Composition and function of the C1b/C1f region in the ciliary central apparatus

Abstract Motile cilia are ultrastructurally complex cell organelles with the ability to actively move. The highly conserved central apparatus of motile 9 × 2 + 2 cilia is composed of two microtubules and several large microtubule-bound projections, including the C1b/C1f supercomplex. The composition...

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Autores principales:	Ewa Joachimiak, Anna Osinka, Hanan Farahat, Bianka Świderska, Ewa Sitkiewicz, Martyna Poprzeczko, Hanna Fabczak, Dorota Wloga
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/9861cc43595b45b9b2d7045943a7c04b
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spelling	oai:doaj.org-article:9861cc43595b45b9b2d7045943a7c04b2021-12-02T15:02:40ZComposition and function of the C1b/C1f region in the ciliary central apparatus10.1038/s41598-021-90996-92045-2322https://doaj.org/article/9861cc43595b45b9b2d7045943a7c04b2021-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90996-9https://doaj.org/toc/2045-2322Abstract Motile cilia are ultrastructurally complex cell organelles with the ability to actively move. The highly conserved central apparatus of motile 9 × 2 + 2 cilia is composed of two microtubules and several large microtubule-bound projections, including the C1b/C1f supercomplex. The composition and function of C1b/C1f subunits has only recently started to emerge. We show that in the model ciliate Tetrahymena thermophila, C1b/C1f contains several evolutionarily conserved proteins: Spef2A, Cfap69, Cfap246/LRGUK, Adgb/androglobin, and a ciliate-specific protein Tt170/TTHERM_00205170. Deletion of genes encoding either Spef2A or Cfap69 led to a loss of the entire C1b projection and resulted in an abnormal vortex motion of cilia. Loss of either Cfap246 or Adgb caused only minor alterations in ciliary motility. Comparative analyses of wild-type and C1b-deficient mutant ciliomes revealed that the levels of subunits forming the adjacent C2b projection but not C1d projection are greatly reduced, indicating that C1b stabilizes C2b. Moreover, the levels of several IFT and BBS proteins, HSP70, and enzymes that catalyze the final steps of the glycolytic pathway: enolase ENO1 and pyruvate kinase PYK1, are also reduced in the C1b-less mutants.Ewa JoachimiakAnna OsinkaHanan FarahatBianka ŚwiderskaEwa SitkiewiczMartyna PoprzeczkoHanna FabczakDorota WlogaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ewa Joachimiak Anna Osinka Hanan Farahat Bianka Świderska Ewa Sitkiewicz Martyna Poprzeczko Hanna Fabczak Dorota Wloga Composition and function of the C1b/C1f region in the ciliary central apparatus
description	Abstract Motile cilia are ultrastructurally complex cell organelles with the ability to actively move. The highly conserved central apparatus of motile 9 × 2 + 2 cilia is composed of two microtubules and several large microtubule-bound projections, including the C1b/C1f supercomplex. The composition and function of C1b/C1f subunits has only recently started to emerge. We show that in the model ciliate Tetrahymena thermophila, C1b/C1f contains several evolutionarily conserved proteins: Spef2A, Cfap69, Cfap246/LRGUK, Adgb/androglobin, and a ciliate-specific protein Tt170/TTHERM_00205170. Deletion of genes encoding either Spef2A or Cfap69 led to a loss of the entire C1b projection and resulted in an abnormal vortex motion of cilia. Loss of either Cfap246 or Adgb caused only minor alterations in ciliary motility. Comparative analyses of wild-type and C1b-deficient mutant ciliomes revealed that the levels of subunits forming the adjacent C2b projection but not C1d projection are greatly reduced, indicating that C1b stabilizes C2b. Moreover, the levels of several IFT and BBS proteins, HSP70, and enzymes that catalyze the final steps of the glycolytic pathway: enolase ENO1 and pyruvate kinase PYK1, are also reduced in the C1b-less mutants.
format	article
author	Ewa Joachimiak Anna Osinka Hanan Farahat Bianka Świderska Ewa Sitkiewicz Martyna Poprzeczko Hanna Fabczak Dorota Wloga
author_facet	Ewa Joachimiak Anna Osinka Hanan Farahat Bianka Świderska Ewa Sitkiewicz Martyna Poprzeczko Hanna Fabczak Dorota Wloga
author_sort	Ewa Joachimiak
title	Composition and function of the C1b/C1f region in the ciliary central apparatus
title_short	Composition and function of the C1b/C1f region in the ciliary central apparatus
title_full	Composition and function of the C1b/C1f region in the ciliary central apparatus
title_fullStr	Composition and function of the C1b/C1f region in the ciliary central apparatus
title_full_unstemmed	Composition and function of the C1b/C1f region in the ciliary central apparatus
title_sort	composition and function of the c1b/c1f region in the ciliary central apparatus
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/9861cc43595b45b9b2d7045943a7c04b
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Composition and function of the C1b/C1f region in the ciliary central apparatus

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