Purified Native and Recombinant Major Alternaria alternata Allergen (Alt a 1) Induces Allergic Asthma in the Murine Model

Aeroallergens such us the spores of Alternaria alternata are described as the most important agents associated with respiratory allergies and severe asthma. Various experimental models of asthma have been developed using A. alternata extracts to study the pathog...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Ainara Vélez-del-Burgo, Patricia Sánchez, Ester Suñen, Jorge Martínez, Idoia Postigo
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	asthma <i>Alternaria alternata</i> Alt a 1 mice Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/98765fbdd0b34ea5a856b12deb741ce3
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:98765fbdd0b34ea5a856b12deb741ce3
record_format	dspace
spelling	oai:doaj.org-article:98765fbdd0b34ea5a856b12deb741ce32021-11-25T18:05:32ZPurified Native and Recombinant Major <i>Alternaria alternata</i> Allergen (Alt a 1) Induces Allergic Asthma in the Murine Model10.3390/jof71108962309-608Xhttps://doaj.org/article/98765fbdd0b34ea5a856b12deb741ce32021-10-01T00:00:00Zhttps://www.mdpi.com/2309-608X/7/11/896https://doaj.org/toc/2309-608XAeroallergens such us the spores of <i>Alternaria alternata</i> are described as the most important agents associated with respiratory allergies and severe asthma. Various experimental models of asthma have been developed using <i>A. alternata</i> extracts to study the pathogenesis of asthma, establishing the main parameters that trigger the asthmatic response. In this study, we describe a mouse model of asthma induced only by Alt a 1. To induce the allergic response, mice were challenged intranasally with the major allergen of <i>A. alternata</i>, Alt a 1. The presence of eosinophils in the lungs, elevated concentrations of Th2 family cytokines, lymphocyte proliferation and elevated IgE total serum levels indicated that the sensitisation and challenge with Alt a 1 induced the development of airway inflammation. Histological studies showed an eosinophilic cellular infiltrate in the lung tissue of mice instilled with Alt a 1. We demonstrate that Alt a 1 alone is capable of inducing a lung inflammatory response with an increase in IgE serum levels mimicking the allergic asthma immunoresponse when it is administered into BALB/c mice. This model will allow the evaluation of the immunoregulatory or immunotolerant capacity of several molecules that can be used in targeted immunotherapy for fungal allergic asthma.Ainara Vélez-del-BurgoPatricia SánchezEster SuñenJorge MartínezIdoia PostigoMDPI AGarticleasthma<i>Alternaria alternata</i>Alt a 1miceBiology (General)QH301-705.5ENJournal of Fungi, Vol 7, Iss 896, p 896 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	asthma <i>Alternaria alternata</i> Alt a 1 mice Biology (General) QH301-705.5
spellingShingle	asthma <i>Alternaria alternata</i> Alt a 1 mice Biology (General) QH301-705.5 Ainara Vélez-del-Burgo Patricia Sánchez Ester Suñen Jorge Martínez Idoia Postigo Purified Native and Recombinant Major <i>Alternaria alternata</i> Allergen (Alt a 1) Induces Allergic Asthma in the Murine Model
description	Aeroallergens such us the spores of <i>Alternaria alternata</i> are described as the most important agents associated with respiratory allergies and severe asthma. Various experimental models of asthma have been developed using <i>A. alternata</i> extracts to study the pathogenesis of asthma, establishing the main parameters that trigger the asthmatic response. In this study, we describe a mouse model of asthma induced only by Alt a 1. To induce the allergic response, mice were challenged intranasally with the major allergen of <i>A. alternata</i>, Alt a 1. The presence of eosinophils in the lungs, elevated concentrations of Th2 family cytokines, lymphocyte proliferation and elevated IgE total serum levels indicated that the sensitisation and challenge with Alt a 1 induced the development of airway inflammation. Histological studies showed an eosinophilic cellular infiltrate in the lung tissue of mice instilled with Alt a 1. We demonstrate that Alt a 1 alone is capable of inducing a lung inflammatory response with an increase in IgE serum levels mimicking the allergic asthma immunoresponse when it is administered into BALB/c mice. This model will allow the evaluation of the immunoregulatory or immunotolerant capacity of several molecules that can be used in targeted immunotherapy for fungal allergic asthma.
format	article
author	Ainara Vélez-del-Burgo Patricia Sánchez Ester Suñen Jorge Martínez Idoia Postigo
author_facet	Ainara Vélez-del-Burgo Patricia Sánchez Ester Suñen Jorge Martínez Idoia Postigo
author_sort	Ainara Vélez-del-Burgo
title	Purified Native and Recombinant Major <i>Alternaria alternata</i> Allergen (Alt a 1) Induces Allergic Asthma in the Murine Model
title_short	Purified Native and Recombinant Major <i>Alternaria alternata</i> Allergen (Alt a 1) Induces Allergic Asthma in the Murine Model
title_full	Purified Native and Recombinant Major <i>Alternaria alternata</i> Allergen (Alt a 1) Induces Allergic Asthma in the Murine Model
title_fullStr	Purified Native and Recombinant Major <i>Alternaria alternata</i> Allergen (Alt a 1) Induces Allergic Asthma in the Murine Model
title_full_unstemmed	Purified Native and Recombinant Major <i>Alternaria alternata</i> Allergen (Alt a 1) Induces Allergic Asthma in the Murine Model
title_sort	purified native and recombinant major <i>alternaria alternata</i> allergen (alt a 1) induces allergic asthma in the murine model
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/98765fbdd0b34ea5a856b12deb741ce3
work_keys_str_mv	AT ainaravelezdelburgo purifiednativeandrecombinantmajorialternariaalternataiallergenalta1inducesallergicasthmainthemurinemodel AT patriciasanchez purifiednativeandrecombinantmajorialternariaalternataiallergenalta1inducesallergicasthmainthemurinemodel AT estersunen purifiednativeandrecombinantmajorialternariaalternataiallergenalta1inducesallergicasthmainthemurinemodel AT jorgemartinez purifiednativeandrecombinantmajorialternariaalternataiallergenalta1inducesallergicasthmainthemurinemodel AT idoiapostigo purifiednativeandrecombinantmajorialternariaalternataiallergenalta1inducesallergicasthmainthemurinemodel
_version_	1718411631730360320

Purified Native and Recombinant Major <i>Alternaria alternata</i> Allergen (Alt a 1) Induces Allergic Asthma in the Murine Model

Ejemplares similares