A novel virulence strategy for Pseudomonas aeruginosa mediated by an autotransporter with arginine-specific aminopeptidase activity.

The opportunistic human pathogen, Pseudomonas aeruginosa, is a major cause of infections in chronic wounds, burns and the lungs of cystic fibrosis patients. The P. aeruginosa genome encodes at least three proteins exhibiting the characteristic three domain structure of autotransporters, but much rem...

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Autores principales:	Jeni C A Luckett, Owen Darch, Chase Watters, Manal Abuoun, Victoria Wright, Esteban Paredes-Osses, Jenny Ward, Hana Goto, Stephan Heeb, Stéphanie Pommier, Kendra P Rumbaugh, Miguel Cámara, Kim R Hardie
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/987c15aed96e48fea9b51576b92aee63
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spelling	oai:doaj.org-article:987c15aed96e48fea9b51576b92aee632021-11-18T06:04:03ZA novel virulence strategy for Pseudomonas aeruginosa mediated by an autotransporter with arginine-specific aminopeptidase activity.1553-73661553-737410.1371/journal.ppat.1002854https://doaj.org/article/987c15aed96e48fea9b51576b92aee632012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22927813/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374The opportunistic human pathogen, Pseudomonas aeruginosa, is a major cause of infections in chronic wounds, burns and the lungs of cystic fibrosis patients. The P. aeruginosa genome encodes at least three proteins exhibiting the characteristic three domain structure of autotransporters, but much remains to be understood about the functions of these three proteins and their role in pathogenicity. Autotransporters are the largest family of secreted proteins in Gram-negative bacteria, and those characterised are virulence factors. Here, we demonstrate that the PA0328 autotransporter is a cell-surface tethered, arginine-specific aminopeptidase, and have defined its active site by site directed mutagenesis. Hence, we have assigned PA0328 with the name AaaA, for arginine-specific autotransporter of P. aeruginosa. We show that AaaA provides a fitness advantage in environments where the sole source of nitrogen is peptides with an aminoterminal arginine, and that this could be important for establishing an infection, as the lack of AaaA led to attenuation in a mouse chronic wound infection which correlated with lower levels of the cytokines TNFα, IL-1α, KC and COX-2. Consequently AaaA is an important virulence factor playing a significant role in the successful establishment of P. aeruginosa infections.Jeni C A LuckettOwen DarchChase WattersManal AbuounVictoria WrightEsteban Paredes-OssesJenny WardHana GotoStephan HeebStéphanie PommierKendra P RumbaughMiguel CámaraKim R HardiePublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 8, Iss 8, p e1002854 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Jeni C A Luckett Owen Darch Chase Watters Manal Abuoun Victoria Wright Esteban Paredes-Osses Jenny Ward Hana Goto Stephan Heeb Stéphanie Pommier Kendra P Rumbaugh Miguel Cámara Kim R Hardie A novel virulence strategy for Pseudomonas aeruginosa mediated by an autotransporter with arginine-specific aminopeptidase activity.
description	The opportunistic human pathogen, Pseudomonas aeruginosa, is a major cause of infections in chronic wounds, burns and the lungs of cystic fibrosis patients. The P. aeruginosa genome encodes at least three proteins exhibiting the characteristic three domain structure of autotransporters, but much remains to be understood about the functions of these three proteins and their role in pathogenicity. Autotransporters are the largest family of secreted proteins in Gram-negative bacteria, and those characterised are virulence factors. Here, we demonstrate that the PA0328 autotransporter is a cell-surface tethered, arginine-specific aminopeptidase, and have defined its active site by site directed mutagenesis. Hence, we have assigned PA0328 with the name AaaA, for arginine-specific autotransporter of P. aeruginosa. We show that AaaA provides a fitness advantage in environments where the sole source of nitrogen is peptides with an aminoterminal arginine, and that this could be important for establishing an infection, as the lack of AaaA led to attenuation in a mouse chronic wound infection which correlated with lower levels of the cytokines TNFα, IL-1α, KC and COX-2. Consequently AaaA is an important virulence factor playing a significant role in the successful establishment of P. aeruginosa infections.
format	article
author	Jeni C A Luckett Owen Darch Chase Watters Manal Abuoun Victoria Wright Esteban Paredes-Osses Jenny Ward Hana Goto Stephan Heeb Stéphanie Pommier Kendra P Rumbaugh Miguel Cámara Kim R Hardie
author_facet	Jeni C A Luckett Owen Darch Chase Watters Manal Abuoun Victoria Wright Esteban Paredes-Osses Jenny Ward Hana Goto Stephan Heeb Stéphanie Pommier Kendra P Rumbaugh Miguel Cámara Kim R Hardie
author_sort	Jeni C A Luckett
title	A novel virulence strategy for Pseudomonas aeruginosa mediated by an autotransporter with arginine-specific aminopeptidase activity.
title_short	A novel virulence strategy for Pseudomonas aeruginosa mediated by an autotransporter with arginine-specific aminopeptidase activity.
title_full	A novel virulence strategy for Pseudomonas aeruginosa mediated by an autotransporter with arginine-specific aminopeptidase activity.
title_fullStr	A novel virulence strategy for Pseudomonas aeruginosa mediated by an autotransporter with arginine-specific aminopeptidase activity.
title_full_unstemmed	A novel virulence strategy for Pseudomonas aeruginosa mediated by an autotransporter with arginine-specific aminopeptidase activity.
title_sort	novel virulence strategy for pseudomonas aeruginosa mediated by an autotransporter with arginine-specific aminopeptidase activity.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/987c15aed96e48fea9b51576b92aee63
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A novel virulence strategy for Pseudomonas aeruginosa mediated by an autotransporter with arginine-specific aminopeptidase activity.

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