Identification of the antibacterial mechanism of cryptotanshinone on methicillin-resistant Staphylococcus aureus using bioinformatics analysis

Abstract Cryptotanshinone (CT) is an extract from the traditional Chinese medicine Salvia miltiorrhiza, which inhibits the growth of methicillin-resistant Staphylococcus aureus (MRSA) in vitro. This study aims to determine the antibacterial mechanisms of CT by integrating bioinformatics analysis and...

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Autores principales:	Jiwei Zhong, Haidan Wang, Yun Zhuang, Qun Shen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/987422a4ec194de4b118c71328119011
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spelling	oai:doaj.org-article:987422a4ec194de4b118c713281190112021-11-08T10:55:29ZIdentification of the antibacterial mechanism of cryptotanshinone on methicillin-resistant Staphylococcus aureus using bioinformatics analysis10.1038/s41598-021-01121-92045-2322https://doaj.org/article/987422a4ec194de4b118c713281190112021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01121-9https://doaj.org/toc/2045-2322Abstract Cryptotanshinone (CT) is an extract from the traditional Chinese medicine Salvia miltiorrhiza, which inhibits the growth of methicillin-resistant Staphylococcus aureus (MRSA) in vitro. This study aims to determine the antibacterial mechanisms of CT by integrating bioinformatics analysis and microbiology assay. The microarray data of GSE13203 was retrieved from the Gene Expression Omnibus (GEO) database to screen the differentially expressed genes (DEGs) of S. aureus strains that were treated with CT treatment. Gene ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were used to identify the potential target of CT. Data mining on the microarray dataset indicated that pyruvate kinase (PK) might be involved in the antimicrobial activities of CT. The minimum inhibition concentrations (MICs) of CT or vancomycin against the MRSA strain ATCC43300 and seven other clinical strains were determined using the broth dilution method. The effects of CT on the activity of PK were further measured. In vitro tests verified that CT inhibited the growth of an MRSA reference strain and seven other clinical strains. CT hampered the activity of the PK of ATCC43300 and five clinical MRSA strains. CT might hinder bacterial energy metabolism by inhibiting the activity of PK.Jiwei ZhongHaidan WangYun ZhuangQun ShenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jiwei Zhong Haidan Wang Yun Zhuang Qun Shen Identification of the antibacterial mechanism of cryptotanshinone on methicillin-resistant Staphylococcus aureus using bioinformatics analysis
description	Abstract Cryptotanshinone (CT) is an extract from the traditional Chinese medicine Salvia miltiorrhiza, which inhibits the growth of methicillin-resistant Staphylococcus aureus (MRSA) in vitro. This study aims to determine the antibacterial mechanisms of CT by integrating bioinformatics analysis and microbiology assay. The microarray data of GSE13203 was retrieved from the Gene Expression Omnibus (GEO) database to screen the differentially expressed genes (DEGs) of S. aureus strains that were treated with CT treatment. Gene ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were used to identify the potential target of CT. Data mining on the microarray dataset indicated that pyruvate kinase (PK) might be involved in the antimicrobial activities of CT. The minimum inhibition concentrations (MICs) of CT or vancomycin against the MRSA strain ATCC43300 and seven other clinical strains were determined using the broth dilution method. The effects of CT on the activity of PK were further measured. In vitro tests verified that CT inhibited the growth of an MRSA reference strain and seven other clinical strains. CT hampered the activity of the PK of ATCC43300 and five clinical MRSA strains. CT might hinder bacterial energy metabolism by inhibiting the activity of PK.
format	article
author	Jiwei Zhong Haidan Wang Yun Zhuang Qun Shen
author_facet	Jiwei Zhong Haidan Wang Yun Zhuang Qun Shen
author_sort	Jiwei Zhong
title	Identification of the antibacterial mechanism of cryptotanshinone on methicillin-resistant Staphylococcus aureus using bioinformatics analysis
title_short	Identification of the antibacterial mechanism of cryptotanshinone on methicillin-resistant Staphylococcus aureus using bioinformatics analysis
title_full	Identification of the antibacterial mechanism of cryptotanshinone on methicillin-resistant Staphylococcus aureus using bioinformatics analysis
title_fullStr	Identification of the antibacterial mechanism of cryptotanshinone on methicillin-resistant Staphylococcus aureus using bioinformatics analysis
title_full_unstemmed	Identification of the antibacterial mechanism of cryptotanshinone on methicillin-resistant Staphylococcus aureus using bioinformatics analysis
title_sort	identification of the antibacterial mechanism of cryptotanshinone on methicillin-resistant staphylococcus aureus using bioinformatics analysis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/987422a4ec194de4b118c71328119011
work_keys_str_mv	AT jiweizhong identificationoftheantibacterialmechanismofcryptotanshinoneonmethicillinresistantstaphylococcusaureususingbioinformaticsanalysis AT haidanwang identificationoftheantibacterialmechanismofcryptotanshinoneonmethicillinresistantstaphylococcusaureususingbioinformaticsanalysis AT yunzhuang identificationoftheantibacterialmechanismofcryptotanshinoneonmethicillinresistantstaphylococcusaureususingbioinformaticsanalysis AT qunshen identificationoftheantibacterialmechanismofcryptotanshinoneonmethicillinresistantstaphylococcusaureususingbioinformaticsanalysis
_version_	1718442548354088960

Identification of the antibacterial mechanism of cryptotanshinone on methicillin-resistant Staphylococcus aureus using bioinformatics analysis

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