Molecular mechanisms of exopolysaccharide from Aphanothece halaphytica (EPSAH) induced apoptosis in HeLa cells.

The present study aims to investigate the pharmacological effect of the exopolysaccharides from Aphanothece halophytica GR02 (EPSAH) on the HeLa human cervical cancer cell line. HeLa cells were cultured in RPMI-1640-10% FBS medium containing with or without different concentrations of EPSAH. Cell vi...

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Autores principales:	Yu Ou, Shuya Xu, Dandan Zhu, Xuegan Yang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/985acff01a5044b1a83f8668bb44e808
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spelling	oai:doaj.org-article:985acff01a5044b1a83f8668bb44e8082021-11-18T08:35:58ZMolecular mechanisms of exopolysaccharide from Aphanothece halaphytica (EPSAH) induced apoptosis in HeLa cells.1932-620310.1371/journal.pone.0087223https://doaj.org/article/985acff01a5044b1a83f8668bb44e8082014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24466342/?tool=EBIhttps://doaj.org/toc/1932-6203The present study aims to investigate the pharmacological effect of the exopolysaccharides from Aphanothece halophytica GR02 (EPSAH) on the HeLa human cervical cancer cell line. HeLa cells were cultured in RPMI-1640-10% FBS medium containing with or without different concentrations of EPSAH. Cell viability was assessed by methylthiazol tetrazolium (MTT) assay. Cell apoptosis was elevated with Wright-Giemsa staining, AO/EB double staining, and DNA fragmentation assay. Apoptosis-associated molecules from cultured HeLa cells were quantified using Western blot analysis. Our results suggest that EPASH induces apoptosis in HeLa cells by targeting a master unfolded protein response (UPR) regulator Grp78. Grp78 further promotes the expression of CHOP and downregulates expression of survivin, which leads to activate mitochondria-mediated downstream molecules and p53-survivin pathway, resulting in caspase-3 activation and causing apoptosis. These findings provide important clues for further evaluating the potential potency of EPSAH for use in cancer therapy.Yu OuShuya XuDandan ZhuXuegan YangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 1, p e87223 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Yu Ou Shuya Xu Dandan Zhu Xuegan Yang Molecular mechanisms of exopolysaccharide from Aphanothece halaphytica (EPSAH) induced apoptosis in HeLa cells.
description	The present study aims to investigate the pharmacological effect of the exopolysaccharides from Aphanothece halophytica GR02 (EPSAH) on the HeLa human cervical cancer cell line. HeLa cells were cultured in RPMI-1640-10% FBS medium containing with or without different concentrations of EPSAH. Cell viability was assessed by methylthiazol tetrazolium (MTT) assay. Cell apoptosis was elevated with Wright-Giemsa staining, AO/EB double staining, and DNA fragmentation assay. Apoptosis-associated molecules from cultured HeLa cells were quantified using Western blot analysis. Our results suggest that EPASH induces apoptosis in HeLa cells by targeting a master unfolded protein response (UPR) regulator Grp78. Grp78 further promotes the expression of CHOP and downregulates expression of survivin, which leads to activate mitochondria-mediated downstream molecules and p53-survivin pathway, resulting in caspase-3 activation and causing apoptosis. These findings provide important clues for further evaluating the potential potency of EPSAH for use in cancer therapy.
format	article
author	Yu Ou Shuya Xu Dandan Zhu Xuegan Yang
author_facet	Yu Ou Shuya Xu Dandan Zhu Xuegan Yang
author_sort	Yu Ou
title	Molecular mechanisms of exopolysaccharide from Aphanothece halaphytica (EPSAH) induced apoptosis in HeLa cells.
title_short	Molecular mechanisms of exopolysaccharide from Aphanothece halaphytica (EPSAH) induced apoptosis in HeLa cells.
title_full	Molecular mechanisms of exopolysaccharide from Aphanothece halaphytica (EPSAH) induced apoptosis in HeLa cells.
title_fullStr	Molecular mechanisms of exopolysaccharide from Aphanothece halaphytica (EPSAH) induced apoptosis in HeLa cells.
title_full_unstemmed	Molecular mechanisms of exopolysaccharide from Aphanothece halaphytica (EPSAH) induced apoptosis in HeLa cells.
title_sort	molecular mechanisms of exopolysaccharide from aphanothece halaphytica (epsah) induced apoptosis in hela cells.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/985acff01a5044b1a83f8668bb44e808
work_keys_str_mv	AT yuou molecularmechanismsofexopolysaccharidefromaphanothecehalaphyticaepsahinducedapoptosisinhelacells AT shuyaxu molecularmechanismsofexopolysaccharidefromaphanothecehalaphyticaepsahinducedapoptosisinhelacells AT dandanzhu molecularmechanismsofexopolysaccharidefromaphanothecehalaphyticaepsahinducedapoptosisinhelacells AT xueganyang molecularmechanismsofexopolysaccharidefromaphanothecehalaphyticaepsahinducedapoptosisinhelacells
_version_	1718421576329723904

Molecular mechanisms of exopolysaccharide from Aphanothece halaphytica (EPSAH) induced apoptosis in HeLa cells.

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