A novel ion-exchange carrier based upon liposome-encapsulated montmorillonite for ophthalmic delivery of betaxolol hydrochloride

Yi Huang,1 Qi Tao,2 Dongzhi Hou,1 Sheng Hu,1 Shuangyan Tian,1 Yanzhong Chen,3 Ruyi Gui,1 Lingling Yang,4 Yao Wang5 1College of Pharmacy, Guangdong Pharmaceutical University, 2Key Laboratory of Mineralogy and Metallogeny, Chinese Academy of Sciences, Guangdong Provincial Key Laboratory of Mineral Ph...

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Autores principales:	Huang Y, Tao Q, Hou DZ, Hu S, Tian SY, Chen YZ, Gui RY, Yang LL, Wang Y
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	liposome montmorillonite irritation precorneal retention time intraocular optical pressure Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/984eade2361a4ed4b91ae21f13deba83
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spelling	oai:doaj.org-article:984eade2361a4ed4b91ae21f13deba832021-12-02T00:46:37ZA novel ion-exchange carrier based upon liposome-encapsulated montmorillonite for ophthalmic delivery of betaxolol hydrochloride1178-2013https://doaj.org/article/984eade2361a4ed4b91ae21f13deba832017-03-01T00:00:00Zhttps://www.dovepress.com/a-novel-ion-exchange-carrier-based-upon-liposome-encapsulated--montmor-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Yi Huang,1 Qi Tao,2 Dongzhi Hou,1 Sheng Hu,1 Shuangyan Tian,1 Yanzhong Chen,3 Ruyi Gui,1 Lingling Yang,4 Yao Wang5 1College of Pharmacy, Guangdong Pharmaceutical University, 2Key Laboratory of Mineralogy and Metallogeny, Chinese Academy of Sciences, Guangdong Provincial Key Laboratory of Mineral Physics and Materials, 3Guangdong Provincial Key Laboratory of Advanced Drug Delivery Systems, Guangdong Pharmaceutical University, Guangzhou, 4State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Shandong Eye Institute, Shandong Academy of Medical Sciences, 5Qingdao Eye Hospital, Shandong Eye Institute, Shandong Academy of Medical Sciences, Qingdao, China Abstract: As a novel ion-exchange carrier with high surface area and excellent exchangeability, montmorillonite (Mt) was intercalated with betaxolol hydrochloride (BH) to form a nanocomposite and then encapsulated by liposomes (Mt-BH-LPs) for an ophthalmic drug-delivery system. The Mt-BH and Mt-BH-LPs were prepared by an acidification process and ethanol injection combined with ammonium sulfate gradient methods. The successful formation of Mt-BH and Mt-BH-LPs was verified by thermogravimetric analysis, X-ray diffraction, Fourier-transform infrared spectra, and transmission electron microscopy. Mt-BH-LPs possessed the favorable physical characteristics of encapsulation efficiency, drug loading, mean particle size, and ζ-potential. In vitro release studies indicated Mt-BH-LPs effectively maintained a relatively sustained slow release. Immortalized human corneal epithelial cell cytotoxicity, in vivo rabbit eye-irritation tests, and chorioallantoic membrane–trypan blue staining all revealed that Mt-BH-LPs had no obvious irritation on ocular tissues. A new in vitro tear-turnover model, including inserts containing human corneal epithelial cells, was designed to evaluate the precorneal retention time of Mt-BH-LPs. The results showed that Mt-BH-LPs maintained a certain BH concentration in tear fluid for a longer period than the BH solution. In vivo precorneal retention studies also indicated Mt-BH-LPs prolonged drug retention on the ocular surface more than the BH solution. Furthermore, pharmacodynamic studies showed that Mt-BH-LPs had a prolonged effect on decreasing intraocular optical pressure in rabbits. Our results demonstrated that Mt-BH-LPs have potential as an ophthalmic delivery system. Keywords: liposome, montmorillonite, irritation, precorneal retention time, intraocular optical pressureHuang YTao QHou DZHu STian SYChen YZGui RYYang LLWang YDove Medical Pressarticleliposomemontmorilloniteirritationprecorneal retention timeintraocular optical pressureMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 1731-1745 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	liposome montmorillonite irritation precorneal retention time intraocular optical pressure Medicine (General) R5-920
spellingShingle	liposome montmorillonite irritation precorneal retention time intraocular optical pressure Medicine (General) R5-920 Huang Y Tao Q Hou DZ Hu S Tian SY Chen YZ Gui RY Yang LL Wang Y A novel ion-exchange carrier based upon liposome-encapsulated montmorillonite for ophthalmic delivery of betaxolol hydrochloride
description	Yi Huang,1 Qi Tao,2 Dongzhi Hou,1 Sheng Hu,1 Shuangyan Tian,1 Yanzhong Chen,3 Ruyi Gui,1 Lingling Yang,4 Yao Wang5 1College of Pharmacy, Guangdong Pharmaceutical University, 2Key Laboratory of Mineralogy and Metallogeny, Chinese Academy of Sciences, Guangdong Provincial Key Laboratory of Mineral Physics and Materials, 3Guangdong Provincial Key Laboratory of Advanced Drug Delivery Systems, Guangdong Pharmaceutical University, Guangzhou, 4State Key Laboratory Cultivation Base, Shandong Provincial Key Laboratory of Ophthalmology, Shandong Eye Institute, Shandong Academy of Medical Sciences, 5Qingdao Eye Hospital, Shandong Eye Institute, Shandong Academy of Medical Sciences, Qingdao, China Abstract: As a novel ion-exchange carrier with high surface area and excellent exchangeability, montmorillonite (Mt) was intercalated with betaxolol hydrochloride (BH) to form a nanocomposite and then encapsulated by liposomes (Mt-BH-LPs) for an ophthalmic drug-delivery system. The Mt-BH and Mt-BH-LPs were prepared by an acidification process and ethanol injection combined with ammonium sulfate gradient methods. The successful formation of Mt-BH and Mt-BH-LPs was verified by thermogravimetric analysis, X-ray diffraction, Fourier-transform infrared spectra, and transmission electron microscopy. Mt-BH-LPs possessed the favorable physical characteristics of encapsulation efficiency, drug loading, mean particle size, and ζ-potential. In vitro release studies indicated Mt-BH-LPs effectively maintained a relatively sustained slow release. Immortalized human corneal epithelial cell cytotoxicity, in vivo rabbit eye-irritation tests, and chorioallantoic membrane–trypan blue staining all revealed that Mt-BH-LPs had no obvious irritation on ocular tissues. A new in vitro tear-turnover model, including inserts containing human corneal epithelial cells, was designed to evaluate the precorneal retention time of Mt-BH-LPs. The results showed that Mt-BH-LPs maintained a certain BH concentration in tear fluid for a longer period than the BH solution. In vivo precorneal retention studies also indicated Mt-BH-LPs prolonged drug retention on the ocular surface more than the BH solution. Furthermore, pharmacodynamic studies showed that Mt-BH-LPs had a prolonged effect on decreasing intraocular optical pressure in rabbits. Our results demonstrated that Mt-BH-LPs have potential as an ophthalmic delivery system. Keywords: liposome, montmorillonite, irritation, precorneal retention time, intraocular optical pressure
format	article
author	Huang Y Tao Q Hou DZ Hu S Tian SY Chen YZ Gui RY Yang LL Wang Y
author_facet	Huang Y Tao Q Hou DZ Hu S Tian SY Chen YZ Gui RY Yang LL Wang Y
author_sort	Huang Y
title	A novel ion-exchange carrier based upon liposome-encapsulated montmorillonite for ophthalmic delivery of betaxolol hydrochloride
title_short	A novel ion-exchange carrier based upon liposome-encapsulated montmorillonite for ophthalmic delivery of betaxolol hydrochloride
title_full	A novel ion-exchange carrier based upon liposome-encapsulated montmorillonite for ophthalmic delivery of betaxolol hydrochloride
title_fullStr	A novel ion-exchange carrier based upon liposome-encapsulated montmorillonite for ophthalmic delivery of betaxolol hydrochloride
title_full_unstemmed	A novel ion-exchange carrier based upon liposome-encapsulated montmorillonite for ophthalmic delivery of betaxolol hydrochloride
title_sort	novel ion-exchange carrier based upon liposome-encapsulated montmorillonite for ophthalmic delivery of betaxolol hydrochloride
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/984eade2361a4ed4b91ae21f13deba83
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A novel ion-exchange carrier based upon liposome-encapsulated montmorillonite for ophthalmic delivery of betaxolol hydrochloride

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