Generation, purification and transplantation of photoreceptors derived from human induced pluripotent stem cells.

<h4>Background</h4>Inherited and acquired retinal degenerations are frequent causes of visual impairment and photoreceptor cell replacement therapy may restore visual function to these individuals. To provide a source of new retinal neurons for cell based therapies, we developed methods...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Deepak A Lamba, Andrew McUsic, Roli K Hirata, Pei-Rong Wang, David Russell, Thomas A Reh
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/985078a234eb4cbcaeac81201ba8f71b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:985078a234eb4cbcaeac81201ba8f71b
record_format	dspace
spelling	oai:doaj.org-article:985078a234eb4cbcaeac81201ba8f71b2021-11-25T06:26:34ZGeneration, purification and transplantation of photoreceptors derived from human induced pluripotent stem cells.1932-620310.1371/journal.pone.0008763https://doaj.org/article/985078a234eb4cbcaeac81201ba8f71b2010-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20098701/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Inherited and acquired retinal degenerations are frequent causes of visual impairment and photoreceptor cell replacement therapy may restore visual function to these individuals. To provide a source of new retinal neurons for cell based therapies, we developed methods to derive retinal progenitors from human ES cells.<h4>Methodology/physical findings</h4>In this report we have used a similar method to direct induced pluripotent stem cells (iPS) from human fibroblasts to a retinal progenitor fate, competent to generate photoreceptors. We also found we could purify the photoreceptors derived from the iPS cells using fluorescence activated cell sorting (FACS) after labeling photoreceptors with a lentivirus driving GFP from the IRBP cis-regulatory sequences. Moreover, we found that when we transplanted the FACS purified iPSC derived photoreceptors, they were able to integrate into a normal mouse retina and express photoreceptor markers.<h4>Conclusions</h4>This report provides evidence that enriched populations of human photoreceptors can be derived from iPS cells.Deepak A LambaAndrew McUsicRoli K HirataPei-Rong WangDavid RussellThomas A RehPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 1, p e8763 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Deepak A Lamba Andrew McUsic Roli K Hirata Pei-Rong Wang David Russell Thomas A Reh Generation, purification and transplantation of photoreceptors derived from human induced pluripotent stem cells.
description	<h4>Background</h4>Inherited and acquired retinal degenerations are frequent causes of visual impairment and photoreceptor cell replacement therapy may restore visual function to these individuals. To provide a source of new retinal neurons for cell based therapies, we developed methods to derive retinal progenitors from human ES cells.<h4>Methodology/physical findings</h4>In this report we have used a similar method to direct induced pluripotent stem cells (iPS) from human fibroblasts to a retinal progenitor fate, competent to generate photoreceptors. We also found we could purify the photoreceptors derived from the iPS cells using fluorescence activated cell sorting (FACS) after labeling photoreceptors with a lentivirus driving GFP from the IRBP cis-regulatory sequences. Moreover, we found that when we transplanted the FACS purified iPSC derived photoreceptors, they were able to integrate into a normal mouse retina and express photoreceptor markers.<h4>Conclusions</h4>This report provides evidence that enriched populations of human photoreceptors can be derived from iPS cells.
format	article
author	Deepak A Lamba Andrew McUsic Roli K Hirata Pei-Rong Wang David Russell Thomas A Reh
author_facet	Deepak A Lamba Andrew McUsic Roli K Hirata Pei-Rong Wang David Russell Thomas A Reh
author_sort	Deepak A Lamba
title	Generation, purification and transplantation of photoreceptors derived from human induced pluripotent stem cells.
title_short	Generation, purification and transplantation of photoreceptors derived from human induced pluripotent stem cells.
title_full	Generation, purification and transplantation of photoreceptors derived from human induced pluripotent stem cells.
title_fullStr	Generation, purification and transplantation of photoreceptors derived from human induced pluripotent stem cells.
title_full_unstemmed	Generation, purification and transplantation of photoreceptors derived from human induced pluripotent stem cells.
title_sort	generation, purification and transplantation of photoreceptors derived from human induced pluripotent stem cells.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/985078a234eb4cbcaeac81201ba8f71b
work_keys_str_mv	AT deepakalamba generationpurificationandtransplantationofphotoreceptorsderivedfromhumaninducedpluripotentstemcells AT andrewmcusic generationpurificationandtransplantationofphotoreceptorsderivedfromhumaninducedpluripotentstemcells AT rolikhirata generationpurificationandtransplantationofphotoreceptorsderivedfromhumaninducedpluripotentstemcells AT peirongwang generationpurificationandtransplantationofphotoreceptorsderivedfromhumaninducedpluripotentstemcells AT davidrussell generationpurificationandtransplantationofphotoreceptorsderivedfromhumaninducedpluripotentstemcells AT thomasareh generationpurificationandtransplantationofphotoreceptorsderivedfromhumaninducedpluripotentstemcells
_version_	1718413758468980736

Generation, purification and transplantation of photoreceptors derived from human induced pluripotent stem cells.

Ejemplares similares