Transcriptome-based analysis of the hormone regulation mechanism of gender differentiation in Juglans mandshurica Maxim

Juglans mandshurica Maxim is a hermaphroditic plant belonging to the genus Juglans in the family Juglandaceae. The pollination period of female flowers is different from the loose powder period of male flowers on the same tree. In several trees, female flowers bloom first, whereas in others, male fl...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Baiting Qin, Xiujun Lu, Xiaomei Sun, Jianguo Cui, Jifeng Deng, Lijie Zhang
Formato:	article
Lenguaje:	EN
Publicado:	PeerJ Inc. 2021
Materias:	Juglans mandshurica maxim Heterodichogamy Transcriptome sequencing Hormonal regulation Medicine R
Acceso en línea:	https://doaj.org/article/986000b682c042b29584046d83660ccb
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:986000b682c042b29584046d83660ccb
record_format	dspace
spelling	oai:doaj.org-article:986000b682c042b29584046d83660ccb2021-11-11T15:05:28ZTranscriptome-based analysis of the hormone regulation mechanism of gender differentiation in Juglans mandshurica Maxim10.7717/peerj.123282167-8359https://doaj.org/article/986000b682c042b29584046d83660ccb2021-11-01T00:00:00Zhttps://peerj.com/articles/12328.pdfhttps://peerj.com/articles/12328/https://doaj.org/toc/2167-8359Juglans mandshurica Maxim is a hermaphroditic plant belonging to the genus Juglans in the family Juglandaceae. The pollination period of female flowers is different from the loose powder period of male flowers on the same tree. In several trees, female flowers bloom first, whereas in others, male flowers bloom first. In this study, male and female flower buds of J. mandshurica at the physiological differentiation stage were used. Illumina-based transcriptome sequencing was performed, and the quality of the sequencing results was evaluated and analyzed. A total of 138,138 unigenes with an average length of 788 bp were obtained. There were 8,116 differentially expressed genes (DEGs); 2,840 genes were upregulated, and 5,276 genes were downregulated. The DEGs were classified by Gene Ontology and analyzed by Kyoto Encyclopedia of Genes and Genomes. The signal transduction factors involved in phytohormone synthesis were selected. The results displayed that ARF and SAUR were expressed differently in the auxin signaling pathway. Additionally, DELLA protein (a negative regulator of gibberellin), the cytokinin synthesis pathway, and A-ARR were downregulated. On April 2nd, the contents of IAA, GA, CTK, ETH and SA in male and female flower buds of two types of J. mandshurica were opposite, and there were obvious genes regulating gender differentiation. Overall, we found that the sex differentiation of J. mandshurica was related to various hormone signal transduction pathways, and hormone signal transduction plays a leading role in regulation.Baiting QinXiujun LuXiaomei SunJianguo CuiJifeng DengLijie ZhangPeerJ Inc.articleJuglans mandshurica maximHeterodichogamyTranscriptome sequencingHormonal regulationMedicineRENPeerJ, Vol 9, p e12328 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Juglans mandshurica maxim Heterodichogamy Transcriptome sequencing Hormonal regulation Medicine R
spellingShingle	Juglans mandshurica maxim Heterodichogamy Transcriptome sequencing Hormonal regulation Medicine R Baiting Qin Xiujun Lu Xiaomei Sun Jianguo Cui Jifeng Deng Lijie Zhang Transcriptome-based analysis of the hormone regulation mechanism of gender differentiation in Juglans mandshurica Maxim
description	Juglans mandshurica Maxim is a hermaphroditic plant belonging to the genus Juglans in the family Juglandaceae. The pollination period of female flowers is different from the loose powder period of male flowers on the same tree. In several trees, female flowers bloom first, whereas in others, male flowers bloom first. In this study, male and female flower buds of J. mandshurica at the physiological differentiation stage were used. Illumina-based transcriptome sequencing was performed, and the quality of the sequencing results was evaluated and analyzed. A total of 138,138 unigenes with an average length of 788 bp were obtained. There were 8,116 differentially expressed genes (DEGs); 2,840 genes were upregulated, and 5,276 genes were downregulated. The DEGs were classified by Gene Ontology and analyzed by Kyoto Encyclopedia of Genes and Genomes. The signal transduction factors involved in phytohormone synthesis were selected. The results displayed that ARF and SAUR were expressed differently in the auxin signaling pathway. Additionally, DELLA protein (a negative regulator of gibberellin), the cytokinin synthesis pathway, and A-ARR were downregulated. On April 2nd, the contents of IAA, GA, CTK, ETH and SA in male and female flower buds of two types of J. mandshurica were opposite, and there were obvious genes regulating gender differentiation. Overall, we found that the sex differentiation of J. mandshurica was related to various hormone signal transduction pathways, and hormone signal transduction plays a leading role in regulation.
format	article
author	Baiting Qin Xiujun Lu Xiaomei Sun Jianguo Cui Jifeng Deng Lijie Zhang
author_facet	Baiting Qin Xiujun Lu Xiaomei Sun Jianguo Cui Jifeng Deng Lijie Zhang
author_sort	Baiting Qin
title	Transcriptome-based analysis of the hormone regulation mechanism of gender differentiation in Juglans mandshurica Maxim
title_short	Transcriptome-based analysis of the hormone regulation mechanism of gender differentiation in Juglans mandshurica Maxim
title_full	Transcriptome-based analysis of the hormone regulation mechanism of gender differentiation in Juglans mandshurica Maxim
title_fullStr	Transcriptome-based analysis of the hormone regulation mechanism of gender differentiation in Juglans mandshurica Maxim
title_full_unstemmed	Transcriptome-based analysis of the hormone regulation mechanism of gender differentiation in Juglans mandshurica Maxim
title_sort	transcriptome-based analysis of the hormone regulation mechanism of gender differentiation in juglans mandshurica maxim
publisher	PeerJ Inc.
publishDate	2021
url	https://doaj.org/article/986000b682c042b29584046d83660ccb
work_keys_str_mv	AT baitingqin transcriptomebasedanalysisofthehormoneregulationmechanismofgenderdifferentiationinjuglansmandshuricamaxim AT xiujunlu transcriptomebasedanalysisofthehormoneregulationmechanismofgenderdifferentiationinjuglansmandshuricamaxim AT xiaomeisun transcriptomebasedanalysisofthehormoneregulationmechanismofgenderdifferentiationinjuglansmandshuricamaxim AT jianguocui transcriptomebasedanalysisofthehormoneregulationmechanismofgenderdifferentiationinjuglansmandshuricamaxim AT jifengdeng transcriptomebasedanalysisofthehormoneregulationmechanismofgenderdifferentiationinjuglansmandshuricamaxim AT lijiezhang transcriptomebasedanalysisofthehormoneregulationmechanismofgenderdifferentiationinjuglansmandshuricamaxim
_version_	1718437159845756928

Transcriptome-based analysis of the hormone regulation mechanism of gender differentiation in Juglans mandshurica Maxim

Ejemplares similares