon-derived cell populations are capable of adipogenic and osteogenic differentiation, with higher expression of progenitor cell markers in peritenon cells. Cells from the peritenon also migrated faster, replicate more quickly, and show higher differentiation potential toward a myofibroblastic phenotype when compared to cells from the tendon core. Based on these data, we suggest that cells from the peritenon have substantial potential to influence tendon-healing outcome, warranting further scrutiny of their role.
format	article
author	Jennifer A Cadby Evelyne Buehler Charles Godbout P René van Weeren Jess G Snedeker
author_facet	Jennifer A Cadby Evelyne Buehler Charles Godbout P René van Weeren Jess G Snedeker
author_sort	Jennifer A Cadby
title	Differences between the cell populations from the peritenon and the tendon core with regard to their potential implication in tendon repair.
title_short	Differences between the cell populations from the peritenon and the tendon core with regard to their potential implication in tendon repair.
title_full	Differences between the cell populations from the peritenon and the tendon core with regard to their potential implication in tendon repair.
title_fullStr	Differences between the cell populations from the peritenon and the tendon core with regard to their potential implication in tendon repair.
title_full_unstemmed	Differences between the cell populations from the peritenon and the tendon core with regard to their potential implication in tendon repair.
title_sort	differences between the cell populations from the peritenon and the tendon core with regard to their potential implication in tendon repair.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/983b8807e2b24182818af69b786f1bee
work_keys_str_mv	AT jenniferacadby differencesbetweenthecellpopulationsfromtheperitenonandthetendoncorewithregardtotheirpotentialimplicationintendonrepair AT evelynebuehler differencesbetweenthecellpopulationsfromtheperitenonandthetendoncorewithregardtotheirpotentialimplicationintendonrepair AT charlesgodbout differencesbetweenthecellpopulationsfromtheperitenonandthetendoncorewithregardtotheirpotentialimplicationintendonrepair AT prenevanweeren differencesbetweenthecellpopulationsfromtheperitenonandthetendoncorewithregardtotheirpotentialimplicationintendonrepair AT jessgsnedeker differencesbetweenthecellpopulationsfromtheperitenonandthetendoncorewithregardtotheirpotentialimplicationintendonrepair
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Differences between the cell populations from the peritenon and the tendon core with regard to their potential implication in tendon repair.

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