Mining Middle Eastern and Central Asian Barley Germplasm to Understand Diversity for Resistance to <i>Puccinia hordei</i>, Causal Agent of Leaf Rust
Vast collections of barley germplasm have been established and conserved in various global gene banks. These collections hold tremendous genetic diversity for resistance genes to <i>Puccinia hordei</i>, a causal agent of barley leaf rust. This study was undertaken to discover, characteri...
Guardado en:
| Autores principales: | , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/985840e2cdb943b7bfaf69932904f1f3 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| Sumario: | Vast collections of barley germplasm have been established and conserved in various global gene banks. These collections hold tremendous genetic diversity for resistance genes to <i>Puccinia hordei</i>, a causal agent of barley leaf rust. This study was undertaken to discover, characterize and postulate the known <i>Rph</i> genes (resistance to <i>Puccinia hordei</i>) and identify novel sources of ASR (all-stage resistance) and APR (adult plant resistance) to <i>P. hordei</i>. A core set of 315 barley lines were rust-tested as seedlings for their response to eight Australian pathotypes of <i>P. hordei</i> and genotyped with molecular markers linked to the known characterised ASR and APR genes. These tests led to the postulation of ASR leaf rust resistance genes <i>Rph1</i>, <i>Rph2</i>, <i>Rph3, Rph9</i>.am, <i>Rph12</i>, <i>Rph15</i>, <i>Rph19</i> and <i>Rph25</i> singly or in combination. Field tests revealed that the vast majority of lines (84%) carried APR. Genotyping of the APR-carrying lines with markers bPb-0837, Ebmac0603 and sun43-44 identified lines that likely carry the known APR genes <i>Rph20</i>, <i>Rph23</i> and <i>Rph24</i> singly or in combination. Thirty-nine per cent of the lines were negative for all the three markers and were thus postulated to carry uncharacterized APR. The sources of resistance identified in this study provide a valuable resource to breeders for further utilization and diversifying the genetic basis of leaf rust resistance in barley. |
|---|