Beam image-shift accelerated data acquisition for near-atomic resolution single-particle cryo-electron tomography

Tomographic reconstructions of cryopreserved specimens enable in-situ structural studies. Here, the authors present the beam image-shift electron cryo-tomography (BISECT) approach that accelerates data collection speed and improves the map resolution compared to earlier approaches and present the in...

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Autores principales:	Jonathan Bouvette, Hsuan-Fu Liu, Xiaochen Du, Ye Zhou, Andrew P. Sikkema, Juliana da Fonseca Rezende e Mello, Bradley P. Klemm, Rick Huang, Roel M. Schaaper, Mario J. Borgnia, Alberto Bartesaghi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/984e7bb3da884354b7ff3bc7cde40146
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spelling	oai:doaj.org-article:984e7bb3da884354b7ff3bc7cde401462021-12-02T14:25:25ZBeam image-shift accelerated data acquisition for near-atomic resolution single-particle cryo-electron tomography10.1038/s41467-021-22251-82041-1723https://doaj.org/article/984e7bb3da884354b7ff3bc7cde401462021-03-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-22251-8https://doaj.org/toc/2041-1723Tomographic reconstructions of cryopreserved specimens enable in-situ structural studies. Here, the authors present the beam image-shift electron cryo-tomography (BISECT) approach that accelerates data collection speed and improves the map resolution compared to earlier approaches and present the in vitro structure of a 300 kDa protein complex that was solved at 3.6 Å resolution as a test case.Jonathan BouvetteHsuan-Fu LiuXiaochen DuYe ZhouAndrew P. SikkemaJuliana da Fonseca Rezende e MelloBradley P. KlemmRick HuangRoel M. SchaaperMario J. BorgniaAlberto BartesaghiNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Jonathan Bouvette Hsuan-Fu Liu Xiaochen Du Ye Zhou Andrew P. Sikkema Juliana da Fonseca Rezende e Mello Bradley P. Klemm Rick Huang Roel M. Schaaper Mario J. Borgnia Alberto Bartesaghi Beam image-shift accelerated data acquisition for near-atomic resolution single-particle cryo-electron tomography
description	Tomographic reconstructions of cryopreserved specimens enable in-situ structural studies. Here, the authors present the beam image-shift electron cryo-tomography (BISECT) approach that accelerates data collection speed and improves the map resolution compared to earlier approaches and present the in vitro structure of a 300 kDa protein complex that was solved at 3.6 Å resolution as a test case.
format	article
author	Jonathan Bouvette Hsuan-Fu Liu Xiaochen Du Ye Zhou Andrew P. Sikkema Juliana da Fonseca Rezende e Mello Bradley P. Klemm Rick Huang Roel M. Schaaper Mario J. Borgnia Alberto Bartesaghi
author_facet	Jonathan Bouvette Hsuan-Fu Liu Xiaochen Du Ye Zhou Andrew P. Sikkema Juliana da Fonseca Rezende e Mello Bradley P. Klemm Rick Huang Roel M. Schaaper Mario J. Borgnia Alberto Bartesaghi
author_sort	Jonathan Bouvette
title	Beam image-shift accelerated data acquisition for near-atomic resolution single-particle cryo-electron tomography
title_short	Beam image-shift accelerated data acquisition for near-atomic resolution single-particle cryo-electron tomography
title_full	Beam image-shift accelerated data acquisition for near-atomic resolution single-particle cryo-electron tomography
title_fullStr	Beam image-shift accelerated data acquisition for near-atomic resolution single-particle cryo-electron tomography
title_full_unstemmed	Beam image-shift accelerated data acquisition for near-atomic resolution single-particle cryo-electron tomography
title_sort	beam image-shift accelerated data acquisition for near-atomic resolution single-particle cryo-electron tomography
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/984e7bb3da884354b7ff3bc7cde40146
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Beam image-shift accelerated data acquisition for near-atomic resolution single-particle cryo-electron tomography

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