Untargeted analysis of the serum metabolome in cats with exocrine pancreatic insufficiency.

Exocrine pancreatic insufficiency (EPI) causes chronic digestive dysfunction in cats, but its pathogenesis and pathophysiology are poorly understood. Untargeted metabolomics is a promising analytic methodology that can reveal novel metabolic features and biomarkers of clinical disease syndromes. The...

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Autores principales:	Patrick C Barko, David A Williams
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/988f9628fae1412fbdd8beaf037780b6
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spelling	oai:doaj.org-article:988f9628fae1412fbdd8beaf037780b62021-12-02T20:13:53ZUntargeted analysis of the serum metabolome in cats with exocrine pancreatic insufficiency.1932-620310.1371/journal.pone.0257856https://doaj.org/article/988f9628fae1412fbdd8beaf037780b62021-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0257856https://doaj.org/toc/1932-6203Exocrine pancreatic insufficiency (EPI) causes chronic digestive dysfunction in cats, but its pathogenesis and pathophysiology are poorly understood. Untargeted metabolomics is a promising analytic methodology that can reveal novel metabolic features and biomarkers of clinical disease syndromes. The purpose of this preliminary study was to use untargeted analysis of the serum metabolome to discover novel aspects of the pathobiology of EPI in cats. Serum samples were collected from 5 cats with EPI and 8 healthy controls. The diagnosis of EPI was confirmed by measurement of subnormal serum feline trypsin-like immunoreactivity (fTLI). Untargeted quantification of serum metabolite utilized ultra-high-performance liquid chromatography-tandem mass spectroscopy. Cats with EPI had significantly increased serum quantities of long-chain fatty acids, polyunsaturated fatty acids, mevalonate pathway intermediates, and endocannabinoids compared with healthy controls. Diacylglycerols, phosphatidylethanolamines, amino acid derivatives, and microbial metabolites were significantly decreased in cats with EPI compared to healthy controls. Diacyclglycerols and amino acid metabolites were positively correlated, and sphingolipids and long-chain fatty acids were negatively correlated with serum fTLI, respectively. These results suggest that EPI in cats is associated with increased lipolysis of peripheral adipose stores, dysfunction of the mevalonate pathway, and altered amino acid metabolism. Differences in microbial metabolites indicate that feline EPI is also associated with enteric microbial dysbiosis. Targeted studies of the metabolome of cats with EPI are warranted to further elucidate the mechanisms of these metabolic derangements and their influence on the pathogenesis and pathophysiology of EPI in cats.Patrick C BarkoDavid A WilliamsPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 16, Iss 9, p e0257856 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Patrick C Barko David A Williams Untargeted analysis of the serum metabolome in cats with exocrine pancreatic insufficiency.
description	Exocrine pancreatic insufficiency (EPI) causes chronic digestive dysfunction in cats, but its pathogenesis and pathophysiology are poorly understood. Untargeted metabolomics is a promising analytic methodology that can reveal novel metabolic features and biomarkers of clinical disease syndromes. The purpose of this preliminary study was to use untargeted analysis of the serum metabolome to discover novel aspects of the pathobiology of EPI in cats. Serum samples were collected from 5 cats with EPI and 8 healthy controls. The diagnosis of EPI was confirmed by measurement of subnormal serum feline trypsin-like immunoreactivity (fTLI). Untargeted quantification of serum metabolite utilized ultra-high-performance liquid chromatography-tandem mass spectroscopy. Cats with EPI had significantly increased serum quantities of long-chain fatty acids, polyunsaturated fatty acids, mevalonate pathway intermediates, and endocannabinoids compared with healthy controls. Diacylglycerols, phosphatidylethanolamines, amino acid derivatives, and microbial metabolites were significantly decreased in cats with EPI compared to healthy controls. Diacyclglycerols and amino acid metabolites were positively correlated, and sphingolipids and long-chain fatty acids were negatively correlated with serum fTLI, respectively. These results suggest that EPI in cats is associated with increased lipolysis of peripheral adipose stores, dysfunction of the mevalonate pathway, and altered amino acid metabolism. Differences in microbial metabolites indicate that feline EPI is also associated with enteric microbial dysbiosis. Targeted studies of the metabolome of cats with EPI are warranted to further elucidate the mechanisms of these metabolic derangements and their influence on the pathogenesis and pathophysiology of EPI in cats.
format	article
author	Patrick C Barko David A Williams
author_facet	Patrick C Barko David A Williams
author_sort	Patrick C Barko
title	Untargeted analysis of the serum metabolome in cats with exocrine pancreatic insufficiency.
title_short	Untargeted analysis of the serum metabolome in cats with exocrine pancreatic insufficiency.
title_full	Untargeted analysis of the serum metabolome in cats with exocrine pancreatic insufficiency.
title_fullStr	Untargeted analysis of the serum metabolome in cats with exocrine pancreatic insufficiency.
title_full_unstemmed	Untargeted analysis of the serum metabolome in cats with exocrine pancreatic insufficiency.
title_sort	untargeted analysis of the serum metabolome in cats with exocrine pancreatic insufficiency.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/988f9628fae1412fbdd8beaf037780b6
work_keys_str_mv	AT patrickcbarko untargetedanalysisoftheserummetabolomeincatswithexocrinepancreaticinsufficiency AT davidawilliams untargetedanalysisoftheserummetabolomeincatswithexocrinepancreaticinsufficiency
_version_	1718374722151907328

Untargeted analysis of the serum metabolome in cats with exocrine pancreatic insufficiency.

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