Discovering the Potent Inhibitors Against Babesia bovis in vitro and Babesia microti in vivo by Repurposing the Natural Product Compounds

In the present study, we screened 502 natural product compounds against the in vitro growth of Babesia (B.) bovis. Then, the novel and potent identified compounds were further evaluated for their in vitro efficacies using viability and cytotoxicity assays. The in vivo inhibitory effects of the selec...

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Autores principales:	Yongchang Li, Mohamed Abdo Rizk, Eloiza May Galon, Mingming Liu, Jixu Li, Aaron Edmond Ringo, Shengwei Ji, Iqra Zafar, Maria Agnes Tumwebaze, Byamukama Benedicto, Naoaki Yokoyama, Ikuo Igarashi, Bayin Chahan, Xuenan Xuan
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	natural product compounds Babesia bovis in vitro Babesia microti in vivo Veterinary medicine SF600-1100
Acceso en línea:	https://doaj.org/article/986906b003574c5ca593d357711b9414
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spelling	oai:doaj.org-article:986906b003574c5ca593d357711b94142021-12-01T13:09:46ZDiscovering the Potent Inhibitors Against Babesia bovis in vitro and Babesia microti in vivo by Repurposing the Natural Product Compounds2297-176910.3389/fvets.2021.762107https://doaj.org/article/986906b003574c5ca593d357711b94142021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fvets.2021.762107/fullhttps://doaj.org/toc/2297-1769In the present study, we screened 502 natural product compounds against the in vitro growth of Babesia (B.) bovis. Then, the novel and potent identified compounds were further evaluated for their in vitro efficacies using viability and cytotoxicity assays. The in vivo inhibitory effects of the selected compounds were evaluated using B. microti “rodent strain” in mice model. Three potent compounds, namely, Rottlerin (RL), Narasin (NR), Lasalocid acid (LA), exhibited the lowest IC50 (half-maximal inhibitory concentration) as follows: 5.45 ± 1.20 μM for RL, 1.86 ± 0.66 μM for NR, and 3.56 ± 1.41 μM for LA. The viability result revealed the ability of RL and LA to prevent the regrowth of treated parasite at 4 × IC50 and 2 × IC50, respectively, while 4 × IC50 of NR was sufficient to stop the regrowth of parasite. The hematology parameters of B. microti in vivo were different in the NR-treated groups as compared to the infected/untreated group. Interestingly, intraperitoneal administration of NR exhibiting inhibition in the growth of B. microti in mice was similar to that observed after administration of the commonly used antibabesial drug, diminazene aceturate (DA) (76.57% for DA, 74.73% for NR). Our findings indicate the richness of natural product compounds by novel potent antibabesial candidates, and the identified potent compounds, especially NR, might be used for the treatment of animal babesiosis.Yongchang LiYongchang LiMohamed Abdo RizkMohamed Abdo RizkEloiza May GalonMingming LiuMingming LiuJixu LiJixu LiAaron Edmond RingoShengwei JiIqra ZafarMaria Agnes TumwebazeByamukama BenedictoNaoaki YokoyamaIkuo IgarashiBayin ChahanXuenan XuanFrontiers Media S.A.articlenatural product compoundsBabesia bovisin vitroBabesia microtiin vivoVeterinary medicineSF600-1100ENFrontiers in Veterinary Science, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	natural product compounds Babesia bovis in vitro Babesia microti in vivo Veterinary medicine SF600-1100
spellingShingle	natural product compounds Babesia bovis in vitro Babesia microti in vivo Veterinary medicine SF600-1100 Yongchang Li Yongchang Li Mohamed Abdo Rizk Mohamed Abdo Rizk Eloiza May Galon Mingming Liu Mingming Liu Jixu Li Jixu Li Aaron Edmond Ringo Shengwei Ji Iqra Zafar Maria Agnes Tumwebaze Byamukama Benedicto Naoaki Yokoyama Ikuo Igarashi Bayin Chahan Xuenan Xuan Discovering the Potent Inhibitors Against Babesia bovis in vitro and Babesia microti in vivo by Repurposing the Natural Product Compounds
description	In the present study, we screened 502 natural product compounds against the in vitro growth of Babesia (B.) bovis. Then, the novel and potent identified compounds were further evaluated for their in vitro efficacies using viability and cytotoxicity assays. The in vivo inhibitory effects of the selected compounds were evaluated using B. microti “rodent strain” in mice model. Three potent compounds, namely, Rottlerin (RL), Narasin (NR), Lasalocid acid (LA), exhibited the lowest IC50 (half-maximal inhibitory concentration) as follows: 5.45 ± 1.20 μM for RL, 1.86 ± 0.66 μM for NR, and 3.56 ± 1.41 μM for LA. The viability result revealed the ability of RL and LA to prevent the regrowth of treated parasite at 4 × IC50 and 2 × IC50, respectively, while 4 × IC50 of NR was sufficient to stop the regrowth of parasite. The hematology parameters of B. microti in vivo were different in the NR-treated groups as compared to the infected/untreated group. Interestingly, intraperitoneal administration of NR exhibiting inhibition in the growth of B. microti in mice was similar to that observed after administration of the commonly used antibabesial drug, diminazene aceturate (DA) (76.57% for DA, 74.73% for NR). Our findings indicate the richness of natural product compounds by novel potent antibabesial candidates, and the identified potent compounds, especially NR, might be used for the treatment of animal babesiosis.
format	article
author	Yongchang Li Yongchang Li Mohamed Abdo Rizk Mohamed Abdo Rizk Eloiza May Galon Mingming Liu Mingming Liu Jixu Li Jixu Li Aaron Edmond Ringo Shengwei Ji Iqra Zafar Maria Agnes Tumwebaze Byamukama Benedicto Naoaki Yokoyama Ikuo Igarashi Bayin Chahan Xuenan Xuan
author_facet	Yongchang Li Yongchang Li Mohamed Abdo Rizk Mohamed Abdo Rizk Eloiza May Galon Mingming Liu Mingming Liu Jixu Li Jixu Li Aaron Edmond Ringo Shengwei Ji Iqra Zafar Maria Agnes Tumwebaze Byamukama Benedicto Naoaki Yokoyama Ikuo Igarashi Bayin Chahan Xuenan Xuan
author_sort	Yongchang Li
title	Discovering the Potent Inhibitors Against Babesia bovis in vitro and Babesia microti in vivo by Repurposing the Natural Product Compounds
title_short	Discovering the Potent Inhibitors Against Babesia bovis in vitro and Babesia microti in vivo by Repurposing the Natural Product Compounds
title_full	Discovering the Potent Inhibitors Against Babesia bovis in vitro and Babesia microti in vivo by Repurposing the Natural Product Compounds
title_fullStr	Discovering the Potent Inhibitors Against Babesia bovis in vitro and Babesia microti in vivo by Repurposing the Natural Product Compounds
title_full_unstemmed	Discovering the Potent Inhibitors Against Babesia bovis in vitro and Babesia microti in vivo by Repurposing the Natural Product Compounds
title_sort	discovering the potent inhibitors against babesia bovis in vitro and babesia microti in vivo by repurposing the natural product compounds
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/986906b003574c5ca593d357711b9414
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Discovering the Potent Inhibitors Against Babesia bovis in vitro and Babesia microti in vivo by Repurposing the Natural Product Compounds

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