Transcriptomic, biochemical and physio-anatomical investigations shed more light on responses to drought stress in two contrasting sesame genotypes

Abstract Sesame is an important oilseed crop with a high oil quality. It is prone to drought stress in the arid and semi-arid areas where it is widely grown. This study aims to decipher the response of tolerant (DT) and sensitive (DS) genotypes to progressive drought based on transcriptome, biochemi...

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Autores principales:	Komivi Dossa, Donghua Li, Linhai Wang, Xiaomin Zheng, Aili Liu, Jingyin Yu, Xin Wei, Rong Zhou, Daniel Fonceka, Diaga Diouf, Boshou Liao, Ndiaga Cissé, Xiurong Zhang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/98725da3e49e43f88ce9ecdd871f8ede
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spelling	oai:doaj.org-article:98725da3e49e43f88ce9ecdd871f8ede2021-12-02T16:06:37ZTranscriptomic, biochemical and physio-anatomical investigations shed more light on responses to drought stress in two contrasting sesame genotypes10.1038/s41598-017-09397-62045-2322https://doaj.org/article/98725da3e49e43f88ce9ecdd871f8ede2017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-09397-6https://doaj.org/toc/2045-2322Abstract Sesame is an important oilseed crop with a high oil quality. It is prone to drought stress in the arid and semi-arid areas where it is widely grown. This study aims to decipher the response of tolerant (DT) and sensitive (DS) genotypes to progressive drought based on transcriptome, biochemical and physio-anatomical characterizations. Results indicated that under severe stress, DT relied on a well-functioning taproot while DS displayed a disintegrated root due to collapsed cortical cells. This was attributed to a higher accumulation of osmoprotectants and strong activity of antioxidant enzymes especially peroxidases in DT. From roots, DT could supply water to the aboveground tissues to ensure photosynthetic activities and improve endurance under stress. Temporal transcriptome sequencing under drought further confirmed that DT strongly activated genes related to antioxidant activity, osmoprotection and hormonal signaling pathways including abscisic acid and Ethylene. Furthermore, DT displayed unique differentially expressed genes in root functioning as peroxidases, interleukin receptor-associated kinase, heat shock proteins, APETALA2/ethylene-responsive element-binding protein and mitogen activated protein kinase, to effectively scavenge reactive oxygen species and preserve root cell integrity. Finally, 61 candidate genes conferring higher drought tolerance in DT were discovered and may constitute useful resources for drought tolerance improvement in sesame.Komivi DossaDonghua LiLinhai WangXiaomin ZhengAili LiuJingyin YuXin WeiRong ZhouDaniel FoncekaDiaga DioufBoshou LiaoNdiaga CisséXiurong ZhangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-14 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Komivi Dossa Donghua Li Linhai Wang Xiaomin Zheng Aili Liu Jingyin Yu Xin Wei Rong Zhou Daniel Fonceka Diaga Diouf Boshou Liao Ndiaga Cissé Xiurong Zhang Transcriptomic, biochemical and physio-anatomical investigations shed more light on responses to drought stress in two contrasting sesame genotypes
description	Abstract Sesame is an important oilseed crop with a high oil quality. It is prone to drought stress in the arid and semi-arid areas where it is widely grown. This study aims to decipher the response of tolerant (DT) and sensitive (DS) genotypes to progressive drought based on transcriptome, biochemical and physio-anatomical characterizations. Results indicated that under severe stress, DT relied on a well-functioning taproot while DS displayed a disintegrated root due to collapsed cortical cells. This was attributed to a higher accumulation of osmoprotectants and strong activity of antioxidant enzymes especially peroxidases in DT. From roots, DT could supply water to the aboveground tissues to ensure photosynthetic activities and improve endurance under stress. Temporal transcriptome sequencing under drought further confirmed that DT strongly activated genes related to antioxidant activity, osmoprotection and hormonal signaling pathways including abscisic acid and Ethylene. Furthermore, DT displayed unique differentially expressed genes in root functioning as peroxidases, interleukin receptor-associated kinase, heat shock proteins, APETALA2/ethylene-responsive element-binding protein and mitogen activated protein kinase, to effectively scavenge reactive oxygen species and preserve root cell integrity. Finally, 61 candidate genes conferring higher drought tolerance in DT were discovered and may constitute useful resources for drought tolerance improvement in sesame.
format	article
author	Komivi Dossa Donghua Li Linhai Wang Xiaomin Zheng Aili Liu Jingyin Yu Xin Wei Rong Zhou Daniel Fonceka Diaga Diouf Boshou Liao Ndiaga Cissé Xiurong Zhang
author_facet	Komivi Dossa Donghua Li Linhai Wang Xiaomin Zheng Aili Liu Jingyin Yu Xin Wei Rong Zhou Daniel Fonceka Diaga Diouf Boshou Liao Ndiaga Cissé Xiurong Zhang
author_sort	Komivi Dossa
title	Transcriptomic, biochemical and physio-anatomical investigations shed more light on responses to drought stress in two contrasting sesame genotypes
title_short	Transcriptomic, biochemical and physio-anatomical investigations shed more light on responses to drought stress in two contrasting sesame genotypes
title_full	Transcriptomic, biochemical and physio-anatomical investigations shed more light on responses to drought stress in two contrasting sesame genotypes
title_fullStr	Transcriptomic, biochemical and physio-anatomical investigations shed more light on responses to drought stress in two contrasting sesame genotypes
title_full_unstemmed	Transcriptomic, biochemical and physio-anatomical investigations shed more light on responses to drought stress in two contrasting sesame genotypes
title_sort	transcriptomic, biochemical and physio-anatomical investigations shed more light on responses to drought stress in two contrasting sesame genotypes
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/98725da3e49e43f88ce9ecdd871f8ede
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Transcriptomic, biochemical and physio-anatomical investigations shed more light on responses to drought stress in two contrasting sesame genotypes

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