Improved functionality of Ligilactobacillus salivarius Li01 in alleviating colonic inflammation by layer-by-layer microencapsulation

Abstract The low viability during gastrointestinal transit and poor mucoadhesion considerably limits the effectiveness of Ligilactobacillus salivarius Li01 (Li01) in regulating gut microbiota and alleviating inflammatory bowel disease (IBD). In this study, a delivery system was designed through laye...

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Autores principales:	Mingfei Yao, Yanmeng Lu, Ting Zhang, Jiaojiao Xie, Shengyi Han, Shuobo Zhang, Yiqiu Fei, Zongxin Ling, Jingjing Wu, Yue Hu, Shouling Ji, Hao Chen, Björn Berglund, Lanjuan Li
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Microbial ecology QR100-130
Acceso en línea:	https://doaj.org/article/9855046786c34d518992166806c86a84
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spelling	oai:doaj.org-article:9855046786c34d518992166806c86a842021-12-02T16:24:50ZImproved functionality of Ligilactobacillus salivarius Li01 in alleviating colonic inflammation by layer-by-layer microencapsulation10.1038/s41522-021-00228-12055-5008https://doaj.org/article/9855046786c34d518992166806c86a842021-07-01T00:00:00Zhttps://doi.org/10.1038/s41522-021-00228-1https://doaj.org/toc/2055-5008Abstract The low viability during gastrointestinal transit and poor mucoadhesion considerably limits the effectiveness of Ligilactobacillus salivarius Li01 (Li01) in regulating gut microbiota and alleviating inflammatory bowel disease (IBD). In this study, a delivery system was designed through layer-by-layer (LbL) encapsulating a single Li01cell with chitosan and alginate. The layers were strengthened by cross-linking to form a firm and mucoadhesive shell (~10 nm thickness) covering the bacterial cell. The LbL Li01 displayed improved viability under simulated gastrointestinal conditions and mucoadhesive function. Almost no cells could be detected among the free Li01 after 2 h incubation in digestive fluids, while for LbL Li01, the total reduction was around 3 log CFU/mL and the viable number of cells remained above 6 log CFU/mL. Besides, a 5-fold increase in the value of rupture length and a two-fold increase in the number of peaks were found in the (bacteria-mucin) adhesion curves of LbL Li01, compared to those of free Li01. Oral administration with LbL Li01 on colitis mice facilitated intestinal barrier recovery and restoration of the gut microbiota. The improved functionality of Li01 by LbL encapsulation could increase the potential for the probiotic to be used in clinical applications to treat IBD; this should be explored in future studies.Mingfei YaoYanmeng LuTing ZhangJiaojiao XieShengyi HanShuobo ZhangYiqiu FeiZongxin LingJingjing WuYue HuShouling JiHao ChenBjörn BerglundLanjuan LiNature PortfolioarticleMicrobial ecologyQR100-130ENnpj Biofilms and Microbiomes, Vol 7, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbial ecology QR100-130
spellingShingle	Microbial ecology QR100-130 Mingfei Yao Yanmeng Lu Ting Zhang Jiaojiao Xie Shengyi Han Shuobo Zhang Yiqiu Fei Zongxin Ling Jingjing Wu Yue Hu Shouling Ji Hao Chen Björn Berglund Lanjuan Li Improved functionality of Ligilactobacillus salivarius Li01 in alleviating colonic inflammation by layer-by-layer microencapsulation
description	Abstract The low viability during gastrointestinal transit and poor mucoadhesion considerably limits the effectiveness of Ligilactobacillus salivarius Li01 (Li01) in regulating gut microbiota and alleviating inflammatory bowel disease (IBD). In this study, a delivery system was designed through layer-by-layer (LbL) encapsulating a single Li01cell with chitosan and alginate. The layers were strengthened by cross-linking to form a firm and mucoadhesive shell (~10 nm thickness) covering the bacterial cell. The LbL Li01 displayed improved viability under simulated gastrointestinal conditions and mucoadhesive function. Almost no cells could be detected among the free Li01 after 2 h incubation in digestive fluids, while for LbL Li01, the total reduction was around 3 log CFU/mL and the viable number of cells remained above 6 log CFU/mL. Besides, a 5-fold increase in the value of rupture length and a two-fold increase in the number of peaks were found in the (bacteria-mucin) adhesion curves of LbL Li01, compared to those of free Li01. Oral administration with LbL Li01 on colitis mice facilitated intestinal barrier recovery and restoration of the gut microbiota. The improved functionality of Li01 by LbL encapsulation could increase the potential for the probiotic to be used in clinical applications to treat IBD; this should be explored in future studies.
format	article
author	Mingfei Yao Yanmeng Lu Ting Zhang Jiaojiao Xie Shengyi Han Shuobo Zhang Yiqiu Fei Zongxin Ling Jingjing Wu Yue Hu Shouling Ji Hao Chen Björn Berglund Lanjuan Li
author_facet	Mingfei Yao Yanmeng Lu Ting Zhang Jiaojiao Xie Shengyi Han Shuobo Zhang Yiqiu Fei Zongxin Ling Jingjing Wu Yue Hu Shouling Ji Hao Chen Björn Berglund Lanjuan Li
author_sort	Mingfei Yao
title	Improved functionality of Ligilactobacillus salivarius Li01 in alleviating colonic inflammation by layer-by-layer microencapsulation
title_short	Improved functionality of Ligilactobacillus salivarius Li01 in alleviating colonic inflammation by layer-by-layer microencapsulation
title_full	Improved functionality of Ligilactobacillus salivarius Li01 in alleviating colonic inflammation by layer-by-layer microencapsulation
title_fullStr	Improved functionality of Ligilactobacillus salivarius Li01 in alleviating colonic inflammation by layer-by-layer microencapsulation
title_full_unstemmed	Improved functionality of Ligilactobacillus salivarius Li01 in alleviating colonic inflammation by layer-by-layer microencapsulation
title_sort	improved functionality of ligilactobacillus salivarius li01 in alleviating colonic inflammation by layer-by-layer microencapsulation
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9855046786c34d518992166806c86a84
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Improved functionality of Ligilactobacillus salivarius Li01 in alleviating colonic inflammation by layer-by-layer microencapsulation

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