Effect of Berberine Hydrochloride Treatment on Transcriptomic Response in Gill Tissue of Charybdis japonica Infected With Aeromonas hydrophila

Berberine hydrochloride is the main active ingredient of Coptis chinensis, which has demonstrated significant antibacterial activity against bacteria. However, the molecular mechanism underlying its effects is unclear in crabs. In this study, we used transcriptome sequencing to investigate the molec...

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Autores principales:	Mingming Han, Yuxin Liu, Tongqing Zhang, Daming Li, Qichen Jiang, Zakaria Zuraini
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	Charybdis japonica berberine hydrochloride Aeromonas hydrophila transcriptome immune mechanisms Science Q General. Including nature conservation, geographical distribution QH1-199.5
Acceso en línea:	https://doaj.org/article/986fd48a7bd4417d89743baea0c14ce3
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spelling	oai:doaj.org-article:986fd48a7bd4417d89743baea0c14ce32021-11-16T04:45:12ZEffect of Berberine Hydrochloride Treatment on Transcriptomic Response in Gill Tissue of Charybdis japonica Infected With Aeromonas hydrophila2296-774510.3389/fmars.2021.754588https://doaj.org/article/986fd48a7bd4417d89743baea0c14ce32021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmars.2021.754588/fullhttps://doaj.org/toc/2296-7745Berberine hydrochloride is the main active ingredient of Coptis chinensis, which has demonstrated significant antibacterial activity against bacteria. However, the molecular mechanism underlying its effects is unclear in crabs. In this study, we used transcriptome sequencing to investigate the molecules involved in the therapeutic and defensive responses of Charybdis japonica infected with 105 colony-forming units (CFU)/L of Aeromonas hydrophila following treatment with berberine hydrochloride. A total of 5,409 differentially expressed genes were observed after exposure of C. japonicus for 72 h to 100 mg/L berberine hydrochloride, 100 mg/L berberine hydrochloride plus injection of 105 CFU/L A. hydrophila, and a control group injected with equal amount of physiological saline solution. Enrichment analysis revealed that these genes were involved in metabolism, cellular processes, signal transduction, and immune function. The transcriptomic results indicated that exposure to berberine hydrochloride activated glutathione metabolism, oxidative phosphorylation, fatty acid degradation, retinol metabolism, pyruvate metabolism, and the hypoxia-inducible factor 1 signaling pathway. Transcriptomic analysis and relative mRNA level analysis also identified acid hydrolase genes and phagosomal genes as key factors regulating the adaptation of acid berberine-infused C. japonicus to infection with A. hydrophila, Based on the ability of berberine hydrochloride to induce acid hydrolase and phagosomal gene expression, which can in turn remove bacteria, immersion in berberine hydrochloride may remove A. hydrophila and thus improve the survival of C. japonica. The results of this study provided a new scientific basis for the potential role of berberine hydrochloride in the immune mechanisms of crabs.Mingming HanYuxin LiuTongqing ZhangDaming LiQichen JiangZakaria ZurainiFrontiers Media S.A.articleCharybdis japonicaberberine hydrochlorideAeromonas hydrophilatranscriptomeimmune mechanismsScienceQGeneral. Including nature conservation, geographical distributionQH1-199.5ENFrontiers in Marine Science, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Charybdis japonica berberine hydrochloride Aeromonas hydrophila transcriptome immune mechanisms Science Q General. Including nature conservation, geographical distribution QH1-199.5
spellingShingle	Charybdis japonica berberine hydrochloride Aeromonas hydrophila transcriptome immune mechanisms Science Q General. Including nature conservation, geographical distribution QH1-199.5 Mingming Han Yuxin Liu Tongqing Zhang Daming Li Qichen Jiang Zakaria Zuraini Effect of Berberine Hydrochloride Treatment on Transcriptomic Response in Gill Tissue of Charybdis japonica Infected With Aeromonas hydrophila
description	Berberine hydrochloride is the main active ingredient of Coptis chinensis, which has demonstrated significant antibacterial activity against bacteria. However, the molecular mechanism underlying its effects is unclear in crabs. In this study, we used transcriptome sequencing to investigate the molecules involved in the therapeutic and defensive responses of Charybdis japonica infected with 105 colony-forming units (CFU)/L of Aeromonas hydrophila following treatment with berberine hydrochloride. A total of 5,409 differentially expressed genes were observed after exposure of C. japonicus for 72 h to 100 mg/L berberine hydrochloride, 100 mg/L berberine hydrochloride plus injection of 105 CFU/L A. hydrophila, and a control group injected with equal amount of physiological saline solution. Enrichment analysis revealed that these genes were involved in metabolism, cellular processes, signal transduction, and immune function. The transcriptomic results indicated that exposure to berberine hydrochloride activated glutathione metabolism, oxidative phosphorylation, fatty acid degradation, retinol metabolism, pyruvate metabolism, and the hypoxia-inducible factor 1 signaling pathway. Transcriptomic analysis and relative mRNA level analysis also identified acid hydrolase genes and phagosomal genes as key factors regulating the adaptation of acid berberine-infused C. japonicus to infection with A. hydrophila, Based on the ability of berberine hydrochloride to induce acid hydrolase and phagosomal gene expression, which can in turn remove bacteria, immersion in berberine hydrochloride may remove A. hydrophila and thus improve the survival of C. japonica. The results of this study provided a new scientific basis for the potential role of berberine hydrochloride in the immune mechanisms of crabs.
format	article
author	Mingming Han Yuxin Liu Tongqing Zhang Daming Li Qichen Jiang Zakaria Zuraini
author_facet	Mingming Han Yuxin Liu Tongqing Zhang Daming Li Qichen Jiang Zakaria Zuraini
author_sort	Mingming Han
title	Effect of Berberine Hydrochloride Treatment on Transcriptomic Response in Gill Tissue of Charybdis japonica Infected With Aeromonas hydrophila
title_short	Effect of Berberine Hydrochloride Treatment on Transcriptomic Response in Gill Tissue of Charybdis japonica Infected With Aeromonas hydrophila
title_full	Effect of Berberine Hydrochloride Treatment on Transcriptomic Response in Gill Tissue of Charybdis japonica Infected With Aeromonas hydrophila
title_fullStr	Effect of Berberine Hydrochloride Treatment on Transcriptomic Response in Gill Tissue of Charybdis japonica Infected With Aeromonas hydrophila
title_full_unstemmed	Effect of Berberine Hydrochloride Treatment on Transcriptomic Response in Gill Tissue of Charybdis japonica Infected With Aeromonas hydrophila
title_sort	effect of berberine hydrochloride treatment on transcriptomic response in gill tissue of charybdis japonica infected with aeromonas hydrophila
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/986fd48a7bd4417d89743baea0c14ce3
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Effect of Berberine Hydrochloride Treatment on Transcriptomic Response in Gill Tissue of Charybdis japonica Infected With Aeromonas hydrophila

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