EFFICIENCY EVALUATION OF THE HOME-PRODUCED RECOMBINANT HUMAN CYTOKINES FROM the sci-store.ru FOR GENERATION OF MONOCYTE-DERIVED DENDRITIC CELLS

Dendritic cells are specialized antigen-presenting cells which are required to involve naive T-lymphocytes into immune response. Dendritic cells are used in numerous research studies, and have also found practical application as a main component of dendritic cell vaccines. In vitro induction of thei...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	V. Yu. Talayev, M. V. Talayeva, E. V. Voronina, I. E. Zaichenko
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2018
Materias:	dendritic cells monocytes cytokines maturation membrane molecules Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/98892bb5886a48178c02f205816709b7
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:98892bb5886a48178c02f205816709b7
record_format	dspace
spelling	oai:doaj.org-article:98892bb5886a48178c02f205816709b72021-11-18T08:03:47ZEFFICIENCY EVALUATION OF THE HOME-PRODUCED RECOMBINANT HUMAN CYTOKINES FROM the sci-store.ru FOR GENERATION OF MONOCYTE-DERIVED DENDRITIC CELLS1563-06252313-741X10.15789/1563-0625-2018-4-535-542https://doaj.org/article/98892bb5886a48178c02f205816709b72018-06-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1561https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XDendritic cells are specialized antigen-presenting cells which are required to involve naive T-lymphocytes into immune response. Dendritic cells are used in numerous research studies, and have also found practical application as a main component of dendritic cell vaccines. In vitro induction of their maturation from monocytes using cytokines is the most widely used method to obtain these cells. In present study, we compared phenotypes of both immature and mature dendritic cells induced from monocytes of adult healthy donors by means of recombinant cytokines provided by different manufacturers. It is shown that recombinant human IL-4 and GM-CSF supplied by sci-store.ru could effectively stimulate in vitro differentiation of human monocytes to immature monocytic dendritic cells. The resulting immature dendritic cells exhibited a typical morphology and phenotype characterized by the absence of CD14 monocyte marker as well as HLA-DR and CD80 costimulatory molecule expression. A fraction of these cells expressed the CD86 costimulatory molecule. The recombinant human IL-6 from sci-store.ru, when used in a mixture of inflammatory mediators (IL-6, IL-1β, TNFα and PGE2), has induced effective terminal differentiation of immature dendritic cells to mature dendritic cells exhibiting typical morphology and phenotype. Virtually all dendritic cells obtained with this reagent were devoid of CD14, and expressed HLA-DR, CD80, CD83 and CD86 at high fluorescence intensity.V. Yu. TalayevM. V. TalayevaE. V. VoroninaI. E. ZaichenkoSPb RAACIarticledendritic cellsmonocytescytokinesmaturationmembrane moleculesImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 20, Iss 4, Pp 535-542 (2018)
institution	DOAJ
collection	DOAJ
language	RU
topic	dendritic cells monocytes cytokines maturation membrane molecules Immunologic diseases. Allergy RC581-607
spellingShingle	dendritic cells monocytes cytokines maturation membrane molecules Immunologic diseases. Allergy RC581-607 V. Yu. Talayev M. V. Talayeva E. V. Voronina I. E. Zaichenko EFFICIENCY EVALUATION OF THE HOME-PRODUCED RECOMBINANT HUMAN CYTOKINES FROM the sci-store.ru FOR GENERATION OF MONOCYTE-DERIVED DENDRITIC CELLS
description	Dendritic cells are specialized antigen-presenting cells which are required to involve naive T-lymphocytes into immune response. Dendritic cells are used in numerous research studies, and have also found practical application as a main component of dendritic cell vaccines. In vitro induction of their maturation from monocytes using cytokines is the most widely used method to obtain these cells. In present study, we compared phenotypes of both immature and mature dendritic cells induced from monocytes of adult healthy donors by means of recombinant cytokines provided by different manufacturers. It is shown that recombinant human IL-4 and GM-CSF supplied by sci-store.ru could effectively stimulate in vitro differentiation of human monocytes to immature monocytic dendritic cells. The resulting immature dendritic cells exhibited a typical morphology and phenotype characterized by the absence of CD14 monocyte marker as well as HLA-DR and CD80 costimulatory molecule expression. A fraction of these cells expressed the CD86 costimulatory molecule. The recombinant human IL-6 from sci-store.ru, when used in a mixture of inflammatory mediators (IL-6, IL-1β, TNFα and PGE2), has induced effective terminal differentiation of immature dendritic cells to mature dendritic cells exhibiting typical morphology and phenotype. Virtually all dendritic cells obtained with this reagent were devoid of CD14, and expressed HLA-DR, CD80, CD83 and CD86 at high fluorescence intensity.
format	article
author	V. Yu. Talayev M. V. Talayeva E. V. Voronina I. E. Zaichenko
author_facet	V. Yu. Talayev M. V. Talayeva E. V. Voronina I. E. Zaichenko
author_sort	V. Yu. Talayev
title	EFFICIENCY EVALUATION OF THE HOME-PRODUCED RECOMBINANT HUMAN CYTOKINES FROM the sci-store.ru FOR GENERATION OF MONOCYTE-DERIVED DENDRITIC CELLS
title_short	EFFICIENCY EVALUATION OF THE HOME-PRODUCED RECOMBINANT HUMAN CYTOKINES FROM the sci-store.ru FOR GENERATION OF MONOCYTE-DERIVED DENDRITIC CELLS
title_full	EFFICIENCY EVALUATION OF THE HOME-PRODUCED RECOMBINANT HUMAN CYTOKINES FROM the sci-store.ru FOR GENERATION OF MONOCYTE-DERIVED DENDRITIC CELLS
title_fullStr	EFFICIENCY EVALUATION OF THE HOME-PRODUCED RECOMBINANT HUMAN CYTOKINES FROM the sci-store.ru FOR GENERATION OF MONOCYTE-DERIVED DENDRITIC CELLS
title_full_unstemmed	EFFICIENCY EVALUATION OF THE HOME-PRODUCED RECOMBINANT HUMAN CYTOKINES FROM the sci-store.ru FOR GENERATION OF MONOCYTE-DERIVED DENDRITIC CELLS
title_sort	efficiency evaluation of the home-produced recombinant human cytokines from the sci-store.ru for generation of monocyte-derived dendritic cells
publisher	SPb RAACI
publishDate	2018
url	https://doaj.org/article/98892bb5886a48178c02f205816709b7
work_keys_str_mv	AT vyutalayev efficiencyevaluationofthehomeproducedrecombinanthumancytokinesfromthescistoreruforgenerationofmonocytederiveddendriticcells AT mvtalayeva efficiencyevaluationofthehomeproducedrecombinanthumancytokinesfromthescistoreruforgenerationofmonocytederiveddendriticcells AT evvoronina efficiencyevaluationofthehomeproducedrecombinanthumancytokinesfromthescistoreruforgenerationofmonocytederiveddendriticcells AT iezaichenko efficiencyevaluationofthehomeproducedrecombinanthumancytokinesfromthescistoreruforgenerationofmonocytederiveddendriticcells
_version_	1718422368231096320

EFFICIENCY EVALUATION OF THE HOME-PRODUCED RECOMBINANT HUMAN CYTOKINES FROM the sci-store.ru FOR GENERATION OF MONOCYTE-DERIVED DENDRITIC CELLS

Ejemplares similares