Evaluation of Antibacterial Effects of Matrix-Induced Silver Ions against Antibiotic-Resistant ESKAPE Pathogens

Recently, drug-resistant bacterial infections, especially ESKAPE pathogens (<i>Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter</i> spp.), have become a critical health issue worldwide, highlighting the...

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Autores principales:	Ya-Chi Huang, Tsung-Ying Yang, Bo-Xuan Chen, Jung-Chang Kung, Chi-Jen Shih
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	silver-containing mesoporous bioactive glass matrix induction silver ion antibacterial agent methicillin resistance vancomycin resistance Medicine R Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/986aea962b8442a6a9c62356d60bd777
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spelling	oai:doaj.org-article:986aea962b8442a6a9c62356d60bd7772021-11-25T18:39:18ZEvaluation of Antibacterial Effects of Matrix-Induced Silver Ions against Antibiotic-Resistant ESKAPE Pathogens10.3390/ph141110941424-8247https://doaj.org/article/986aea962b8442a6a9c62356d60bd7772021-10-01T00:00:00Zhttps://www.mdpi.com/1424-8247/14/11/1094https://doaj.org/toc/1424-8247Recently, drug-resistant bacterial infections, especially ESKAPE pathogens (<i>Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter</i> spp.), have become a critical health issue worldwide, highlighting the emerging need for novel antibacterial agents. In this study, silver nanoparticles were extracted from silver-containing mesoporous bioactive glass (MBG-Ag) using four different matrixes, including water, phosphate buffer saline (PBS), tryptic soy broth (TSB), and taurine (Tau). The inductively coupled plasma-mass spectrometer (ICP-MS) results demonstrated that the silver concentration of Tau-Ag was the highest among the four matrixes. The Tau-Ag was also observed to have 87.35% silver ions in its X-ray photoelectron spectrometer (XPS) spectra. The micrograph of transmission electron microscope (TEM) displayed a uniform distribution of silver nanoparticles, which was confined in a smaller size compared to that in TSB-Ag. Moreover, the peak shifts observed in the Fourier-transform infrared spectrometer (FTIR) spectrum implied that the -SO<sub>3</sub><sup>2−</sup> and -NH groups in taurine may interact with silver. A low cytotoxicity was noted for Tau-Ag, with approximately 70% of cells surviving at 0.63 mg/mL. Compared to the other three matrix-induced silver agents, Tau-Ag represented a better antibacterial effect against methicillin-resistant <i>Staphylococcus aureus</i>, with a minimum inhibitory concentration (MIC) value of 0.63 mg/mL and a postponed growth of 0.31 mg/mL observed. Further antibacterial examinations illustrated the presence of remarkable antibacterial activities against vancomycin-resistant <i>Enterococcus feacium</i>, carbapenem-resistant <i>Klebsiella pneumoniae</i>, carbapenem-resistant <i>Acinetobacter baumannii</i>, and carbapenem-resistant <i>Pseudomonas aeruginosa</i>. Given our observations and multiple bioactive functions of taurine (prevent patients from inflammation and oxidative-stress injuries), we anticipate that taurine matrix-induced silver ions would be a biomedical material with a high potential for combatting drug-resistant ESKAPE pathogens.Ya-Chi HuangTsung-Ying YangBo-Xuan ChenJung-Chang KungChi-Jen ShihMDPI AGarticlesilver-containing mesoporous bioactive glassmatrix inductionsilver ionantibacterial agentmethicillin resistancevancomycin resistanceMedicineRPharmacy and materia medicaRS1-441ENPharmaceuticals, Vol 14, Iss 1094, p 1094 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	silver-containing mesoporous bioactive glass matrix induction silver ion antibacterial agent methicillin resistance vancomycin resistance Medicine R Pharmacy and materia medica RS1-441
spellingShingle	silver-containing mesoporous bioactive glass matrix induction silver ion antibacterial agent methicillin resistance vancomycin resistance Medicine R Pharmacy and materia medica RS1-441 Ya-Chi Huang Tsung-Ying Yang Bo-Xuan Chen Jung-Chang Kung Chi-Jen Shih Evaluation of Antibacterial Effects of Matrix-Induced Silver Ions against Antibiotic-Resistant ESKAPE Pathogens
description	Recently, drug-resistant bacterial infections, especially ESKAPE pathogens (<i>Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter</i> spp.), have become a critical health issue worldwide, highlighting the emerging need for novel antibacterial agents. In this study, silver nanoparticles were extracted from silver-containing mesoporous bioactive glass (MBG-Ag) using four different matrixes, including water, phosphate buffer saline (PBS), tryptic soy broth (TSB), and taurine (Tau). The inductively coupled plasma-mass spectrometer (ICP-MS) results demonstrated that the silver concentration of Tau-Ag was the highest among the four matrixes. The Tau-Ag was also observed to have 87.35% silver ions in its X-ray photoelectron spectrometer (XPS) spectra. The micrograph of transmission electron microscope (TEM) displayed a uniform distribution of silver nanoparticles, which was confined in a smaller size compared to that in TSB-Ag. Moreover, the peak shifts observed in the Fourier-transform infrared spectrometer (FTIR) spectrum implied that the -SO<sub>3</sub><sup>2−</sup> and -NH groups in taurine may interact with silver. A low cytotoxicity was noted for Tau-Ag, with approximately 70% of cells surviving at 0.63 mg/mL. Compared to the other three matrix-induced silver agents, Tau-Ag represented a better antibacterial effect against methicillin-resistant <i>Staphylococcus aureus</i>, with a minimum inhibitory concentration (MIC) value of 0.63 mg/mL and a postponed growth of 0.31 mg/mL observed. Further antibacterial examinations illustrated the presence of remarkable antibacterial activities against vancomycin-resistant <i>Enterococcus feacium</i>, carbapenem-resistant <i>Klebsiella pneumoniae</i>, carbapenem-resistant <i>Acinetobacter baumannii</i>, and carbapenem-resistant <i>Pseudomonas aeruginosa</i>. Given our observations and multiple bioactive functions of taurine (prevent patients from inflammation and oxidative-stress injuries), we anticipate that taurine matrix-induced silver ions would be a biomedical material with a high potential for combatting drug-resistant ESKAPE pathogens.
format	article
author	Ya-Chi Huang Tsung-Ying Yang Bo-Xuan Chen Jung-Chang Kung Chi-Jen Shih
author_facet	Ya-Chi Huang Tsung-Ying Yang Bo-Xuan Chen Jung-Chang Kung Chi-Jen Shih
author_sort	Ya-Chi Huang
title	Evaluation of Antibacterial Effects of Matrix-Induced Silver Ions against Antibiotic-Resistant ESKAPE Pathogens
title_short	Evaluation of Antibacterial Effects of Matrix-Induced Silver Ions against Antibiotic-Resistant ESKAPE Pathogens
title_full	Evaluation of Antibacterial Effects of Matrix-Induced Silver Ions against Antibiotic-Resistant ESKAPE Pathogens
title_fullStr	Evaluation of Antibacterial Effects of Matrix-Induced Silver Ions against Antibiotic-Resistant ESKAPE Pathogens
title_full_unstemmed	Evaluation of Antibacterial Effects of Matrix-Induced Silver Ions against Antibiotic-Resistant ESKAPE Pathogens
title_sort	evaluation of antibacterial effects of matrix-induced silver ions against antibiotic-resistant eskape pathogens
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/986aea962b8442a6a9c62356d60bd777
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Evaluation of Antibacterial Effects of Matrix-Induced Silver Ions against Antibiotic-Resistant ESKAPE Pathogens

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