MiR-21, miR-34a, miR-125b, miR-181d and miR-648 levels inversely correlate with MGMT and TP53 expression in primary glioblastoma patients
Introduction TP53 and MGMT alterations play a crucial role in glioblastoma (GB) pathogenesis. TP53 and MGMT function is affected by several pathologic mechanisms, such as point mutations or promoter methylation, which are well characterized. Expression of both genes can be regulated by other mechani...
Guardado en:
| Autores principales: | , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
Termedia Publishing House
2019
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/9878f2c77af748089cded2e35d56be05 |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:9878f2c77af748089cded2e35d56be05 |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:9878f2c77af748089cded2e35d56be052021-12-02T19:15:42ZMiR-21, miR-34a, miR-125b, miR-181d and miR-648 levels inversely correlate with MGMT and TP53 expression in primary glioblastoma patients1734-19221896-915110.5114/aoms.2017.69374https://doaj.org/article/9878f2c77af748089cded2e35d56be052019-03-01T00:00:00Zhttps://www.archivesofmedicalscience.com/MiR-21-miR-34a-miR-125b-miR-181d-and-miR-648-levels-inversely-correlate-with-MGMT,69743,0,2.htmlhttps://doaj.org/toc/1734-1922https://doaj.org/toc/1896-9151Introduction TP53 and MGMT alterations play a crucial role in glioblastoma (GB) pathogenesis. TP53 and MGMT function is affected by several pathologic mechanisms, such as point mutations or promoter methylation, which are well characterized. Expression of both genes can be regulated by other mechanisms as well, e.g., microRNAs (miRNAs). Moreover, cross-talk among various pathologic processes may occur, further affecting MGMT and TP53 functionality. Material and methods In 49 GB patients, we analyzed the possible associations between TP53 and its miRNA regulators miR-125b, miR-21, and miR-34a, as well as MGMT and its miRNA regulators miR-181d and miR-648. We evaluated the possible influence of mutational and methylation status on the pre-identified associations. Results In patients with immunohistochemistry-detected TP53 overexpression, expression levels of miR-34a and TP53 were negatively correlated (r = –0.56, p = 0.0195), and in patients with TP53 mutations, expression levels of TP53 and miR-21 were negatively correlated (r = –0.67, p = 0.0330). In patients with MGMT methylation, expression levels of MGMT were negatively correlated with miR-648 and miR-125b expression levels (r = –0.61, p = 0.0269 and r = –0.34, p = 0.0727, respectively). Conclusions Our findings demonstrate that selected miRNAs are significantly correlated with MGMT and TP53 levels, but the extent of this correlation differs regarding the TP53 and MGMT mutational and promoter methylation status.Dorota Jesionek-KupnickaMarcin BraunBerenika Trąbska-KluchJoanna CzechMałgorzata SzybkaBożena SzymańskaDominika Kulczycka-WojdalaMichał BieńkowskiRadzisław KordekIzabela ZawlikTermedia Publishing Housearticlemicrornamgmttp53o6-methylguanine-dna methyltransferaseglioblastoma multiforme (gbm)gbMedicineRENArchives of Medical Science, Vol 15, Iss 2, Pp 504-512 (2019) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
microrna mgmt tp53 o6-methylguanine-dna methyltransferase glioblastoma multiforme (gbm) gb Medicine R |
| spellingShingle |
microrna mgmt tp53 o6-methylguanine-dna methyltransferase glioblastoma multiforme (gbm) gb Medicine R Dorota Jesionek-Kupnicka Marcin Braun Berenika Trąbska-Kluch Joanna Czech Małgorzata Szybka Bożena Szymańska Dominika Kulczycka-Wojdala Michał Bieńkowski Radzisław Kordek Izabela Zawlik MiR-21, miR-34a, miR-125b, miR-181d and miR-648 levels inversely correlate with MGMT and TP53 expression in primary glioblastoma patients |
| description |
Introduction
TP53 and MGMT alterations play a crucial role in glioblastoma (GB) pathogenesis. TP53 and MGMT function is affected by several pathologic mechanisms, such as point mutations or promoter methylation, which are well characterized. Expression of both genes can be regulated by other mechanisms as well, e.g., microRNAs (miRNAs). Moreover, cross-talk among various pathologic processes may occur, further affecting MGMT and TP53 functionality.
Material and methods
In 49 GB patients, we analyzed the possible associations between TP53 and its miRNA regulators miR-125b, miR-21, and miR-34a, as well as MGMT and its miRNA regulators miR-181d and miR-648. We evaluated the possible influence of mutational and methylation status on the pre-identified associations.
Results
In patients with immunohistochemistry-detected TP53 overexpression, expression levels of miR-34a and TP53 were negatively correlated (r = –0.56, p = 0.0195), and in patients with TP53 mutations, expression levels of TP53 and miR-21 were negatively correlated (r = –0.67, p = 0.0330). In patients with MGMT methylation, expression levels of MGMT were negatively correlated with miR-648 and miR-125b expression levels (r = –0.61, p = 0.0269 and r = –0.34, p = 0.0727, respectively).
Conclusions
Our findings demonstrate that selected miRNAs are significantly correlated with MGMT and TP53 levels, but the extent of this correlation differs regarding the TP53 and MGMT mutational and promoter methylation status. |
| format |
article |
| author |
Dorota Jesionek-Kupnicka Marcin Braun Berenika Trąbska-Kluch Joanna Czech Małgorzata Szybka Bożena Szymańska Dominika Kulczycka-Wojdala Michał Bieńkowski Radzisław Kordek Izabela Zawlik |
| author_facet |
Dorota Jesionek-Kupnicka Marcin Braun Berenika Trąbska-Kluch Joanna Czech Małgorzata Szybka Bożena Szymańska Dominika Kulczycka-Wojdala Michał Bieńkowski Radzisław Kordek Izabela Zawlik |
| author_sort |
Dorota Jesionek-Kupnicka |
| title |
MiR-21, miR-34a, miR-125b, miR-181d and miR-648 levels inversely correlate with MGMT and TP53 expression in primary glioblastoma patients |
| title_short |
MiR-21, miR-34a, miR-125b, miR-181d and miR-648 levels inversely correlate with MGMT and TP53 expression in primary glioblastoma patients |
| title_full |
MiR-21, miR-34a, miR-125b, miR-181d and miR-648 levels inversely correlate with MGMT and TP53 expression in primary glioblastoma patients |
| title_fullStr |
MiR-21, miR-34a, miR-125b, miR-181d and miR-648 levels inversely correlate with MGMT and TP53 expression in primary glioblastoma patients |
| title_full_unstemmed |
MiR-21, miR-34a, miR-125b, miR-181d and miR-648 levels inversely correlate with MGMT and TP53 expression in primary glioblastoma patients |
| title_sort |
mir-21, mir-34a, mir-125b, mir-181d and mir-648 levels inversely correlate with mgmt and tp53 expression in primary glioblastoma patients |
| publisher |
Termedia Publishing House |
| publishDate |
2019 |
| url |
https://doaj.org/article/9878f2c77af748089cded2e35d56be05 |
| work_keys_str_mv |
AT dorotajesionekkupnicka mir21mir34amir125bmir181dandmir648levelsinverselycorrelatewithmgmtandtp53expressioninprimaryglioblastomapatients AT marcinbraun mir21mir34amir125bmir181dandmir648levelsinverselycorrelatewithmgmtandtp53expressioninprimaryglioblastomapatients AT berenikatrabskakluch mir21mir34amir125bmir181dandmir648levelsinverselycorrelatewithmgmtandtp53expressioninprimaryglioblastomapatients AT joannaczech mir21mir34amir125bmir181dandmir648levelsinverselycorrelatewithmgmtandtp53expressioninprimaryglioblastomapatients AT małgorzataszybka mir21mir34amir125bmir181dandmir648levelsinverselycorrelatewithmgmtandtp53expressioninprimaryglioblastomapatients AT bozenaszymanska mir21mir34amir125bmir181dandmir648levelsinverselycorrelatewithmgmtandtp53expressioninprimaryglioblastomapatients AT dominikakulczyckawojdala mir21mir34amir125bmir181dandmir648levelsinverselycorrelatewithmgmtandtp53expressioninprimaryglioblastomapatients AT michałbienkowski mir21mir34amir125bmir181dandmir648levelsinverselycorrelatewithmgmtandtp53expressioninprimaryglioblastomapatients AT radzisławkordek mir21mir34amir125bmir181dandmir648levelsinverselycorrelatewithmgmtandtp53expressioninprimaryglioblastomapatients AT izabelazawlik mir21mir34amir125bmir181dandmir648levelsinverselycorrelatewithmgmtandtp53expressioninprimaryglioblastomapatients |
| _version_ |
1718376987301511168 |