Mining Middle Eastern and Central Asian Barley Germplasm to Understand Diversity for Resistance to <i>Puccinia hordei</i>, Causal Agent of Leaf Rust

Vast collections of barley germplasm have been established and conserved in various global gene banks. These collections hold tremendous genetic diversity for resistance genes to <i>Puccinia hordei</i>, a causal agent of barley leaf rust. This study was undertaken to discover, characteri...

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Autores principales:	Mehnaz Mehnaz, Peter M. Dracatos, Robert F. Park, Davinder Singh
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	barley leaf rust <i>Puccinia hordei</i> resistance <i>Rph</i> gene postulation Agriculture S
Acceso en línea:	https://doaj.org/article/985840e2cdb943b7bfaf69932904f1f3
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spelling	oai:doaj.org-article:985840e2cdb943b7bfaf69932904f1f32021-11-25T16:04:14ZMining Middle Eastern and Central Asian Barley Germplasm to Understand Diversity for Resistance to <i>Puccinia hordei</i>, Causal Agent of Leaf Rust10.3390/agronomy111121462073-4395https://doaj.org/article/985840e2cdb943b7bfaf69932904f1f32021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4395/11/11/2146https://doaj.org/toc/2073-4395Vast collections of barley germplasm have been established and conserved in various global gene banks. These collections hold tremendous genetic diversity for resistance genes to <i>Puccinia hordei</i>, a causal agent of barley leaf rust. This study was undertaken to discover, characterize and postulate the known <i>Rph</i> genes (resistance to <i>Puccinia hordei</i>) and identify novel sources of ASR (all-stage resistance) and APR (adult plant resistance) to <i>P. hordei</i>. A core set of 315 barley lines were rust-tested as seedlings for their response to eight Australian pathotypes of <i>P. hordei</i> and genotyped with molecular markers linked to the known characterised ASR and APR genes. These tests led to the postulation of ASR leaf rust resistance genes <i>Rph1</i>, <i>Rph2</i>, <i>Rph3, Rph9</i>.am, <i>Rph12</i>, <i>Rph15</i>, <i>Rph19</i> and <i>Rph25</i> singly or in combination. Field tests revealed that the vast majority of lines (84%) carried APR. Genotyping of the APR-carrying lines with markers bPb-0837, Ebmac0603 and sun43-44 identified lines that likely carry the known APR genes <i>Rph20</i>, <i>Rph23</i> and <i>Rph24</i> singly or in combination. Thirty-nine per cent of the lines were negative for all the three markers and were thus postulated to carry uncharacterized APR. The sources of resistance identified in this study provide a valuable resource to breeders for further utilization and diversifying the genetic basis of leaf rust resistance in barley.Mehnaz MehnazPeter M. DracatosRobert F. ParkDavinder SinghMDPI AGarticlebarleyleaf rust<i>Puccinia hordei</i>resistance<i>Rph</i>gene postulationAgricultureSENAgronomy, Vol 11, Iss 2146, p 2146 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	barley leaf rust <i>Puccinia hordei</i> resistance <i>Rph</i> gene postulation Agriculture S
spellingShingle	barley leaf rust <i>Puccinia hordei</i> resistance <i>Rph</i> gene postulation Agriculture S Mehnaz Mehnaz Peter M. Dracatos Robert F. Park Davinder Singh Mining Middle Eastern and Central Asian Barley Germplasm to Understand Diversity for Resistance to <i>Puccinia hordei</i>, Causal Agent of Leaf Rust
description	Vast collections of barley germplasm have been established and conserved in various global gene banks. These collections hold tremendous genetic diversity for resistance genes to <i>Puccinia hordei</i>, a causal agent of barley leaf rust. This study was undertaken to discover, characterize and postulate the known <i>Rph</i> genes (resistance to <i>Puccinia hordei</i>) and identify novel sources of ASR (all-stage resistance) and APR (adult plant resistance) to <i>P. hordei</i>. A core set of 315 barley lines were rust-tested as seedlings for their response to eight Australian pathotypes of <i>P. hordei</i> and genotyped with molecular markers linked to the known characterised ASR and APR genes. These tests led to the postulation of ASR leaf rust resistance genes <i>Rph1</i>, <i>Rph2</i>, <i>Rph3, Rph9</i>.am, <i>Rph12</i>, <i>Rph15</i>, <i>Rph19</i> and <i>Rph25</i> singly or in combination. Field tests revealed that the vast majority of lines (84%) carried APR. Genotyping of the APR-carrying lines with markers bPb-0837, Ebmac0603 and sun43-44 identified lines that likely carry the known APR genes <i>Rph20</i>, <i>Rph23</i> and <i>Rph24</i> singly or in combination. Thirty-nine per cent of the lines were negative for all the three markers and were thus postulated to carry uncharacterized APR. The sources of resistance identified in this study provide a valuable resource to breeders for further utilization and diversifying the genetic basis of leaf rust resistance in barley.
format	article
author	Mehnaz Mehnaz Peter M. Dracatos Robert F. Park Davinder Singh
author_facet	Mehnaz Mehnaz Peter M. Dracatos Robert F. Park Davinder Singh
author_sort	Mehnaz Mehnaz
title	Mining Middle Eastern and Central Asian Barley Germplasm to Understand Diversity for Resistance to <i>Puccinia hordei</i>, Causal Agent of Leaf Rust
title_short	Mining Middle Eastern and Central Asian Barley Germplasm to Understand Diversity for Resistance to <i>Puccinia hordei</i>, Causal Agent of Leaf Rust
title_full	Mining Middle Eastern and Central Asian Barley Germplasm to Understand Diversity for Resistance to <i>Puccinia hordei</i>, Causal Agent of Leaf Rust
title_fullStr	Mining Middle Eastern and Central Asian Barley Germplasm to Understand Diversity for Resistance to <i>Puccinia hordei</i>, Causal Agent of Leaf Rust
title_full_unstemmed	Mining Middle Eastern and Central Asian Barley Germplasm to Understand Diversity for Resistance to <i>Puccinia hordei</i>, Causal Agent of Leaf Rust
title_sort	mining middle eastern and central asian barley germplasm to understand diversity for resistance to <i>puccinia hordei</i>, causal agent of leaf rust
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/985840e2cdb943b7bfaf69932904f1f3
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Mining Middle Eastern and Central Asian Barley Germplasm to Understand Diversity for Resistance to <i>Puccinia hordei</i>, Causal Agent of Leaf Rust

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