Effect of Diquafosol Ophthalmic Solution on Airflow-Induced Ocular Surface Disorder in Diabetic Rats

Atsuyoshi Dota, Asuka Sakamoto, Takashi Nagano, Tadahiro Murakami, Takeshi Matsugi Nara Research & Development Center, Santen Pharmaceutical Co., Ltd, Ikoma-shi, Nara, JapanCorrespondence: Atsuyoshi DotaNara Research & Development Center, Santen Pharmaceutical Co., Ltd., 8916-16 Ikom...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Dota A, Sakamoto A, Nagano T, Murakami T, Matsugi T
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	diquafosol ophthalmic solution corneal epithelial damage tear volume diabetic keratopathy goto–kakizaki rat Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/987742177c7e459d905ced74f754750f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:987742177c7e459d905ced74f754750f
record_format	dspace
spelling	oai:doaj.org-article:987742177c7e459d905ced74f754750f2021-12-02T08:58:22ZEffect of Diquafosol Ophthalmic Solution on Airflow-Induced Ocular Surface Disorder in Diabetic Rats1177-5483https://doaj.org/article/987742177c7e459d905ced74f754750f2020-04-01T00:00:00Zhttps://www.dovepress.com/effect-of-diquafosol-ophthalmic-solution-on-airflow-induced-ocular-sur-peer-reviewed-article-OPTHhttps://doaj.org/toc/1177-5483Atsuyoshi Dota, Asuka Sakamoto, Takashi Nagano, Tadahiro Murakami, Takeshi Matsugi Nara Research & Development Center, Santen Pharmaceutical Co., Ltd, Ikoma-shi, Nara, JapanCorrespondence: Atsuyoshi DotaNara Research & Development Center, Santen Pharmaceutical Co., Ltd., 8916-16 Ikoma-shi, Nara 630-0101, JapanTel +81 743 79 4525Fax +81 743 79 4591Email atsuyoshi.dota@santen.comPurpose: To examine the effect of 3% diquafosol ophthalmic solution (DQS) on ocular surface disorders in diabetic model rats maintained in a continuous airflow condition.Methods: Goto–Kakizaki (GK) rats, a spontaneous model of type 2 diabetes, were exposed to constant airflow for 8 weeks. After the establishment of the animal model in this environment, DQS or saline was instilled six times a day into GK rat eyes for 6 weeks. Schirmer’s test was performed before and after 6-week instillations. Corneal fluorescein staining was scored at 2-, 4-, and 6-week instillations. Touch thresholds for the cornea were also determined using a Cochet–Bonnet esthesiometer before and after 6-week instillations.Results: The mean Schirmer’s test score after instillation of DQS was twice higher than that recorded for saline alone. DQS significantly decreased corneal staining scores at 4- and 6-week instillations. No changes in touch thresholds were observed before and after 6-week instillations.Conclusion: These results suggest that DQS improves corneal epithelial damage by stimulating tear secretion without influencing corneal sensation in diabetic keratopathy. Thus, DQS may have potential for treatment of diabetic patients with dry eye.Keywords: diquafosol ophthalmic solution, corneal epithelial damage, tear volume, diabetic keratopathy, Goto–Kakizaki ratDota ASakamoto ANagano TMurakami TMatsugi TDove Medical Pressarticlediquafosol ophthalmic solutioncorneal epithelial damagetear volumediabetic keratopathygoto–kakizaki ratOphthalmologyRE1-994ENClinical Ophthalmology, Vol Volume 14, Pp 1019-1024 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	diquafosol ophthalmic solution corneal epithelial damage tear volume diabetic keratopathy goto–kakizaki rat Ophthalmology RE1-994
spellingShingle	diquafosol ophthalmic solution corneal epithelial damage tear volume diabetic keratopathy goto–kakizaki rat Ophthalmology RE1-994 Dota A Sakamoto A Nagano T Murakami T Matsugi T Effect of Diquafosol Ophthalmic Solution on Airflow-Induced Ocular Surface Disorder in Diabetic Rats
description	Atsuyoshi Dota, Asuka Sakamoto, Takashi Nagano, Tadahiro Murakami, Takeshi Matsugi Nara Research & Development Center, Santen Pharmaceutical Co., Ltd, Ikoma-shi, Nara, JapanCorrespondence: Atsuyoshi DotaNara Research & Development Center, Santen Pharmaceutical Co., Ltd., 8916-16 Ikoma-shi, Nara 630-0101, JapanTel +81 743 79 4525Fax +81 743 79 4591Email atsuyoshi.dota@santen.comPurpose: To examine the effect of 3% diquafosol ophthalmic solution (DQS) on ocular surface disorders in diabetic model rats maintained in a continuous airflow condition.Methods: Goto–Kakizaki (GK) rats, a spontaneous model of type 2 diabetes, were exposed to constant airflow for 8 weeks. After the establishment of the animal model in this environment, DQS or saline was instilled six times a day into GK rat eyes for 6 weeks. Schirmer’s test was performed before and after 6-week instillations. Corneal fluorescein staining was scored at 2-, 4-, and 6-week instillations. Touch thresholds for the cornea were also determined using a Cochet–Bonnet esthesiometer before and after 6-week instillations.Results: The mean Schirmer’s test score after instillation of DQS was twice higher than that recorded for saline alone. DQS significantly decreased corneal staining scores at 4- and 6-week instillations. No changes in touch thresholds were observed before and after 6-week instillations.Conclusion: These results suggest that DQS improves corneal epithelial damage by stimulating tear secretion without influencing corneal sensation in diabetic keratopathy. Thus, DQS may have potential for treatment of diabetic patients with dry eye.Keywords: diquafosol ophthalmic solution, corneal epithelial damage, tear volume, diabetic keratopathy, Goto–Kakizaki rat
format	article
author	Dota A Sakamoto A Nagano T Murakami T Matsugi T
author_facet	Dota A Sakamoto A Nagano T Murakami T Matsugi T
author_sort	Dota A
title	Effect of Diquafosol Ophthalmic Solution on Airflow-Induced Ocular Surface Disorder in Diabetic Rats
title_short	Effect of Diquafosol Ophthalmic Solution on Airflow-Induced Ocular Surface Disorder in Diabetic Rats
title_full	Effect of Diquafosol Ophthalmic Solution on Airflow-Induced Ocular Surface Disorder in Diabetic Rats
title_fullStr	Effect of Diquafosol Ophthalmic Solution on Airflow-Induced Ocular Surface Disorder in Diabetic Rats
title_full_unstemmed	Effect of Diquafosol Ophthalmic Solution on Airflow-Induced Ocular Surface Disorder in Diabetic Rats
title_sort	effect of diquafosol ophthalmic solution on airflow-induced ocular surface disorder in diabetic rats
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/987742177c7e459d905ced74f754750f
work_keys_str_mv	AT dotaa effectofdiquafosolophthalmicsolutiononairflowinducedocularsurfacedisorderindiabeticrats AT sakamotoa effectofdiquafosolophthalmicsolutiononairflowinducedocularsurfacedisorderindiabeticrats AT naganot effectofdiquafosolophthalmicsolutiononairflowinducedocularsurfacedisorderindiabeticrats AT murakamit effectofdiquafosolophthalmicsolutiononairflowinducedocularsurfacedisorderindiabeticrats AT matsugit effectofdiquafosolophthalmicsolutiononairflowinducedocularsurfacedisorderindiabeticrats
_version_	1718398318745223168

Effect of Diquafosol Ophthalmic Solution on Airflow-Induced Ocular Surface Disorder in Diabetic Rats

Ejemplares similares