Purified Native and Recombinant Major <i>Alternaria alternata</i> Allergen (Alt a 1) Induces Allergic Asthma in the Murine Model
Aeroallergens such us the spores of <i>Alternaria alternata</i> are described as the most important agents associated with respiratory allergies and severe asthma. Various experimental models of asthma have been developed using <i>A. alternata</i> extracts to study the pathog...
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| Autores principales: | , , , , |
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| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
MDPI AG
2021
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| Materias: | |
| Acceso en línea: | https://doaj.org/article/98765fbdd0b34ea5a856b12deb741ce3 |
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| Sumario: | Aeroallergens such us the spores of <i>Alternaria alternata</i> are described as the most important agents associated with respiratory allergies and severe asthma. Various experimental models of asthma have been developed using <i>A. alternata</i> extracts to study the pathogenesis of asthma, establishing the main parameters that trigger the asthmatic response. In this study, we describe a mouse model of asthma induced only by Alt a 1. To induce the allergic response, mice were challenged intranasally with the major allergen of <i>A. alternata</i>, Alt a 1. The presence of eosinophils in the lungs, elevated concentrations of Th2 family cytokines, lymphocyte proliferation and elevated IgE total serum levels indicated that the sensitisation and challenge with Alt a 1 induced the development of airway inflammation. Histological studies showed an eosinophilic cellular infiltrate in the lung tissue of mice instilled with Alt a 1. We demonstrate that Alt a 1 alone is capable of inducing a lung inflammatory response with an increase in IgE serum levels mimicking the allergic asthma immunoresponse when it is administered into BALB/c mice. This model will allow the evaluation of the immunoregulatory or immunotolerant capacity of several molecules that can be used in targeted immunotherapy for fungal allergic asthma. |
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