Distribution and genome structures of temperate phages in acetic acid bacteria

Abstract Acetic acid bacteria (AAB) are industrial microorganisms used for vinegar fermentation. Herein, we investigated the distribution and genome structures of mitomycin C-inducible temperate phages in AAB. Transmission electron microscopy analysis revealed phage-like particles in 15 out of a tot...

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Autores principales:	Koki Omata, Naruhiro Hibi, Shigeru Nakano, Shuji Komoto, Kazuki Sato, Kei Nunokawa, Shoichi Amano, Kenji Ueda, Hideaki Takano
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/983cd71df7254a8f8e93078c3b208c43
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spelling	oai:doaj.org-article:983cd71df7254a8f8e93078c3b208c432021-11-08T10:48:11ZDistribution and genome structures of temperate phages in acetic acid bacteria10.1038/s41598-021-00998-w2045-2322https://doaj.org/article/983cd71df7254a8f8e93078c3b208c432021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-00998-whttps://doaj.org/toc/2045-2322Abstract Acetic acid bacteria (AAB) are industrial microorganisms used for vinegar fermentation. Herein, we investigated the distribution and genome structures of mitomycin C-inducible temperate phages in AAB. Transmission electron microscopy analysis revealed phage-like particles in 15 out of a total 177 acetic acid bacterial strains, all of which showed morphology similar to myoviridae-type phage. The complete genome sequences of the six phages derived from three strains each of Acetobacter and Komagataeibacter strains were determined, harboring a genome size ranging from 34,100 to 53,798 bp. A phage AP1 from A. pasteurianus NBRC 109446 was predicted as an active phage based on the genomic information, and actually had the ability to infect its phiAP1-cured strain. The attachment sites for phiAP1 were located in the 3’-end region of the tRNA ser gene. We also developed a chromosome-integrative vector, p2096int, based on the integrase function of phiAP1, and it was successfully integrated into the attachment site of the phiAP1-cured strain, which may be used as a valuable tool for the genetic engineering. Overall, this study showed the distribution of mitomycin C-inducible temperate phages in AAB, and identified the active temperate phage o f A. pasteurianus.Koki OmataNaruhiro HibiShigeru NakanoShuji KomotoKazuki SatoKei NunokawaShoichi AmanoKenji UedaHideaki TakanoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Koki Omata Naruhiro Hibi Shigeru Nakano Shuji Komoto Kazuki Sato Kei Nunokawa Shoichi Amano Kenji Ueda Hideaki Takano Distribution and genome structures of temperate phages in acetic acid bacteria
description	Abstract Acetic acid bacteria (AAB) are industrial microorganisms used for vinegar fermentation. Herein, we investigated the distribution and genome structures of mitomycin C-inducible temperate phages in AAB. Transmission electron microscopy analysis revealed phage-like particles in 15 out of a total 177 acetic acid bacterial strains, all of which showed morphology similar to myoviridae-type phage. The complete genome sequences of the six phages derived from three strains each of Acetobacter and Komagataeibacter strains were determined, harboring a genome size ranging from 34,100 to 53,798 bp. A phage AP1 from A. pasteurianus NBRC 109446 was predicted as an active phage based on the genomic information, and actually had the ability to infect its phiAP1-cured strain. The attachment sites for phiAP1 were located in the 3’-end region of the tRNA ser gene. We also developed a chromosome-integrative vector, p2096int, based on the integrase function of phiAP1, and it was successfully integrated into the attachment site of the phiAP1-cured strain, which may be used as a valuable tool for the genetic engineering. Overall, this study showed the distribution of mitomycin C-inducible temperate phages in AAB, and identified the active temperate phage o f A. pasteurianus.
format	article
author	Koki Omata Naruhiro Hibi Shigeru Nakano Shuji Komoto Kazuki Sato Kei Nunokawa Shoichi Amano Kenji Ueda Hideaki Takano
author_facet	Koki Omata Naruhiro Hibi Shigeru Nakano Shuji Komoto Kazuki Sato Kei Nunokawa Shoichi Amano Kenji Ueda Hideaki Takano
author_sort	Koki Omata
title	Distribution and genome structures of temperate phages in acetic acid bacteria
title_short	Distribution and genome structures of temperate phages in acetic acid bacteria
title_full	Distribution and genome structures of temperate phages in acetic acid bacteria
title_fullStr	Distribution and genome structures of temperate phages in acetic acid bacteria
title_full_unstemmed	Distribution and genome structures of temperate phages in acetic acid bacteria
title_sort	distribution and genome structures of temperate phages in acetic acid bacteria
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/983cd71df7254a8f8e93078c3b208c43
work_keys_str_mv	AT kokiomata distributionandgenomestructuresoftemperatephagesinaceticacidbacteria AT naruhirohibi distributionandgenomestructuresoftemperatephagesinaceticacidbacteria AT shigerunakano distributionandgenomestructuresoftemperatephagesinaceticacidbacteria AT shujikomoto distributionandgenomestructuresoftemperatephagesinaceticacidbacteria AT kazukisato distributionandgenomestructuresoftemperatephagesinaceticacidbacteria AT keinunokawa distributionandgenomestructuresoftemperatephagesinaceticacidbacteria AT shoichiamano distributionandgenomestructuresoftemperatephagesinaceticacidbacteria AT kenjiueda distributionandgenomestructuresoftemperatephagesinaceticacidbacteria AT hideakitakano distributionandgenomestructuresoftemperatephagesinaceticacidbacteria
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Distribution and genome structures of temperate phages in acetic acid bacteria

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