An innovative modification of the nutrient medium formulation for the isolation and differentiation of enterobacteria

In laboratory conditions, the modified nutrient differential diagnostic medium Drigalski agar with lactose is recommended to be used for cultivation (isolation) and differentiation of enterobacteria of the Enterobacteriaceae family. The differentiation of enterobacteria on a modified medium is carri...

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Autores principales:	Ermakov Vladimir, Titov Nikolay
Formato:	article
Lenguaje:	EN FR
Publicado:	EDP Sciences 2021
Materias:	Microbiology QR1-502 Physiology QP1-981 Zoology QL1-991
Acceso en línea:	https://doaj.org/article/9842af661eac4a95ae12a6c4265e8888
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spelling	oai:doaj.org-article:9842af661eac4a95ae12a6c4265e88882021-11-08T15:17:24ZAn innovative modification of the nutrient medium formulation for the isolation and differentiation of enterobacteria2117-445810.1051/bioconf/20213700063https://doaj.org/article/9842af661eac4a95ae12a6c4265e88882021-01-01T00:00:00Zhttps://www.bio-conferences.org/articles/bioconf/pdf/2021/09/bioconf_fies2021_00063.pdfhttps://doaj.org/toc/2117-4458In laboratory conditions, the modified nutrient differential diagnostic medium Drigalski agar with lactose is recommended to be used for cultivation (isolation) and differentiation of enterobacteria of the Enterobacteriaceae family. The differentiation of enterobacteria on a modified medium is carried out according to their ability to ferment lactose, mannitol, glucose, sucrose, gelatin and form hydrogen sulfide. The environment can also be used for conducting sanitary and microbiological studies of environmental objects. The environment can be used to perform the ONPG test. In the course of preparing the modified Drigalski agar for operation in laboratory conditions, we recommend using the medium according to one of the options: when one of the carbohydrates is added, the medium is poured into single-section reusable or disposable petri dishes; when two carbohydrates are added, the medium is poured into two-section reusable or disposable petri dishes; when a complex of carbohydrates lactose + mannitol and glucose + sucrose is added, the medium is poured into two-section reusable or disposable petri dishes; when using all four drives separately, the medium is dispensed into single-compartment reusable or disposable petri dishes. We recommend preparing modified Drigalski agar at a plant for the production of ready-made culture media with filling the medium in two-section petri dishes (complex of carbohydrates lactose + mannitol in one section, glucose + sucrose in another section), or with filling the medium in four-section petri dishes (with each carbohydrate in separate section).Ermakov VladimirTitov NikolayEDP SciencesarticleMicrobiologyQR1-502PhysiologyQP1-981ZoologyQL1-991ENFRBIO Web of Conferences, Vol 37, p 00063 (2021)
institution	DOAJ
collection	DOAJ
language	EN FR
topic	Microbiology QR1-502 Physiology QP1-981 Zoology QL1-991
spellingShingle	Microbiology QR1-502 Physiology QP1-981 Zoology QL1-991 Ermakov Vladimir Titov Nikolay An innovative modification of the nutrient medium formulation for the isolation and differentiation of enterobacteria
description	In laboratory conditions, the modified nutrient differential diagnostic medium Drigalski agar with lactose is recommended to be used for cultivation (isolation) and differentiation of enterobacteria of the Enterobacteriaceae family. The differentiation of enterobacteria on a modified medium is carried out according to their ability to ferment lactose, mannitol, glucose, sucrose, gelatin and form hydrogen sulfide. The environment can also be used for conducting sanitary and microbiological studies of environmental objects. The environment can be used to perform the ONPG test. In the course of preparing the modified Drigalski agar for operation in laboratory conditions, we recommend using the medium according to one of the options: when one of the carbohydrates is added, the medium is poured into single-section reusable or disposable petri dishes; when two carbohydrates are added, the medium is poured into two-section reusable or disposable petri dishes; when a complex of carbohydrates lactose + mannitol and glucose + sucrose is added, the medium is poured into two-section reusable or disposable petri dishes; when using all four drives separately, the medium is dispensed into single-compartment reusable or disposable petri dishes. We recommend preparing modified Drigalski agar at a plant for the production of ready-made culture media with filling the medium in two-section petri dishes (complex of carbohydrates lactose + mannitol in one section, glucose + sucrose in another section), or with filling the medium in four-section petri dishes (with each carbohydrate in separate section).
format	article
author	Ermakov Vladimir Titov Nikolay
author_facet	Ermakov Vladimir Titov Nikolay
author_sort	Ermakov Vladimir
title	An innovative modification of the nutrient medium formulation for the isolation and differentiation of enterobacteria
title_short	An innovative modification of the nutrient medium formulation for the isolation and differentiation of enterobacteria
title_full	An innovative modification of the nutrient medium formulation for the isolation and differentiation of enterobacteria
title_fullStr	An innovative modification of the nutrient medium formulation for the isolation and differentiation of enterobacteria
title_full_unstemmed	An innovative modification of the nutrient medium formulation for the isolation and differentiation of enterobacteria
title_sort	innovative modification of the nutrient medium formulation for the isolation and differentiation of enterobacteria
publisher	EDP Sciences
publishDate	2021
url	https://doaj.org/article/9842af661eac4a95ae12a6c4265e8888
work_keys_str_mv	AT ermakovvladimir aninnovativemodificationofthenutrientmediumformulationfortheisolationanddifferentiationofenterobacteria AT titovnikolay aninnovativemodificationofthenutrientmediumformulationfortheisolationanddifferentiationofenterobacteria AT ermakovvladimir innovativemodificationofthenutrientmediumformulationfortheisolationanddifferentiationofenterobacteria AT titovnikolay innovativemodificationofthenutrientmediumformulationfortheisolationanddifferentiationofenterobacteria
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An innovative modification of the nutrient medium formulation for the isolation and differentiation of enterobacteria

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