Evidence for sub-haplogroup h5 of mitochondrial DNA as a risk factor for late onset Alzheimer's disease.
<h4>Background</h4>Alzheimer's Disease (AD) is the most common neurodegenerative disease and the leading cause of dementia among senile subjects. It has been proposed that AD can be caused by defects in mitochondrial oxidative phosphorylation. Given the fundamental contribution of t...
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oai:doaj.org-article:9894e1651792487a9699a941862fe2c62021-11-18T06:36:21ZEvidence for sub-haplogroup h5 of mitochondrial DNA as a risk factor for late onset Alzheimer's disease.1932-620310.1371/journal.pone.0012037https://doaj.org/article/9894e1651792487a9699a941862fe2c62010-08-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20700462/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Alzheimer's Disease (AD) is the most common neurodegenerative disease and the leading cause of dementia among senile subjects. It has been proposed that AD can be caused by defects in mitochondrial oxidative phosphorylation. Given the fundamental contribution of the mitochondrial genome (mtDNA) for the respiratory chain, there have been a number of studies investigating the association between mtDNA inherited variants and multifactorial diseases, however no general consensus has been reached yet on the correlation between mtDNA haplogroups and AD.<h4>Methodology/principal findings</h4>We applied for the first time a high resolution analysis (sequencing of displacement loop and restriction analysis of specific markers in the coding region of mtDNA) to investigate the possible association between mtDNA-inherited sequence variation and AD in 936 AD patients and 776 cognitively assessed normal controls from central and northern Italy. Among over 40 mtDNA sub-haplogroups analysed, we found that sub-haplogroup H5 is a risk factor for AD (OR=1.85, 95% CI:1.04-3.23) in particular for females (OR=2.19, 95% CI:1.06-4.51) and independently from the APOE genotype. Multivariate logistic regression revealed an interaction between H5 and age. When the whole sample is considered, the H5a subgroup of molecules, harboring the 4336 transition in the tRNAGln gene, already associated to AD in early studies, was about threefold more represented in AD patients than in controls (2.0% vs 0.8%; p=0.031), and it might account for the increased frequency of H5 in AD patients (4.2% vs 2.3%). The complete re-sequencing of the 56 mtDNAs belonging to H5 revealed that AD patients showed a trend towards a higher number (p=0.052) of sporadic mutations in tRNA and rRNA genes when compared with controls.<h4>Conclusions</h4>Our results indicate that high resolution analysis of inherited mtDNA sequence variation can help in identifying both ancient polymorphisms defining sub-haplogroups and the accumulation of sporadic mutations associated with complex traits such as AD.Aurelia SantoroValentina BalbiElisa BalducciChiara PirazziniFrancesca RosiniFrancesca TavanoAlessandro AchilliPaola SivieroNadia MinicuciElena BellavistaMichele MishtoStefano SalvioliFrancesca MarchegianiMaurizio CardelliFabiola OlivieriBenedetta NacmiasAndrea Maria ChiamentiLuisa BenussiRoberta GhidoniGiuseppina RoseCarlo GabelliGiuliano BinettiSandro SorbiGaetano CrepaldiGiuseppe PassarinoAntonio TorroniClaudio FranceschiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 8, p e12037 (2010) |
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Medicine R Science Q Aurelia Santoro Valentina Balbi Elisa Balducci Chiara Pirazzini Francesca Rosini Francesca Tavano Alessandro Achilli Paola Siviero Nadia Minicuci Elena Bellavista Michele Mishto Stefano Salvioli Francesca Marchegiani Maurizio Cardelli Fabiola Olivieri Benedetta Nacmias Andrea Maria Chiamenti Luisa Benussi Roberta Ghidoni Giuseppina Rose Carlo Gabelli Giuliano Binetti Sandro Sorbi Gaetano Crepaldi Giuseppe Passarino Antonio Torroni Claudio Franceschi Evidence for sub-haplogroup h5 of mitochondrial DNA as a risk factor for late onset Alzheimer's disease. |
| description |
<h4>Background</h4>Alzheimer's Disease (AD) is the most common neurodegenerative disease and the leading cause of dementia among senile subjects. It has been proposed that AD can be caused by defects in mitochondrial oxidative phosphorylation. Given the fundamental contribution of the mitochondrial genome (mtDNA) for the respiratory chain, there have been a number of studies investigating the association between mtDNA inherited variants and multifactorial diseases, however no general consensus has been reached yet on the correlation between mtDNA haplogroups and AD.<h4>Methodology/principal findings</h4>We applied for the first time a high resolution analysis (sequencing of displacement loop and restriction analysis of specific markers in the coding region of mtDNA) to investigate the possible association between mtDNA-inherited sequence variation and AD in 936 AD patients and 776 cognitively assessed normal controls from central and northern Italy. Among over 40 mtDNA sub-haplogroups analysed, we found that sub-haplogroup H5 is a risk factor for AD (OR=1.85, 95% CI:1.04-3.23) in particular for females (OR=2.19, 95% CI:1.06-4.51) and independently from the APOE genotype. Multivariate logistic regression revealed an interaction between H5 and age. When the whole sample is considered, the H5a subgroup of molecules, harboring the 4336 transition in the tRNAGln gene, already associated to AD in early studies, was about threefold more represented in AD patients than in controls (2.0% vs 0.8%; p=0.031), and it might account for the increased frequency of H5 in AD patients (4.2% vs 2.3%). The complete re-sequencing of the 56 mtDNAs belonging to H5 revealed that AD patients showed a trend towards a higher number (p=0.052) of sporadic mutations in tRNA and rRNA genes when compared with controls.<h4>Conclusions</h4>Our results indicate that high resolution analysis of inherited mtDNA sequence variation can help in identifying both ancient polymorphisms defining sub-haplogroups and the accumulation of sporadic mutations associated with complex traits such as AD. |
| format |
article |
| author |
Aurelia Santoro Valentina Balbi Elisa Balducci Chiara Pirazzini Francesca Rosini Francesca Tavano Alessandro Achilli Paola Siviero Nadia Minicuci Elena Bellavista Michele Mishto Stefano Salvioli Francesca Marchegiani Maurizio Cardelli Fabiola Olivieri Benedetta Nacmias Andrea Maria Chiamenti Luisa Benussi Roberta Ghidoni Giuseppina Rose Carlo Gabelli Giuliano Binetti Sandro Sorbi Gaetano Crepaldi Giuseppe Passarino Antonio Torroni Claudio Franceschi |
| author_facet |
Aurelia Santoro Valentina Balbi Elisa Balducci Chiara Pirazzini Francesca Rosini Francesca Tavano Alessandro Achilli Paola Siviero Nadia Minicuci Elena Bellavista Michele Mishto Stefano Salvioli Francesca Marchegiani Maurizio Cardelli Fabiola Olivieri Benedetta Nacmias Andrea Maria Chiamenti Luisa Benussi Roberta Ghidoni Giuseppina Rose Carlo Gabelli Giuliano Binetti Sandro Sorbi Gaetano Crepaldi Giuseppe Passarino Antonio Torroni Claudio Franceschi |
| author_sort |
Aurelia Santoro |
| title |
Evidence for sub-haplogroup h5 of mitochondrial DNA as a risk factor for late onset Alzheimer's disease. |
| title_short |
Evidence for sub-haplogroup h5 of mitochondrial DNA as a risk factor for late onset Alzheimer's disease. |
| title_full |
Evidence for sub-haplogroup h5 of mitochondrial DNA as a risk factor for late onset Alzheimer's disease. |
| title_fullStr |
Evidence for sub-haplogroup h5 of mitochondrial DNA as a risk factor for late onset Alzheimer's disease. |
| title_full_unstemmed |
Evidence for sub-haplogroup h5 of mitochondrial DNA as a risk factor for late onset Alzheimer's disease. |
| title_sort |
evidence for sub-haplogroup h5 of mitochondrial dna as a risk factor for late onset alzheimer's disease. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2010 |
| url |
https://doaj.org/article/9894e1651792487a9699a941862fe2c6 |
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